Construction of Influenza A/H1N1 Virosomal Nanobioparticles

Noori, Mahdieh; Ghorbani, Sh.; Jamali, Abbas; Shenagari, Mohammad; Hashemi, Hossein; Saghiri, Reza; Kazemi, Nooshin; Tavassoti-Kheiri, M

doi:10.21859/isv.5.2.13

Cited by 3 publications

(2 citation statements)

References 11 publications

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“…The purified viruses were harvested from the boundary of the two sucrose layers (10%/60% w/v), and dialyzed against Hank's buffer solution (HBS) overnight at 4°C to eliminate the residual sucrose. Subsequently, the dialyzed influenza viruses were sedimented by ultracentrifugation (100 000 g for 1 h at 4°C), and 5 mg of each purified virus pellet was resuspended in 375 lL of HBS (Noori et al, 2012).…”

Section: Virus Propagation and Purificationmentioning

confidence: 99%

An H1-H3 chimeric influenza virosome confers complete protection against lethal challenge with PR8 (H1N1) and X47 (H3N2) viruses in mice

et al. 2014

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Annual health threats and economic damages caused by influenza virus are still a main concern of the World Health Organization and other health departments all over the world. An influenza virosome is a highly efficient immunomodulating carrier mimicking the natural antigen presentation pathway and has shown an excellent tolerability profile due to its biocompatibility and purity. The major purpose of this study was to construct a new chimeric virosome influenza vaccine containing hemagglutinin (HA) and neuraminidase (NA) proteins derived from the A/PR/8/1934 (H1N1) (PR8) and A/X/47 (H3N2) (X47) viruses, and to evaluate its efficacy as a vaccine candidate in mice. A single intramuscular vaccination with the chimeric virosomes provided complete protection against lethal challenge with the PR8 and X47 viruses. The chimeric virosomes induced high IgG antibody responses as well as hemagglutination inhibition (HAI) titers. HAI titers following the chimeric virosome vaccination were at the same level as the whole inactivated influenza vaccine. Mice immunized with the chimeric virosomes displayed considerably less weight loss and exhibited significantly reduced viral load in their lungs compared with the controls. The chimeric virosomes can be used as an innovative vaccine formulation to confer protection against a broad range of influenza viruses.

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Section: Virus Propagation and Purificationmentioning

confidence: 99%

An H1-H3 chimeric influenza virosome confers complete protection against lethal challenge with PR8 (H1N1) and X47 (H3N2) viruses in mice

et al. 2014

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“…The purification was carried out by stepwise sucrose equilibrium density gradient ultracentrifugation ( 10 -60% ; w/v) for (100'000 x g, 4°C, 1.5 h). The final purified virus particles were harvested from the boundary of the two sucrose layers and dialyzed against HBS at 4°C overnight using a Slide-A-Lyzer dialysis cassette with 10-kDa protein MW cut-off to remove the residual trace of sucrose [24].…”

Section: Concentration and Purification Tangential Flow Filtrationmentioning

confidence: 99%

Optimization of Microcarrier-based MDCK-SIAT1 Culture System for Influenza Virus Propagation

Abdoli

Soleimanjahi

Jamali

et al. 2014

vacres

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Introduction:The preparation of seasonal influenza virus vaccines and especially its large-scale production requirement after the emergence or reemergence of a pandemic will need an alternative host cell system due to current suboptimal methods and the insufficiency of embryonated chicken eggs needed for producing them. In response to the vital and increasing demand for alternative means for influenza vaccine production, a cell line culture on microcarriers could be a potential alternative to the egg-based production. Methods: Influenza A/PR/8/1934 H1N1 was purified and quantified by plaque assay. The purified virus with 0.01 multiplicity of infection (MOI) was inoculated on Madin-Darby canine kidney-Siat1 (MDCK-SIAT1) cell line. Cytodex-1 microcarrier beads (2 g/l and 2.0×10 5 cells/ml) were used in a spinner flask to culture MDCK-SIAT1cells .The culture medium was harvested and clarified and the virus yield was quantified by 50% cell culture infective dose ( CCID 50 ) and hemagglutination assays. Next, the virus was concentrated and purified by ultra-filtration and ultra-centrifugation, respectively. Results: MDCK-SIAT1cells attached to the microcarriers and the cell numbers were increased efficiently. The cellular yield from the microcarrier culture was 2×10 6 cells/ml after 4-5 days. The yield of the virus titer measured by CCID 50 and hemagglutination assays after the clarification was 108 CCID 50 / ml and 40960 HA unit/ml, respectively. Conclusion: MDCK-SIAT1cells may be considered as a new substrate for the production of influenza vaccines. Using Cytodex-1 microcarrier beads can be an appropriate strategy to improve the viral yield and to lower the cost of influenza vaccine production.

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Preparation and Characterization of Influenza Virosomes Using Nonionic, Dialyazble Phospholipid for Efficient Membrane Solubilization and Reconstitution

Kumar

Jain

et al. 2020

Springer Proceedings in Physics

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Construction of Influenza A/H1N1 Virosomal Nanobioparticles

Abstract: Background and Aims:

Cited by 3 publications

References 11 publications

An H1-H3 chimeric influenza virosome confers complete protection against lethal challenge with PR8 (H1N1) and X47 (H3N2) viruses in mice

An H1-H3 chimeric influenza virosome confers complete protection against lethal challenge with PR8 (H1N1) and X47 (H3N2) viruses in mice

Optimization of Microcarrier-based MDCK-SIAT1 Culture System for Influenza Virus Propagation

Preparation and Characterization of Influenza Virosomes Using Nonionic, Dialyazble Phospholipid for Efficient Membrane Solubilization and Reconstitution

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