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The compositions of fatty acid and molecular species of triacylglycerol of subcutaneous fat of fattened Japanese Black, Japanese Shorthorn and Holstein steers were determined by gas chromatography.There were significant differences in heptadecanoic acid concentration among three breeds, and in six acid (myristic, myristoleic, palmitic, palmitoleic, anteiso-heptadecanoic and stearic acids) concentrations between two breeds of three breeds. The relationship between palmitic and oleic acid concentrations was negative.The major molecular species of subcutaneous triacylglycerol were myristoyl (M)-palmitoyl (P)-oleoyl (O)-glycerol (MPO), PPO, PPalmitoleoyl (Po)-O-glycerol ([PPoO]), P-stearoyl (S)-O-glycerol (PSO) and POO. POO had the highest concentration in these molecular species of three breeds. Japanese Black had higher concentrations of PSO and SOL than those of Holstein. Relationships between POL and POO, and PPO and POO were positive and negative, respectively.In order to analyze the association of total lipid content of M longissimus thoracis and molecular species composition of triacylglycerol, multiple regression analysis was carried out. In the linear multiple regression analysis, the regression equation of Japanese Black had a good multiple correlation coefficient (0.8110). In discriminant analysis of samples separated according to meat quality score of carcass, the percentage of discrimination were 100% for each group of Japanese Black and Japanese Shorthorn.

The compositions of molecular species of triacylglycerol of subcutaneous fat biopsies taken from fattening spaiers and steers of Japanese Black were determined.There were no differences in the molecular species concentrations of triacylglycerols between spaiers and steers at 13 or 30 months of age, however the molecular species compositions of triacylglycerols of the 13 months of age were significantly different from those of the 30 months of age. The major molecular species of subcutaneous triacylglycerol at 13 months of age were [dipalmitoyland POO, and at 30 months of age were PPO and POO. POO had the highest concentration in these molecular species. POO, POL and SOL concentrations of subcutaneous triacylglycerols at 30 months of age were higher than those of 13 months of age.

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acid and molecular species compositions of triacylglycerols among three kinds of muscles. The major fatty acids of intramuscular triacylglycerols were palmitic acid (C16:0), stearic acid (C8:0) and oleic acid (C18:1) where C18:1 had the highest concentration. The major molecular species of intramuscular triacylglycerols were dipalmitoyl (PP)-oleoyl (O)-glycerol (PPO) and P-stearoyl (S)-O-glycerol (PSO), POO and P-O-linoleoyl (L)-glycerol (POL). POO had the highest concentration in these molecular species.