Construction of high-resolution linkage map of the Ms locus, a restorer-of-fertility gene in onion (Allium cepa L.)

Park, Jae-Hyuk; Bang, Hyochoong; Cho, Dong Youn; Yoon, Min-Ho; Patil, Bhimanagouda S.; Kim, Sunggil

doi:10.1007/s10681-012-0851-5

Cited by 26 publications

(10 citation statements)

References 57 publications

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“…One SCAR marker and one RAPD marker were identified, which could distinguish between N and S cytoplasm in several Welsh onion cultivars, confirmed by Southern blotting (Gai & Meng 2010). Park et al (2013a) developed a high resolution linkage map of Ms locus which is involved in fertility restoration in onion. Tightly linked RAPD and CAPS markers were used to construct a fine map using F 2 populations.…”

Section: Marker For Cytoplasmic Male Sterility (Cms) In Onionmentioning

confidence: 85%

Molecular markers in the improvement of Allium crops

Chinnappareddy¹,

Khandagale²,

Chennareddy³

et al. 2013

Czech J. Genet. Plant Breed.

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Chinnappareddy L.R.D., Khandagale K., Chennareddy A., Ramappa V.G. (2013) The genus Allium (Family: Alliaceae) is the most important among the bulbous vegetable crops. Characterization of Alliums based on phenotypic traits is influenced by the environment and leads to biased diversity estimates. Recognizing the potential of DNA markers in plant breeding, researchers have adopted the molecular markers for marker-assisted selection (MAS), quantitative trait loci (QTL) mapping and characterization of different quality traits in Alliums. This review presents details about the use of DNA markers in Alliums for cultivar identification, diversity studies, SSR development, colour improvement, total soluble solids (TSS), cytoplasmic male sterility (CMS) and efforts of DNA sequencing. As there are no such reports to describe the above work under a single heading, we decided to mine literature for those who are working in onion, garlic, chives and leek improvement to generate new insights in the subject.

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Section: Marker For Cytoplasmic Male Sterility (Cms) In Onionmentioning

confidence: 85%

Molecular markers in the improvement of Allium crops

Chinnappareddy¹,

Khandagale²,

Chennareddy³

et al. 2013

Czech J. Genet. Plant Breed.

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“…This proved that marker OPT1 is not in linkage disequilibrium with the Ms locus in short-day Indian onion varieties. A previous study (G€ okc ße and Havey 2002) reported that Ms alleles and the linked RFLP marker (AOB272) which corresponds to the OPT marker were at linkage equilibrium with three open-pollinated populations due to sufficient random mating over time (Park et al 2013). As OPT was not in linkage disequilibrium with the Ms locus, we tried jnurf13 marker (Kim 2014), a codominant marker, for genotyping.…”

Section: Discussionmentioning

confidence: 99%

Limitations of PCR‐based molecular markers to identify male‐sterile and maintainer plants from Indian onion (Allium cepa L.) populations

Khar

Saini

2016

Plant Breeding

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The main aim of this study was to validate PCR markers for determining cytoplasm and genotypes at the Ms locus in short-day onion. Three cytoplasmic (OSN, MKFR and accD) and four nuclear (OPT, jnurf13, AcSKP1 and AcPMS1) markers were employed. Sel. 121-1 had 100% S cytoplasm, whereas Sel. 121-2, 'Pusa Red' and 'Pusa Madhavi' had 88%, 33% and 17% S cytoplasm, respectively. 'Early Grano' and 'Pusa Riddhi' did not possess S cytoplasm. Observations in 33 commercial varieties revealed two with sterile (S) cytoplasm. Nuclear markers were not found in linkage disequilibrium with the Ms locus, and the constitution of Ms alleles by OPT was different from other three markers, which were in conformity with each other. The other three markers predicted that most of the plants should be homozygous recessive. Anther colour also did not confirm the sterility status. It can be concluded that accD may be used for cytoplasm determination based on the ease of its use. For the Ms locus tagging, more markers are needed to be evaluated to isolate maintainer lines from open-pollinated populations.

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“…Furthermore, by randomly mating 15 F 2 heterozygous male-fertile plants, 1346 F 2:3 and 2927 F 2:4 plants were obtained, and highresolution maps were constructed. There were no recombinants between the jnurf05 marker and the Ms locus in a recombinants analysis of 4273 segregates using the jnurf05 and jnurf17 markers, indicating a very strong connection (Park et al, 2013). Using Ms locus-related molecular markers of onion onto mitotic metaphase, pachytene and super-stretched-pachytene chromosomes, tyramide FISH of genomic amplicons was physically located on chromosome 2, and the cDNA clone (666 bp) and short-genomic amplicons (846-2251 bp) were seen.…”

Section: Mapping Of G Ene S Contributing To the Re S Torer-of-male Fertilit Ymentioning

confidence: 95%

“…status (Bang et al, 2013). Polymorphic RAPD markers were converted to CAPS markers (jnurf05, jnurf06, jnurf10, jnurf17), having tight linkage with Ms locus, and these markers are of co-dominant nature, thus effectively differentiate the dominant from recessive alleles, and homozygous from heterozygous plants, and upon genome walking of an RAPD marker flanking sequence, leading to the development of dominant jnurf20 simple PCR markers (Park et al, 2013).…”

Section: Development Of Linked Molecular Markers To the Restorer Of Fertilitymentioning

confidence: 99%

Perspective and application of molecular markers linked to the cytoplasm types and male‐fertility restorer locus in onion (Allium cepa)

Manjunathagowda

2021

Plant Breeding

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Onion (Allium cepa L.; 2n = 16) belongs to the family alliaceae, and order asparagales; and the Allium is the monocot's largest genus, comprised of 920 species (Govaerts et al., 2005(Govaerts et al., -2014. Among them, onion (A. cepa L.), garlic (A. sativum L.), bunching onion (A. fistulasum L.), leek (A. porrum L.) and chives (A. schenoprosum L.) are widely being in cultivation. Among alliums, the onion has the largest economic potential, with 70% share value, followed by garlic (25%) (FAOSTAT, 2016), the consumption of onions has been steadily increasing over the world, most likely due to the nutritional benefits (Manjunathagowda et al., 2019); presence of an unique dietary flavonoids source and quercetin compounds (Slimestad et al., 2007); and medicinal therapeutic values such as lacrimatic, anti-cancer, antibiotic and anti-cholesterol activity (Grifths et al., 2002; Perez-Gregorio et al., 2010).In the world, onion acreage of 4.96 million hectare (mha) with production of 93.17 million tonnes (mt), the world's biggest onion growers are China (23.91 mt) and India (19.42 mt) (FAOSTAT, 2016).But the productivity of major onion-producing countries, China (22 tonnes per hectare (t/ha)) and India (16.18 t/ha), is low compared to Netherlands (49.70 t/ha) (National Horticultural Board, 2017), the United States (56.40 t/ha), Iran (37.95 t/ha) and Egypt (36.71 t/ha) (National Horticultural Board, 2018). This could be because of the largest onion-growing nations (China and India) have been largely covered by open-pollinated varieties, which produce poor yields per unit area and are influenced by seasonal short-day environmental conditions, as compared to countries that have adopted F 1 hybrids using the male sterility trait

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Construction of high-resolution linkage map of the Ms locus, a restorer-of-fertility gene in onion (Allium cepa L.)

Cited by 26 publications

References 57 publications

Molecular markers in the improvement of Allium crops

Molecular markers in the improvement of Allium crops

Limitations of PCR‐based molecular markers to identify male‐sterile and maintainer plants from Indian onion (Allium cepa L.) populations

Perspective and application of molecular markers linked to the cytoplasm types and male‐fertility restorer locus in onion (Allium cepa)

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