Construction of fast xylose-fermenting yeast based on industrial ethanol-producing diploid Saccharomyces cerevisiaeby rational design and adaptive evolution

Diao, Liuyang; Liu, Yingmiao; Qian, Fengchen; Yang, Junjie; Jiang, Yu; Yang, Sheng

doi:10.1186/1472-6750-13-110

Cited by 72 publications

(64 citation statements)

References 33 publications

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“…Furthermore, adaptation strategy also stabilizes heterologous gene expression in mutant strains (Diao et al, 2013;Lee et al, 2014). In the similar way, ethanol yield has also been augmented about twofold in recombinant S. cerevisiae after adaptation in bagasse hydrolyzate containing inhibitors including furaldehydes, phenolic compounds, and aliphatic acids (Martin et al, 2007).…”

Section: −1mentioning

confidence: 67%

Evolutionary Adaptation of Kluyveromyces marxianus NIRE-K3 for Enhanced Xylose Utilization

et al. 2017

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The evolutionary adaptation was approached on the thermotolerant yeast Kluyveromyces marxianus NIRE-K3 at 45°C on xylose as a sole source of carbon for enhancement of xylose uptake. After 60 cycles, evolved strain K. marxianus NIRE-K3.1 showed comparatively 3.75-and 3.0-fold higher specific growth and xylose uptake rates, respectively, than that of native strain. Moreover, the short lag phase was also observed on adapted strain. During batch fermentation with xylose concentration of 30 g l −1, K. marxianus NIRE-K3.1 could utilize about 96% of xylose in 72 h and produced 4.67 and 15.7 g l −1 of ethanol and xylitol, respectively, which were 9.72-and 4.63-fold higher than that of native strain. Similarly, specific sugar consumption rate, xylitol, and ethanol yields were 5.07-, 1.15-, and 2.44-fold higher as compared to the native strain, respectively. The results obtained after evolutionary adaptation of K. marxianus NIRE-K3 show the significant improvement in the xylose utilization, ethanol and xylitol yields, and productivities. By understanding the results obtained, the significance of evolutionary adaptation has been rationalized, since the adapted culture could be more stable and could enhance the productivity.

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Section: −1mentioning

confidence: 67%

Evolutionary Adaptation of Kluyveromyces marxianus NIRE-K3 for Enhanced Xylose Utilization

et al. 2017

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“…Numerous follow-up studies identified extra genetic perturbations that enhanced xylose fermentation by engineered S. cerevisiae (Diao et al, 2013;Hahn-Hagerdal et al, 2007;Hasunuma et al, 2014;Kim et al, 2013b;Kim et al, 2013c;Kuyper et al, 2005a;Lee et al, 2014b;Matsushika et al, 2009;Ni et al, 2007). The step most speculated to be limiting for efficient xylose metabolism was the PPP, where xylulose-5-phosphate is metabolized into intermediates of glycolysis, fructose-6-phosphate and glyceraldehyde-3-phosphate (Diao et al, 2013;Kim et al, 2013b;Matsushika et al, 2009).…”

Section: Introductionmentioning

confidence: 94%

PHO13 deletion-induced transcriptional activation prevents sedoheptulose accumulation during xylose metabolism in engineered Saccharomyces cerevisiae

Kim

Sorek

et al. 2016

Metabolic Engineering

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“…The imbalance in redox metabolism caused by the different coenzyme preferences between XR and XDH result in the accumulation of the byproduct xylitol and a lower ethanol yield; cofactor engineering resulted in limited improvement (Hou et al 2009a; Zha et al 2012; Zhang et al 2010). For the XI pathway, since xylose is directly isomerized to xylulose with no coenzyme participation, incorporating this pathway is a direct and effective strategy for initiating xylose metabolism in S. cerevisiae (Demeke et al 2013; Diao et al 2013; Zhou et al 2012). Highly efficient XI activity is a prerequisite for rapid and efficient xylose fermentation in S. cerevisiae (Brat et al 2009; Kuyper et al 2003; Madhavan et al 2009; Walfridsson et al 1996; Zhou et al 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Normally, xylose is absorbed non-specifically and insufficiently by hexose transporters and is competitively inhibited by glucose in S. cerevisiae (Subtil and Boles 2012). Therefore, to address this problem, both wild-type and mutated endogenous and heterologous transporters were screened (Diao et al 2013; Farwick et al 2014; Moon et al 2013; Nijland et al 2014; Runquist et al 2009; Shin et al 2015; Wang et al 2015, 2016). Moreover, with higher efficiency and/or specificity for xylose, a transporter lacking glucose inhibition may relieve the glucose repression effect in a xylose absorption node, thereby increasing xylose metabolism.…”

Section: Introductionmentioning

confidence: 99%

Engineering a wild-type diploid Saccharomyces cerevisiae strain for second-generation bioethanol production

Shen

et al. 2016

Bioresour. Bioprocess.

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BackgroundThe cost-effective production of second-generation bioethanol, which is made from lignocellulosic materials, has to face the following two problems: co-fermenting xylose with glucose and enhancing the strain’s tolerance to lignocellulosic inhibitors. Based on our previous study, the wild-type diploid Saccharomyces cerevisiae strain BSIF with robustness and good xylose metabolism genetic background was used as a chassis for constructing efficient xylose-fermenting industrial strains. The performance of the resulting strains in the fermentation of media with sugars and hydrolysates was investigated.ResultsThe following two novel heterologous genes were integrated into the genome of the chassis cell: the mutant MGT05196 N360F, which encodes a xylose-specific, glucose-insensitive transporter and is derived from the Meyerozyma guilliermondii transporter gene MGT05196, and Ru-xylA (where Ru represents the rumen), which encodes a xylose isomerase (XI) with higher activity in S. cerevisiae. Additionally, endogenous modifications were also performed, including the overproduction of the xylulokinase Xks1p and the non-oxidative PPP (pentose phosphate pathway), and the inactivation of the aldose reductase Gre3p and the alkaline phosphatase Pho13p. These rationally designed genetic modifications, combined with alternating adaptive evolutions in xylose and SECS liquor (the leach liquor of steam-exploding corn stover), resulted in a final strain, LF1, with excellent xylose fermentation and enhanced inhibitor resistance. The specific xylose consumption rate of LF1 reached as high as 1.089 g g−1 h−1 with xylose as the sole carbon source. Moreover, its highly synchronized utilization of xylose and glucose was particularly significant; 77.6% of xylose was consumed along with glucose within 12 h, and the ethanol yield was 0.475 g g−1, which is more than 93% of the theoretical yield. Additionally, LF1 performed well in fermentations with two different lignocellulosic hydrolysates.ConclusionThe strain LF1 co-ferments glucose and xylose efficiently and synchronously. This result highlights the great potential of LF1 for the practical production of second-generation bioethanol.Electronic supplementary materialThe online version of this article (doi:10.1186/s40643-016-0126-4) contains supplementary material, which is available to authorized users.

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Construction of fast xylose-fermenting yeast based on industrial ethanol-producing diploid Saccharomyces cerevisiaeby rational design and adaptive evolution

Cited by 72 publications

References 33 publications

Evolutionary Adaptation of Kluyveromyces marxianus NIRE-K3 for Enhanced Xylose Utilization

Evolutionary Adaptation of Kluyveromyces marxianus NIRE-K3 for Enhanced Xylose Utilization

PHO13 deletion-induced transcriptional activation prevents sedoheptulose accumulation during xylose metabolism in engineered Saccharomyces cerevisiae

Engineering a wild-type diploid Saccharomyces cerevisiae strain for second-generation bioethanol production

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