Continental-scale spatio-temporal distribution of antibiotic resistance genes in coastal waters along coastline of China

Lü, Jian; Zhang, Yuxuan; Wu, Jun

doi:10.1016/j.chemosphere.2020.125908

Cited by 49 publications

(11 citation statements)

References 33 publications

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“…It has been confirmed that different estuaries demonstrated different seasonal trends in the ARGs abundance due to the fickle seasonal nature of pollutant discharge from the rivers and the various behaviors of estuarine sediment ARGs (Lu and Lu, 2020). Besides, previous research suggested that the temperature didn't have significant effects on the distribution of ARGs in coastal areas and it demonstrated that ARGs in coastal areas along the Bohai Sea were relatively abundant in winter (Lu et al, 2020a(Lu et al, , 2020b. The variation of ARGs as a result of environmental fluctuations was probably induced by multiple human activities to some extent.…”

Section: Absolute and Relative Abundances Of Arg Subtypesmentioning

confidence: 84%

“…The qPCR reaction was performed with 10.0 μL mixture (5.0 μL SYBR® Premix Ex Taq™ II, 0.4 μL forward primer, 0.4 μL reverse primer, 1.0 μL DNA template and 3.2 μL ddH 2 O) in triplicate. The qPCR amplified reactions were performed using Bio-Rad CFX384 system with the program as previous study (Lu et al, 2020a(Lu et al, , 2020b: 30 s at 95°C (denaturation), 40 cycles for 5 s at 95°C (denaturation), 30 s at annealing temperature and 40 s at 72°C (extension). The melting curve was performed with temperature in the range of 60°C to 95°C, and the melting curve was considered as specific amplification when it was detected with a single peak.…”

Section: Dna Extraction and Quantification Of Target Genesmentioning

confidence: 99%

“…RDA analysis between ARGs and environmental factors was conducted based on the absolute abundances of ARGs. Many studies have been focused on the influence of organic pollutants and nutrient substances on the distribution of ARGs (Lu et al, 2020a(Lu et al, , 2020b. The basic qualities of sediments (water content, grain size, TC and TN) and water (ammonia, nitrate, nitrite, phosphate and silicate) in corresponding aquatic environment were both measured and analyzed (Fig.…”

Section: Ordination Analysis For Different Sediment Samplesmentioning

confidence: 99%

“…However, extensive and improper use of antibiotics has led to the prevalence of antibiotic resistance (Zhang et al, 2015). A lot of investigations focused on the occurrence, propagation and dissemination of antibiotic resistance genes (ARGs) in wastewater and sludge in wastewater treatment plants (Lu et al, 2020a(Lu et al, , 2020bYin et al, 2019), livestock breeding (Jia et al, 2017), aquacultural systems (Wang et al, 2018) and landfill (Zhao et al, 2018), or water and sediments in rivers (Dang et al, 2017), estuaries (Zhu et al, 2017) and public parks (Wang et al, 2014), etc. All of these targets, especially the rivers and mariculture, contributed significantly to ARGs pollution in coastal areas (Zhang et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Occurrence and distribution of antibiotic resistance genes in sediments in a semi-enclosed continental shelf sea

Zhang

Lü

et al. 2020

Science of The Total Environment

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Pollution of ARGs in sediments from the Bohai Sea areas was firstly investigated. • ARGs abundances varied over 3 orders of magnitude in different sediments. • ARGs were more abundant in the Laizhou Bay and eastern of central sea basin. • Abundance of sulfonamides resistance genes ranged from 5.7 × 10 4 to 1.8 × 10 7 copies/g. • Potential host bacteria of ARGs were Lysobacter, Thiobacillus, a n d Defluviimonas.

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Section: Absolute and Relative Abundances Of Arg Subtypesmentioning

confidence: 84%

Section: Dna Extraction and Quantification Of Target Genesmentioning

confidence: 99%

Section: Ordination Analysis For Different Sediment Samplesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Occurrence and distribution of antibiotic resistance genes in sediments in a semi-enclosed continental shelf sea

Zhang

Lü

et al. 2020

Science of The Total Environment

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“…In the coastal environment all over the world, the emergence of antibiotics and the spread of antibiotic resistance genes (ARGs) is believed to be promoted under anthropogenic impacts, such as wastewater disposal and mariculture ( Niu et al, 2016 ; Zhang et al, 2018 ; Lu et al, 2020 ). In China, for example, high concentrations of antibiotic residues and ARGs are frequently detected in the seawater and sediments along the coastline ( Zhang et al, 2013 ; Chen et al, 2017 ; Zhu et al, 2017 ; Chen J. et al, 2019 ; Li et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Evaluating the Potential Antibiotic Resistance Status in Environment Based on the Trait of Microbial Community

Huang

et al. 2020

Front. Microbiol.

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The overuse of antibiotics has promoted the propagation and dissemination of antibiotic resistance genes (ARGs) in environment. Due to the dense human population and intensive activities in coastal areas, the health risk of ARGs in coastal environment is becoming a severe problem. To date, there still lacks of a quantitative method to assess properly the gross antibiotic resistance at microbial community level. Here, we collected sediment samples from Hangzhou Bay (HB), Taizhou Bay (TB), and Xiangshan Bay (XB) of the East China Sea for community-level ARGs analysis. Based on the 16S rRNA genes and predictive metagenomics, we predicted the composition of intrinsic ARGs (piARGs) and some related functional groups. Firstly, a total of 40 piARG subtypes, belonging to nine drug classes and five resistance mechanisms, were obtained, among which the piARGs encoding multidrug efflux pumps were the most dominant in the three bays. Secondly, XB had higher relative abundances of piARGs and pathogens than the other two bays, which posed higher potential health risk and implied the heavier impact of long-term maricultural activities in this bay. Thirdly, the co-occurrence network analysis identified that there were more connections between piARGs and some potential pathogenic bacteria. Several piARG subtypes (e.g., tetA , aacA , aacC , and aadK ) distributed widely in the microbial communities. And finally, the microbial diversity correlated negatively with the relative abundance of piARGs. Oil, salinity, and arsenic had significant effects on the variations of piARGs and potential pathogenic bacteria. The abundance-weighted average ribosomal RNA operon ( rrn ) copy number of microbial communities could be regarded as an indicator to evaluate the antibiotic resistance status. In conclusion, this study provides a new insight on how to evaluate antibiotic resistance status and their potential risk in environment based on a quantitative analysis of microbial communities.

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Organics and Nutrient Transformation in a Microbial Fuel Cell Influenced by Antibiotics

Tariq

Wang

Sehar

et al. 2023

CLEAN Soil Air Water

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The present research focuses on the effect of the highly toxic and broad‐spectrum bacteriostatic antibiotic pollutant, tetracycline (TC) as a sole electron donor and co‐electron donor on the operational efficacy of a microbial fuel cell (MFC) in terms of nutrient and pollutants removal. It is observed that the removal efficiency of the MFC increases by the gradual increase in TC concentration (5–30 mg L−1). The maximum removal efficiency (59%), voltage production (372 mV), power density (81.4 mW m−2), and current density (232.5 mA m−2) are achieved in the MFC with 25 mg L−1 of TC. Moreover, the influential role of TC as a co‐substrate is also investigated using three substrates viz, acetic acid, sucrose, and albumin. The current results suggest that chemical oxygen demand (COD) removal efficiency, coulombic efficiency, and voltage output of each substrate are inversely proportional to the amount of TC and are in the order of acetic acid > sucrose > albumin. The nutrient removal efficiency of the MFC decreases with an increase in TC concentration, i.e., when TC is increased from 10 to 50 mg L−1, the nitrogen removal efficiency decreases from 78% to 38%, the sulfur removal efficiency decreases from 79% to 32%, and the phosphorous removal efficiency decreases from 72% to 24%. The results suggest that an MFC can be upgraded for large‐scale biological processes for antibiotic pollutant removal. Moreover, the adverse effect of TC in power generation of an MFC can serve as a biosensor for biological wastewater treatment processes to detect the antibiotic toxicity.

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Continental-scale spatio-temporal distribution of antibiotic resistance genes in coastal waters along coastline of China

Cited by 49 publications

References 33 publications

Occurrence and distribution of antibiotic resistance genes in sediments in a semi-enclosed continental shelf sea

Occurrence and distribution of antibiotic resistance genes in sediments in a semi-enclosed continental shelf sea

Evaluating the Potential Antibiotic Resistance Status in Environment Based on the Trait of Microbial Community

Organics and Nutrient Transformation in a Microbial Fuel Cell Influenced by Antibiotics

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