Continuous Changes in Triacylglycerol Molecular Species Composition by Fatty Acids in Porcine Adipocytes

Suzuki, Sayoko; Ishikawa, Shin‐ichi; Arihara, Keizou; Itoh, Makoto

doi:10.1271/bbb.70674

Cited by 1 publication

(1 citation statement)

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“…In addition, the role of some candidate genes in relation to the pig fat development will also make it possible to demonstrate by using PSPA cells with gene transfer technique (Taniguchi et al 2014). Further, this simple culture system allows us to readily monitor adipose development under conditions of controlled supplements and substrates to cultural medium easily to determine the effect of some diets for the possibility to regulate pig fat (Suzuki et al 2008, Irie et al 2012. However, over the past two decades, the view of adipose tissue has undergone a dramatic change from an inert excess energy reservoir to a dynamic endocrine organ (Wang et al 2008, Deng & Scherer 2010.…”

Section: Future Perspectivesmentioning

confidence: 99%

Characterization of PSPA Cell Differentiation,an Established Pig Preadipocyte Cell Line as an In Vitro Model for Pig Fat Development

Nakajima

2015

JARQ

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We have established a clonal porcine subcutaneous preadipocyte cell line for the first time. This cell line, named PSPA, shows a fibroblast-like phenotype and keeps on growing under growth medium even after reaching confluence. Adipose conversion occurs only when confluent cultures are stimulated with insulin, dexamethasone, biotin, pantothenate, and octanoate. Following exposure to this differentiation medium, growth was arrested immediately without mitotic expansion and PSPA cells exhibited a marked increase in intracellular lipid accumulation accompanied by changes in gene expressions directly related to fat metabolism and also in extracellular environments. Since PSPA cells were established, it has become possible to end the discussion over whether there is species specificity in the adipose conversion between pig and mouse by comparing PSPA cells and 3T3-L1 cells, the most widely studied preadipocyte cell line. Consequently, we demonstrated that responsiveness to inducers and fatty acid composition was unequal, despite being treated with the same media. Thus, species-specific differences indeed exist in adipogenesis between these two, meaning that PSPA cells would be an in vitro model specialized to improve understanding of pig adipocyte growth and differentiation in controlling the carcass fat content of pigs.

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Section: Future Perspectivesmentioning

confidence: 99%

Characterization of PSPA Cell Differentiation,an Established Pig Preadipocyte Cell Line as an In Vitro Model for Pig Fat Development

Nakajima

2015

JARQ

View full text Add to dashboard Cite

show abstract

Continuous Changes in Triacylglycerol Molecular Species Composition by Fatty Acids in Porcine Adipocytes

Cited by 1 publication

References 36 publications

Characterization of PSPA Cell Differentiation,an Established Pig Preadipocyte Cell Line as an In Vitro Model for Pig Fat Development

Characterization of PSPA Cell Differentiation,an Established Pig Preadipocyte Cell Line as an In Vitro Model for Pig Fat Development

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