Construction of vascularized tissue-engineered breast with dual angiogenic and adipogenic micro-tissues

Ni, Ruopiao; Luo, Chao; Ci, Hai; Sun, Di; An, Ran; Wang, Zhenxing; Yang, Jie; Li, Yiqing; Sun, Jiaming

doi:10.1016/j.mtbio.2022.100539

Cited by 11 publications

(5 citation statements)

References 48 publications

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“…And immunohistochemical staining showed that the seeded chondrocytes were distributed in the center and the seeded BMSCs were distributed in the periphery area of the explant (Figure 5H–J ). The oil‐water system is typically used in fabricating bioactive scaffolds for tissue engineering, [ 27 ] The residual organic solvents can be removed from the scaffold by washing with PBS or sterilized water before cell seeding. Compared to traditional vertebral fusion or disc replacement, the spinal column tissue made by liquid blocks provides a new segmented spinal column replacement with good integration of vertebra and intervertebral disc.…”

Section: Resultsmentioning

confidence: 99%

Reconfigurable Magnetic Liquid Building Blocks for Constructing Artificial Spinal Column Tissues

et al. 2023

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All‐liquid molding can be used to transform a liquid into free‐form solid constructs, while maintaining internal fluidity. Traditional biological scaffolds, such as cured pre‐gels, are normally processed in solid state, sacrificing flowability and permeability. However, it is essential to maintain the fluidity of the scaffold to truly mimic the complexity and heterogeneity of natural human tissues. Here, this work molds an aqueous biomaterial ink into liquid building blocks with rigid shapes while preserving internal fluidity. The molded ink blocks for bone‐like vertebrae and cartilaginous‐intervertebral‐disc shapes, are magnetically manipulated to assemble into hierarchical structures as a scaffold for subsequent spinal column tissue growth. It is also possible to join separate ink blocks by interfacial coalescence, different from bridging solid blocks by interfacial fixation. Generally, aqueous biomaterial inks are molded into shapes with high fidelity by the interfacial jamming of alginate surfactants. The molded liquid blocks can be reconfigured using induced magnetic dipoles, that dictated the magnetic assembly behavior of liquid blocks. The implanted spinal column tissue exhibits a biocompatibility based on in vitro seeding and in vivo cultivating results, showing potential physiological function such as bending of the spinal column.

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Section: Resultsmentioning

confidence: 99%

Reconfigurable Magnetic Liquid Building Blocks for Constructing Artificial Spinal Column Tissues

et al. 2023

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“…Overall, our findings present a novel approach for the engineering of ASC, highlighting the potential of utilizing monomeric CXCL12 to enhance paracrine mechanisms for the treatment of stroke (Figure 7). ASCs play a pivotal role in stimulating angiogenesis through the secretion of VEGF, suggesting their potential in wound healing [19][20][21]. Previous studies have shown that ASC transplantation could alleviate neurological functional deficits, decrease infarct volume and brain atrophy, and reduce apoptosis and inflammation in cerebral vascular disease [22][23][24].…”

Section: Discussionmentioning

confidence: 99%

Monomeric CXCL12-Engineered Adipose-Derived Stem Cells Transplantation for the Treatment of Ischemic Stroke

Zheng,

Haroon,

Liu

et al. 2024

IJMS

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Adipose-derived stem cells (ASCs) possess therapeutic potential for ischemic brain injury, and the chemokine CXCL12 has been shown to enhance their functional properties. However, the cumulative effects of ASCs when combined with various structures of CXCL12 on ischemic stroke and its underlying molecular mechanisms remain unclear. In this study, we genetically engineered mouse adipose-derived ASCs with CXCL12 variants and transplanted them to the infarct region in a mice transient middle cerebral artery occlusion (tMCAO) model of stroke. We subsequently compared the post-ischemic stroke efficacy of ASC-mCXCL12 with ASC-dCXCL12, ASC-wtCXCL12, and unmodified ASCs. Neurobehavior recovery was assessed using modified neurological severity scores, the hanging wire test, and the elevated body swing test. Changes at the tissue level were evaluated through cresyl violet and immunofluorescent staining, while molecular level alterations were examined via Western blot and real-time PCR. The results of the modified neurological severity score and cresyl violet staining indicated that both ASC-mCXCL12 and ASC-dCXCL12 treatment enhanced neurobehavioral recovery and mitigated brain atrophy at the third and fifth weeks post-tMCAO. Additionally, we observed that ASC-mCXCL12 and ASC-dCXCL12 promoted angiogenesis and neurogenesis, accompanied by an increased expression of bFGF and VEGF in the peri-infarct area of the brain. Notably, in the third week after tMCAO, the ASC-mCXCL12 exhibited superior outcomes compared to ASC-dCXCL12. However, when treated with the CXCR4 antagonist AMD3100, the beneficial effects of ASC-mCXCL12 were reversed. The AMD3100-treated group demonstrated worsened neurological function, aggravated edema volume, and brain atrophy. This outcome is likely attributed to the interaction of monomeric CXCL12 with CXCR4, which regulates the recruitment of bFGF and VEGF. This study introduces an innovative approach to enhance the therapeutic potential of ASCs in treating ischemic stroke by genetically engineering them with the monomeric structure of CXCL12.

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“…With the development of personalized biological breast implants, 8 these innovative surgical approaches may bring us close to a very high‐quality postoperative result, with the hope that the only constraint on preventive surgery in the near future will be purely functional and linked to the birth plan (pregnancy and breastfeeding), rather than aesthetic.…”

Section: Figurementioning

confidence: 99%

Scarless preventive surgery

Rathat,

Blay,

Bakenga

et al. 2023

Intl J Gynecology & Obste

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Construction of vascularized tissue-engineered breast with dual angiogenic and adipogenic micro-tissues

Cited by 11 publications

References 48 publications

Reconfigurable Magnetic Liquid Building Blocks for Constructing Artificial Spinal Column Tissues

Reconfigurable Magnetic Liquid Building Blocks for Constructing Artificial Spinal Column Tissues

Monomeric CXCL12-Engineered Adipose-Derived Stem Cells Transplantation for the Treatment of Ischemic Stroke

Scarless preventive surgery

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