Contactless dielectrophoretic manipulation of biological cells using pulsed magnetic fields

Novickij, Vitalij; Grainys, Audrius; Novickij, Jurij

doi:10.1049/iet-nbt.2012.0039

Cited by 11 publications

(9 citation statements)

References 14 publications

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“…The 8 kV/cm electric field strength ( E ) has been achieved using the 800 V electrical pulse in a 1 mm electrode gap cuvette. The spatial distribution of the magnetic field was evaluated using the finite element method (FEM) analysis in Comsol Multiphysics software (COMSOL, Stockholm, Sweden) 63 64 . The coil was driven by single current pulse described as a time function with the peak amplitude of 10.2 kA and duration of 1.2 ms ( Fig.…”

Section: Methodsmentioning

confidence: 99%

Pulsed Electromagnetic Field Assisted in vitro Electroporation: A Pilot Study

Novickij

Grainys

Lastauskienė

et al. 2016

Sci Rep

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Electroporation is a phenomenon occurring due to exposure of cells to Pulsed Electric Fields (PEF) which leads to increase of membrane permeability. Electroporation is used in medicine, biotechnology, and food processing. Recently, as an alternative to electroporation by PEF, Pulsed ElectroMagnetic Fields (PEMF) application causing similar biological effects was suggested. Since induced electric field in PEMF however is 2–3 magnitudes lower than in PEF electroporation, the membrane permeabilization mechanism remains hypothetical. We have designed pilot experiments where Saccharomyces cerevisiae and Candida lusitaniae cells were subjected to single 100–250 μs electrical pulse of 800 V with and without concomitant delivery of magnetic pulse (3, 6 and 9 T). As expected, after the PEF pulses only the number of Propidium Iodide (PI) fluorescent cells has increased, indicative of membrane permeabilization. We further show that single sub-millisecond magnetic field pulse did not cause detectable poration of yeast. Concomitant exposure of cells to pulsed electric (PEF) and magnetic field (PMF) however resulted in the increased number PI fluorescent cells and reduced viability. Our results show increased membrane permeability by PEF when combined with magnetic field pulse, which can explain electroporation at considerably lower electric field strengths induced by PEMF compared to classical electroporation.

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Section: Methodsmentioning

confidence: 99%

Pulsed Electromagnetic Field Assisted in vitro Electroporation: A Pilot Study

Novickij

Grainys

Lastauskienė

et al. 2016

Sci Rep

Self Cite

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“…Comparison between the experimental research conducted by V. Novickij et al [47] and the simulation result from our model. (a) The damped sinusoidal current signal applied to the coil.…”

Section: Resultsmentioning

confidence: 81%

Simulation study of Cell Transmembrane Potential and Electroporation Induced by Time-Varying Magnetic Fields

Guo

Qian

et al. 2022

Preprint

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In this work, an improved simulation model was proposed to assess the transmembrane potential (TMP) evolution on the cellular membrane exposed to timevarying magnetic fields (TMFs). Comparatively, we extended the research on TMP induced by TMF to the electroporation phenomenon by introducing the Smoluchowski function, thereby predicting the occurrence of electroporation. The simulation results based on our numerical model showed that with exposure to the sub-microsecond trapezoidal pulsed magnetic field (PMF), the pore density did not reach the conventional electroporation criterion(1014m-2 )

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“…Furthermore, a microfluidic device inside of a coil was used to concentrate Jurkat T‐lymphoblasts by using cDEP as stimulation. They reported a separation efficiency of 95% while using 1.96 × 10 12 V 2 /m 3 with 1–25 kHz [99]. In addition to the well documented contributions of DEP to leukemia cell manipulation, efforts have been made toward characterizing the electrical properties of HL‐60 cells in the presence of neutrophils by using a DEP spring.…”

Section: Electrokinetically‐driven Microfluidics For Cancer Cellsmentioning

confidence: 99%

A survey of electrokinetically‐driven microfluidics for cancer cells manipulation

et al. 2020

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Cancer is one of the leading causes of annual deaths worldwide, accounting for nearly 10 million deaths each year. Metastasis, the process by which cancer spreads across the patient's body, is the main cause of death in cancer patients. Because the rising trend observed in statistics of new cancer cases and cancer‐related deaths does not allow for an optimistic viewpoint on the future—in relation to this terrible disease—the scientific community has sought methods to enable early detection of cancer and prevent the apparition of metastatic tumors. One such method is known as liquid biopsy, wherein a sample is taken from a bodily fluid and analyzed for the presence of CTCs or other cancer biomarkers (e.g., growth factors). With this objective, interest is growing by year in electrokinetically‐driven microfluidics applied for the concentration, capture, filtration, transportation, and characterization of CTCs. Electrokinetic techniques—electrophoresis, dielectrophoresis, electrorotation, and electrothermal and EOF—have great potential for miniaturization and integration with electronic instrumentation for the development of point‐of‐care devices, which can become a tool for early cancer diagnostics and for the design of personalized therapeutics. In this contribution, we review the state of the art of electrokinetically‐driven microfluidics for cancer cells manipulation.

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Contactless dielectrophoretic manipulation of biological cells using pulsed magnetic fields

Cited by 11 publications

References 14 publications

Pulsed Electromagnetic Field Assisted in vitro Electroporation: A Pilot Study

Pulsed Electromagnetic Field Assisted in vitro Electroporation: A Pilot Study

Simulation study of Cell Transmembrane Potential and Electroporation Induced by Time-Varying Magnetic Fields

A survey of electrokinetically‐driven microfluidics for cancer cells manipulation

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