Construction of scaffolds composed of acellular cardiac extracellular matrix for myocardial tissue engineering

Pourfarhangi, Kamyar Esmaeili; Mashayekhan, Shohreh; Asl, Sasan Ghanbari; Hajebrahimi, Zahra

doi:10.1016/j.biologicals.2018.03.005

Cited by 36 publications

(27 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pok et al modified the patch by adding a polycaprolactone (PCL) core, and tested the therapeutic potential of the patch in a rat infarction model . Pourfarhangi et al evaluated the optimized ratio of cECM and chitosan for improving CPC function, and determined that higher cECM composition in composite patches improved CPC viability . Efraim et al evaluated pECM with genipin crosslinking and chitosan addition, with the intention of therapeutic injection rather than patch implantation .…”

Section: Soluble Decmmentioning

confidence: 99%

Decellularized Extracellular Matrix Materials for Cardiac Repair and Regeneration

Bejleri

Davis

2019

Adv Healthcare Materials

158

105

View full text Add to dashboard Cite

Decellularized extracellular matrix (dECM) is a promising biomaterial for repairing cardiovascular tissue, as dECM most effectively captures the complex array of proteins, glycosaminoglycans, proteoglycans, and many other matrix components that are found in native tissue, providing ideal cues for regeneration and repair of damaged myocardium. dECM can be used in a variety of forms, such as solid scaffolds that maintain native matrix structure, or as soluble materials that can form injectable hydrogels for tissue repair. dECM has found recent success in many regeneration and repair therapies, such as for musculoskeletal, neural, and liver tissues. This review focuses on dECM in the context of cardiovascular applications, with variations in tissue and species sourcing, and specifically discusses advances in solid and soluble dECM development, in vitro studies, in vivo implementation, and clinical translation.

show abstract

Section: Soluble Decmmentioning

confidence: 99%

Decellularized Extracellular Matrix Materials for Cardiac Repair and Regeneration

Bejleri

Davis

2019

Adv Healthcare Materials

158

105

View full text Add to dashboard Cite

show abstract

“…In the previous studies (Nasiri and Mashayekhan, 2017;Sivandzade and Mashayekhan, 2018;Esmaeili Pourfarhangi et al, 2018), which the same protocol was used to extract ECM from the native tissue, the success of decellularization process was confirmed using Hematoxylin & Eosin staining test. In this study DNA content of ECM was determined.…”

Section: Dna Contentmentioning

confidence: 91%

“…Hydrogel samples were fabricated according to a certain protocol, which was optimized for cardiac tissue engineering applications and reported by Esmaeili et al (Esmaeili Pourfarhangi et al, 2018). Briefly, a chitosan solution (3.5% wt.)…”

Section: Hydrogel Fabricationmentioning

confidence: 99%

Reinforcement of a decellularized extracellular matrix-derived hydrogel using nanofibers for cardiac tissue engineering

Mashayekhan

Jahanshahi

Moghadam

2020

Int. J. Adv. Biol. Biomed. Res.

View full text Add to dashboard Cite

The role of heart disease in increasing worldwide death and the limited availability of organs for transplantation have encouraged multiple strategies to fabricate functional and implantable constructs. One of these strategies is to develop a biologically similar heart tissue scaffold, in which two types of fiber and hydrogel are commonly used. Toward this goal, taking advantage of both hydrogels properties and fibers features with excellent mechanical properties can be considered as a promising method. The purpose of this study was to develop a fiber/hydrogel composite of gelatin, poly-caprolactone (PCL), cardiac extracellular matrix (ECM), and chitosan. The fibrous scaffolds of PCL and gelatin were characterized by SEM, water drop contact angle test, FTIR, and mechanical tests. The results showed that the average diameter of nanofibers, hydrophilicity and mechanical properties of the fibrous scaffolds increased with increasing the gelatin content in the spinning solution. Furthermore, the results of mechanical tests indicated that by integrating fibers with gelatin to PCL mass ratio of 2 in the hydrogel of chitosan and ECM with a mass ratio equal to 1, we obtained a construct with similar mechanical properties to native heart tissue, which may be proposed as an appropriate scaffold for heart tissue engineering.

show abstract

“…Thereby, this scaffold was able to stimulate cell proliferation, to increase actin filaments and to improve integrin β1 expression, representing a novel platform to better study and understand, cell-cell or cell-matrix interactions and, possibly, to improve cardiac valve tissue engineering [109] . As said previously, native ECM could represent a suitable mechanical and biological environment able to ensure cell engraftment and to improve therapeutic and regenerative properties of resident and transplanted cells [29,110,111] . Several studies demonstrated the positive effects of cardiac dECM patch in damaged cardiac tissue.…”

Section: D Scaffoldsmentioning

confidence: 94%

CPCs and ECM: A Good Mix for Cardiac Regeneration

2018

Med One

View full text Add to dashboard Cite

Despite several improvements in term of diagnosis and prevention, ischemic heart disease still represents one of the principal worldwide causes of death. Cardiac progenitor cells (CPCs) based therapy is considered a valid alternative to heart transplant, but several issues concerning the transplanted cells viability, retention and therapeutic effect need to be solved. Tissue engineering, mixing synthetic or natural polymers with injected cells, could represent the way through which overcomes shortages and set up an effective cardiac regenerative therapy. Nowadays, it is well known that cardiac extracellular matrix (ECM) provides structural and functional integrity, affects cardiac function, development and physiologic repair. In this optic, ECM and ECM-like materials represent functional and biocompatible tools with a great potential to serve as natural or nature-mimicking scaffolds in the field of regenerative medicine. The aim of the present work is to provide an overview on the state of the art and recent advantages on CPCs and scaffold-based therapy for heart regeneration.

show abstract

Construction of scaffolds composed of acellular cardiac extracellular matrix for myocardial tissue engineering

Cited by 36 publications

References 46 publications

Decellularized Extracellular Matrix Materials for Cardiac Repair and Regeneration

Decellularized Extracellular Matrix Materials for Cardiac Repair and Regeneration

Reinforcement of a decellularized extracellular matrix-derived hydrogel using nanofibers for cardiac tissue engineering

CPCs and ECM: A Good Mix for Cardiac Regeneration

Contact Info

Product

Resources

About