Construction of Effective Receptor for Recognition of Avian Influenza H5N1 Protein HA1 by Assembly of Monohead Glycolipids on Polydiacetylene Vesicle Surface

Abstract

Fast and sensitive detection of epidemic virus is of the utmost importance for human being in nowadays. Various biosensors have been designed for this goal based on conjugation event between host cell glycolipids and invading virus. However, multihead glycolipids analogous to native receptors on cell surface are known to be very difficult to mimic because of the complexity of chemical synthesis. Here, we developed a new approach where two types of monohead glycolipids, active sialic acid-beta-glucoside (G1) and inactive lactose-beta-glucoside (G2), are embedded onto the surface of a polydiacetylene (PDA) vesicle to mimic native glycolipids on the cell surface. Vesicles prepared in this manner show good selectivity with a 10 ng/mL detection limit and 5 min response time to Hemagglutinin (HA1), which is more sensitive than any HA1 biosensors ever known. Moreover, in the formation of color-changeable vesicles, a very strong synergistic effect between G1 and G2 has been found, offering a novel strategy to construct effective biosensor receptors, as well as a new way to study the surface combination effect that is potentially important to the immunology study of epidemic disease.

A reinforced composite structure composed of polydiacetylene assemblies deposited on polystyrene microspheres and its application to H5N1 virus detection

“…22 The color change of the solution could be easily observed by the naked eye, as shown in Figure 8. According to the literature, the limit of detection for the target analyte was defined as a CR% value of approximately 10-15.…”

Lipid Nanoparticle‐Mediated Detection of Proteins

“…Deng et al [45] have also used a similar strategy using polydiacetylene liposomes to detect the avian infl uenza virus H5N1. The polymerizable 10,12 -pentacosadiynoic acid was used as the major component of the liposomes.…”

N-ACETYLGALACTOSAMINE BIOSENSOR BASED ON POLYDIACETYLENE NANOVESICLES: AN INDIRECTLY COLORIMETRIC RECOGNITION OF GALACTOSE-DEFICIENT IgA1

“…Using the property, PCDA-based sensors have been developed to detect biological samples, such as bacteria and viruses. [24][25][26] Recently, innovative chemical modi¯cation of PCDA systems have exploited surface binding reactions, making PCDA vesicles for chromatic sensing of molecular recognition. 27,28 Herein, we developed a noninvasive, real-time and online analysis method using PCDA nanovesicles modi¯ed with HAA as receptor that could recognize the N-acetylgalactosamine containing Oglycans on Gal-de¯cient IgA1.…”