Content of phenolic substances in basidiomycetes

Chemical Analysis of the Lamella Walls of Agaricus bisporus Fruit Bodies

“…Lipids were extracted several times with ether after treatment of the walls with 6 N HCl for 4 h at room temperature and quantified gravimetrically. Ash content was evaluated by weighing before and after placing the walls in a chinaware crucible at 550°C for 2 h. Phenolic substance assay was performed by a colorimetric technique with the Folin-Ciocalteau reagent, as described by Del Signore and colleagues [11], including potassium pyrosulfite (K 2 S 2 O 5 ) as an inhibitor of the phenoloxidases. For identification of monosaccharides, the fractions were hydrolyzed either with 4 N H 2 SO 4 for 5 h at 100°C or 5 M trifluoroacetic acid (TFA) for 1 h at the same temperature in sealed evacuated ampoules.…”

Phenolic profile of seventeen Portuguese wild mushrooms

“…Mushrooms have developed chemical defence mechanisms (against insects and microorganisms) analogous to those in plants, such as the production of phenolic compounds. In fact, phenolic compounds have been shown to protect the plant cell wall during UV, salt, or pathogenic stress (Signore, Romeo, & Giaccio, 1997). Indeed, other authors (Ribeiro et al, 2007) reported the presence of caffeic, p-coumaric and ellagic acids in F. hepatica collected in the same region (Bragança, Portugal) in 2004.…”

Can consumption of antioxidant rich mushrooms extend longevity?: antioxidant activity of Pleurotus spp. and its effects on Mexican fruit flies’ (Anastrepha ludens) longevity

“…Essentially, every phenolic substance plays an important role of response and resistance to pathogenic and infective agents. Del Signore et al (1997) determined the phenol content of 75 macromycete carpophore samples. In the case of Pleurotus species, these authors determined values between 0.9 and 2.95 mg/ml of total phenols in an extract of 1 mg carpophore/ml.…”