Consumption of elicited soybean sprout flour increases calcium level in serum of ovariectomized rats

Abstract

<p>Background<br />The decline in estrogens could lead to an imbalance of bone formation and absorption as well as bone mineralization. Phytoestrogens are known to have the potential to maintain bone health. Calcium and phosphorus are essential components for bone mineralization. This study aimed to investigate the effect of soybean sprout flour consumption on serum calcium and phosphorus levels in ovariectomized rats.</p><p>Methods<br />A randomized post-test-only control group design involving 25 female Sprague-Dawley rats aged 2 months, which were divided into five groups: (i) normal controls on standard feed diet AIN-93 (C-N), (ii) ovariectomy controls on standard feed diet (OVX -N); (iii) ovariectomized rats on standard feed diet + ethinylestradiol (OVX -E), (iv) ovariectomized rats on standard feed diet + soybean flour (OVX -S); (v) ovariectomized rats on standard feed diet + soybean sprout flour (OVX -SS). The rats were treated for 6 weeks, then on the last day of treatment blood sampling was conducted. Analysis of calcium and phosphorus in serum was performed using the Arsenazo III photometric method. Data were analyzed using one-way Anova.</p><p>Results<br />Mean serum calcium level was highest in the OVX-SS group (12.59 mg/dL ± 1.32), while mean serum phosphorus level was highest in the C-N group (1.708 mg/dL ± 0.28). Soybean sprout flour consumption had a significant effect on serum calcium levels (p=0.019), yet it had no effect on body weight and serum phosphorus levels of the rats.</p><p>Conclusion<br />Consumption of soybean sprout flour could positively affect serum calcium levels in rats.</p>

Evaluation of La(XT), a novel lanthanide compound, in an OVX rat model of osteoporosis

“…The literature suggests that a decrease in plasma calcium level and an increase in phosphorus level in OVX rats with osteoporosis, we observed no change regarding plasma calcium and phosphorus levels in the current study ( Fig. 3 ) ( Elkomy and Elsaid, 2015 ; Aminah et al, 2017 ). It was not clear that why the phosphorus level declined in all experimental animals during the study.…”

Calcium Bioavailability and Serum Calcium Level in Pregnant Rats After Administration of Milk-Based Drinks Containing Lactic Acid Bacteria

“…The ANOVA test results showed that the induction of undernutrition had a significant effect on serum calcium level. The groups A1, A2, and A3 had significantly lower level of serum calcium than the control group (A0), whereas A3 had the lowest level of serum calcium when compared to all the other groups These findings was supported by a previous study conducted by Aminah et al (2017) who concluded that normal level of serum calcium in two months Sprague Dawley rats was 11.36±0.46 mg/dl. In this research, the A1, A2, and A3 had a lower serum calcium as much as 17.08%, 19.45%, and 31.55%, respectively when compared to the control group (A0).…”