Construction of Plasmid Vector Systems for Clostridium Acetobutylicum

Brehm, John K.; Oultram, John D.; Thompson, D.E.; Swinfield, T.J.; Peck, Hazel; Minton, Nigel P.; Young, Malcolm P.

doi:10.1016/b978-0-12-274161-6.50073-5

Cited by 4 publications

(1 citation statement)

References 5 publications

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“…The C. beijerinckii spo0A gene was isolated as two contiguous Eco RI– Pst I fragments of genomic DNA. A 1244 bp Eco RI– Pst I fragment, which includes the C‐terminus of spoIVB , the intergenic region between spoIVB and spo0A and the N‐terminus of spo0A , was cloned in pMTL20EC (Brehm et al ., 1988) to generate p0A1. A 1.5 kbp Eco RI– Pst I fragment including the C‐terminus of spo0A was cloned in pMTL20 (Chambers et al ., 1988) to generate p0A2.…”

Section: Methodsmentioning

confidence: 99%

Spo0A directly controls the switch from acid to solvent production in solvent‐forming clostridia

Ravagnani

Jennert

Steiner

et al. 2000

Molecular Microbiology

137

128

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SummaryThe spo0A genes of Clostridium beijerinckii NCIMB 8052 and Clostridium cellulolyticum ATCC 35319 were isolated and characterized. The C-terminal DNA-binding domains of the predicted products of spo0A from these two organisms, as well as 16 other taxonomically diverse species of Bacillus and Clostridium, show extensive amino acid sequence conservation (56% identity, 65% similarity over 104 residues). A 12-amino-acid motif (SRVERAIRHAIE) that forms the putative DNA recognition helix is particularly highly conserved, suggesting a common DNA target.

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Section: Methodsmentioning

confidence: 99%

Spo0A directly controls the switch from acid to solvent production in solvent‐forming clostridia

Ravagnani

Jennert

Steiner

et al. 2000

Molecular Microbiology

137

128

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In Bacillus subtilis 168, teichoic acid of the cross-wall may be different from that of the cylinder: a hypothesis based on transcription analysis of tag genes

Mauël¹,

Bauduret²,

Chervet³

et al. 1995

Microbiology

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Five of the genes known to encode the enzymes for the synthesis of poly(glycerol phosphate), the major teichoic acid of Bacillus subti/is 168, are organized in two divergently transcribed operons, tagAB and tagD€F. lacZ and gus transcriptional fusions to the first genes of these operons revealed that: (i) in media of different richness, higher growth rates were paralleled by lower transcription levels; (ii) upon transition to stationary phase, the transcription per unit mass of both operons increased abruptly by a factor of about two;and (iii) a rise in temperature was accompanied by decreased transcription of tagA and increased transcription of tagD. Mapping of transcription start points revealed two divergent aA-controlled promoters. Although tagD and the neighbouring downstream gene tag€ are transcribed from the same promoter, the latter was expressed at a much lower level than the former. Moreover, expression of tag€, and of the translationally coupled tagF, did not increase at the onset of the stationary phase, indicating that additional regulatory signals may act in the intergenic tagD-tag€ region. Optimal transcription of these operons appears to require the entire regulatory region, suggesting that tag gene expression may, among other factors, be regulated by the threedimensional configuration of this segment. The biological implications of these results are discussed.

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Molecular genetics and the initiation of solventogenesis inClostridium beijerinckii(formerlyClostridium acetobutylicum) NCIMB 8052

et al. 1995

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A physical map of the Clostridium beijerinckii (formerly Clostridium acetobutylicum) NCIMB 8052 chromosome has been constructed, encompassing about 90 rare restriction sites. The 14 rrn operons together with about 40 genes have been assigned positions on the map. Genetic analysis and gene transfer have been developed in this organism to enable in vivo analysis of the roles of cloned genes using marker replacement technology. Experiments using the available genetic tools have shown that spo0A plays a cardinal role in controlling several aspects of the transition from exponential growth to stationary phase in C. beijerinckii. These include initiation of sporulation, accumulation of the storage polysaccharide, granulose, and production of acetone and butanol. Several C. beijerinckii and C. acetobutylicum genes concerned with fermentative metabolism, whose expression is modulated at the onset of solventogenesis, contain sequence motifs resembling 0A boxes in their 5' regulatory regions. This invites the speculation that they are under the direct control of Spo0A, and additional data are now required to test this prediction.

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Construction of Plasmid Vector Systems for Clostridium Acetobutylicum

Cited by 4 publications

References 5 publications

Spo0A directly controls the switch from acid to solvent production in solvent‐forming clostridia

Spo0A directly controls the switch from acid to solvent production in solvent‐forming clostridia

In Bacillus subtilis 168, teichoic acid of the cross-wall may be different from that of the cylinder: a hypothesis based on transcription analysis of tag genes

Molecular genetics and the initiation of solventogenesis inClostridium beijerinckii(formerlyClostridium acetobutylicum) NCIMB 8052

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