Construction of lipid layer and monitoring its digestion by optical interferometry

Real-time monitoring of the hydrolysis of vegetable oils loaded in silica colloidal crystal films with lipase by optical interferometry

“…Using ordered porous layer interferometry (OPLI), previous works have loaded TAGs into alkylated SCC films, enabling the measurement of changes in the lipid layer optical thickness (OT), which provided a new method for studying the hydrolysis of water-insoluble lipids in heterogeneous phases. 33 In this work, the enzymatic hydrolysis processes of four kinds of vegetable oils were monitored in real-time and the health value of different vegetable oils was revealed from the aspect of their hydrolysis behavior (Scheme 1). This system can monitor the vegetable oil's enzymatic hydrolysis process with the advantages of being label-free, convenient, low-cost, real-time, and sensitive.…”

Real-Time Monitoring of Curcumin Release with a Lipid–Curcumin-Loaded Silica Colloidal Crystal Film Using Optical Interferometry

“…A label-free biosensing system based on OPLI has been developed in recent years. 20,21 The interference fringes of the SCC film depend on the product of the physical thickness d and the average refractive index n (optical thickness, OT). 22,23 An interaction involving lipase triggered a decrease of mass in the lipid loaded within the SCC film, resulting in changes in the refractive index of the layered medium, which is detected as a corresponding shift in the interference pattern.…”

“…28 As an effective "scaffold", the SCC film with the hydrophobic treatment provided effective structural support for lipid payload. 20 An OPLI system was developed for in situ and real-time monitoring of lipid digestion with a lipid-loaded SCC layer in the previous work. 20 Lipids were degraded and released to form small droplets dispersed in an aqueous solution based on lipolysis.…”

“…Reflection of white light at the top and bottom of the SCC layer results in an interference pattern. A label-free biosensing system based on OPLI has been developed in recent years. , The interference fringes of the SCC film depend on the product of the physical thickness d and the average refractive index n (optical thickness, OT). , An interaction involving lipase triggered a decrease of mass in the lipid loaded within the SCC film, resulting in changes in the refractive index of the layered medium, which is detected as a corresponding shift in the interference pattern. Compared with porous silicon, anodized aluminum, and thin-film substrates used for optical interferometry, − the SCC film had the advantages of the adjustable SCC gap and controllable SCC film thickness .…”

“…The SCC film combined a flow injection system with a spectral acquisition system, which was suitable for focusing molecular interaction in complex environments . As an effective “scaffold”, the SCC film with the hydrophobic treatment provided effective structural support for lipid payload . An OPLI system was developed for in situ and real-time monitoring of lipid digestion with a lipid-loaded SCC layer in the previous work .…”