Under the development of interdisciplinary fusion, user research has been greatly influenced by technology-driven neuroscience and sensory science, in terms of thinking and methodology. The use of technical methods, such as EEG and eye-tracking, has gradually become a research trend and hotspot in this field, in order to explore the deep cognitive states behind users’ objective behaviors. This review outlines the applications of EEG and eye-tracking technology in the field of user research, with the aim of promoting future research and proposing reliable reference indicators and a research scope. It provides important reference information for other researchers in the field. The article summarizes the key reference indicators and research paradigms of EEG and eye-tracking in current user research, focusing on the user research situation in industrial products, digital interfaces and spatial environments. The limitations and research trends in current technological applications are also discussed. The feasibility of experimental equipment in outdoor environments, the long preparation time of EEG experimental equipment, and the accuracy error of physiological signal acquisition are currently existing problems. In the future, research on multi-sensory and behavioral interactions and universal studies of multiple technology fusions will be the next stage of research topics. The measurement of different user differentiation needs can be explored by integrating various physiological measurements such as EEG signals and eye-tracking signals, skin electrical signals, respiration, and heart rate.