Consumption of Bitter Alkaloids in Drosophila melanogaster in Multiple-Choice Test Conditions

Sellier, Marie-Jeanne; Reeb, Pablo; Marion‐Poll, Frédéric

doi:10.1093/chemse/bjq133

Cited by 60 publications

(74 citation statements)

References 52 publications

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“…For example, bitter chemicals inhibit sugar detection in insects of different groups (Morita, 1959;Dethier, 1980Dethier, , 1987Schoonhoven, 1982;Dethier andBowdan, 1989, 1992;Chapman et al, 1991;Schoonhoven and Liner, 1994), including Drosophila (Meunier et al, 2003a; Lee et al, , 2012Sellier and Marion-Poll, 2011;Jeong et al, 2013) in which other interactions were described recently, such as between sugars and acids (Charlu et al, 2013;Chen and Amrein, 2014). Whereas the molecular basis of bitter suppression of sugar detection is still under scrutiny and may involve odorant binding proteins (OBPs;Jeong et al, 2013;Swarup et al, 2014), the behavioral role of such interactions have been considered rarely (König et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Dual Mechanism for Bitter Avoidance inDrosophila

et al. 2015

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In flies and humans, bitter chemicals are known to inhibit sugar detection, but the adaptive role of this inhibition is often overlooked. At best, this inhibition is described as contributing to the rejection of potentially toxic food, but no studies have addressed the relative importance of the direct pathway that involves activating bitter-sensitive cells versus the indirect pathway represented by the inhibition of sugar detection. Using toxins to selectively ablate or inactivate populations of bitter-sensitive cells, we assessed the behavioral responses of flies to sucrose mixed with strychnine (which activates bitter-sensitive cells and inhibits sugar detection) or with L-canavanine (which only activates bitter-sensitive cells). As expected, flies with ablated bitter-sensitive cells failed to detect L-canavanine mixed with sucrose in three different feeding assays (proboscis extension responses, capillary feeding, and two-choice assays). However, such flies were still able to avoid strychnine mixed with sucrose. By means of electrophysiological recordings, we established that bitter molecules differ in their potency to inhibit sucrose detection and that sugar-sensing inhibition affects taste cells on the proboscis and the legs. The optogenetic response of sugar-sensitive cells was not reduced by strychnine, thus suggesting that this inhibition is linked directly to sugar transduction. We postulate that sugar-sensing inhibition represents a mechanism in insects to prevent ingesting harmful substances occurring within mixtures.

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Section: Introductionmentioning

confidence: 99%

Dual Mechanism for Bitter Avoidance inDrosophila

et al. 2015

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“…This is especially critical given recent heightened interest in the biological mechanisms underlying feeding control in Drosophila and the number of different implementations of various feeding assays, most of which assess preference only at a single time point (Melcher and Pankratz, 2005;Gordesky-Gold et al, 2008;Moon et al, 2009;Ribeiro and Dickson, 2010;Vargas et al, 2010;Vigne and Frelin, 2010;Dus et al, 2011;Sellier et al, 2011;Weiss et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Capillary feeder (CAFE) assays were performed similar to those in previous reports (Ja et al, 2007;Devineni and Heberlein, 2009;Sellier et al, 2011). For all assays, eight female flies were placed in a 15 ml conical vial with four holes in the lid fitted with cut 200 l pipette tips to hold capillaries.…”

Section: Methodsmentioning

confidence: 99%

“…To measure feeding preferences over an extended time period, we presented flies with a choice of 60% sucrose and 40% mannose versus 100% L-fucose using a modified version of the CAFE assay (Ja et al, 2007;Devineni and Heberlein, 2009;Sellier et al, 2011). Importantly, both choices were presented at the same total concentration (50 mM) to control for any effects of osmolality.…”

Section: Flies Shift Feeding Preference Toward Caloric Sugarsmentioning

confidence: 99%

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Integration of Taste and Calorie Sensing inDrosophila

et al. 2012

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Animals use gustatory information to assess the suitability of potential food sources and make critical decisions on what to consume. For example, the taste of sugar generally signals a potent dietary source of carbohydrates. However, the intensity of the sensory response to a particular sugar, or "sweetness," is not always a faithful reporter of its nutritional value, and recent evidence suggests that animals can sense the caloric content of food independently of taste. Here, we demonstrate that the vinegar fly Drosophila melanogaster uses both taste and calorie sensing to determine feeding choices, and that the relative contribution of each changes over time. Using the capillary feeder assay, we allowed flies to choose between sources of sugars that varied in their ratio of sweetness to caloric value. We found that flies initially consume sugars according to taste. However, over several hours their preference shifts toward the food source with higher caloric content. This behavioral shift occurs more rapidly following food deprivation and is modulated by cAMP and insulin signaling within neurons. Our results are consistent with the existence of a taste-independent calorie sensor in flies, and suggest that calorie-based reward modifies long-term feeding preferences.

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“…NaCl was tested at 10, 100 and 500 mM concentrations, while fructose, a natural sweet present in Citrus fruits (Rodriguez-Saona et al 2001), was tested at 1, 10 and 50 mM concentrations. The following bitter stimuli were also assayed: 5,6-dihydro-9,10-dimethoxybenzo [g]-1,3-benzodioxolo[5,6-a]quinolizinium or berberine chloride hydrate, 3,3ʹ,4ʹ,5,7-pentaidrossiflavone or quercetin dehydrate, 6-n-propyl-thiouracil or PROP and nicotine at concentrations of 0.01, 0.1 and 1 mM (Liscia & Solari 2000;Sellier et al 2011;Weiss et al 2011;Masala et al 2014). The flavonoid quercetin is found in immature apple, lemon and grapefruit juice (Drewnowski & Gomez-Carneros 2000).…”

Section: Stimulimentioning

confidence: 99%

Morphological and electrophysiological analysis of tarsal sensilla in the medfly Ceratitis capitata (Wiedemann, 1824) (Diptera: Tephritidae)

Loy

Solari

Isola

et al. 2016

Italian Journal of Zoology

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The Mediterranean fruit fly, Ceratitis capitata (Wiedemann, 1824) (Diptera: Tephritidae), is a polyphagous pest in horticulture, mainly targeting pomaceous and citrus fruits. To gain better knowledge about its chemosensory system related to taste, essential for behavioural strategies and localisation of host plants, we examined the tarsal external morphology by highresolution scanning electron microscopy, focusing on sensilla. Numerous trichoid and chaetica sensilla, related to taste and mechanoreceptor systems, and other types of sensilla are present in tarsal segments. The responses of specific trichoid chemosensilla were also studied through electrophysiological experiments by stimulation with sodium chloride, fructose and four bitter stimuli. Electrophysiological data allowed us to distinguish three types of different neurons: high ("H"), middle ("M") and small ("S"). Increasing concentrations of sodium chloride enhanced the activity of H cells, while fructose boosted M cells activity. On the contrary, S cells showed no increase in spike activity after stimulation with these substances. Bitter stimuli induced no significant response on these cells. Behavioural assays showed no statistical difference among the responses to sugars, bitter substances and citric acid. Our results on taste stimuli will be useful in increasing knowledge of Ceratitis chemoreception, fundamental in new integrated pest management.

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Consumption of Bitter Alkaloids in Drosophila melanogaster in Multiple-Choice Test Conditions

Cited by 60 publications

References 52 publications

Dual Mechanism for Bitter Avoidance inDrosophila

Dual Mechanism for Bitter Avoidance inDrosophila

Integration of Taste and Calorie Sensing inDrosophila

Morphological and electrophysiological analysis of tarsal sensilla in the medfly Ceratitis capitata (Wiedemann, 1824) (Diptera: Tephritidae)

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