Continuous citric acid production by immobilized aspergillus niger: reactor performance and fermentation kinetics

Vaija, J.; Linko, P.

doi:10.1016/0304-5102(86)85030-1

Cited by 20 publications

(7 citation statements)

References 25 publications

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“…Correspondence to: H. Kautola niger cells immobilized on calcium alginate gel were later studied in a greater detail by Eikmeier and Rehm (1984), Vaija and Linko (1986), and Honecker et al (1989). Eikmeier and Rehm (1987) reported as much as a 20°7o loss in stability of the initial strength during precultivation of the cells and acid production.…”

Section: Introductionmentioning

confidence: 99%

Studies on citric acid production with immobilized Yarrowia lipolytica in repeated batch and continuous air-lift bioreactors

Rymowicz¹,

Kautola

Wojtatowicz³

et al. 1993

Appl Microbiol Biotechnol

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Citric acid was produced from glucose in repeated-batch shake-flask and continuous air-lift cultivations by calcium-alginate-immobilized Yarrowia lipolytica A-101 yeast. The medium composition was systematically studied in a batch system by using experimental design and empiric modelling. The highest citric acid product concentration of 39 g/1 was reached with a medium containing 150 g/1 of glucose, 0.105 g/1 of potassium dihydrogen phosphate, 0.84g/I of magnesium sulphate and 21 mg/1 of copper sulphate (5.2 rag/1 of copper). The results were further improved by hardening the alginate carrier beads with glutaraldehyde, and by activation of the immobilized biocatalyst in a nutrient solution. In continuous air-lift bioreactors with varying height-to-diameter ratio the highest productivity of 350 rag/1 per hour with a dilution rate of 0.023 1/h and a citric acid product concentration of 12 g/1 was reached with a ratio of 3.

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Section: Introductionmentioning

confidence: 99%

Studies on citric acid production with immobilized Yarrowia lipolytica in repeated batch and continuous air-lift bioreactors

Rymowicz¹,

Kautola

Wojtatowicz³

et al. 1993

Appl Microbiol Biotechnol

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“…It is possible that part of the citric acid produced at the outside is consumed by the biomass that is located in the insides of the bead. In order to overcome this,using smaller sized beads or using pure oxygen as the aeration medium had been proposed (2).…”

Section: Resultsmentioning

confidence: 99%

“…It has been found both theoretically experimentally that the smaller is the bead the more suitable it will be for citric acid 9 production. However, the difficulties of making smaller beads and the mechanical properties of small beads impose limitations on such a proces (1, 2,3). In this work we tried a new immobilization method for obtaining smaller beads which are mechanically more durable and could also be valuable in storing microorg.anisms in the immobilized form.…”

Section: Introductionmentioning

confidence: 99%

Production of citric acid by immobilized dried reactivatedAspergillus niger

Hamamcı

Hang

1989

Biotechnol Tech

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Immobilization followed by drying was tried as a new technique for obtaining small beads which might be more suitable for industrial fermentations and for the storage of the immobilized microorganisms. Aspergillus niger NRRL 2270 was used to produce citric acid in free, immobilized, and immobilized dried reactivated (IDR) forms. The productivity based on the bead volume used increased several folds with the use of IDR cells although the absolute level of citric acid did not increase.

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“…A detailed study on the engineering aspects was carded out. Citric acid production has also been reported in a laboratory scale with A. niger cells immobilized on calcium alginate gel (Vaija et al 1982;Eikmeier and Rehm 1984;Vaija and Linko 1986;Honecker et al 1989), polyacrylamide gel (Horitsu et al 1985), and on polyurethane foam (Lee et al 1989). The early work on citric acid production both with polyacrylamide-gel-entrapped Candida (syn.…”

Section: Introductionmentioning

confidence: 99%

Production of citric acid with immobilized Yarrowia lipolytica

Kautola

Rymowicz

Linko

et al. 1991

Appl Microbiol Biotechnol

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Citric acid was produced with immobilized Yarrowia lipolytiea yeast in repeated batch-shake-flask and air-lift fermentations. In active and passive immobilization methods calcium alginate, tc-carrageenan, polyurethane gel, nylon web and polyurethane foams were tested as carriers in repeated-batch fermentations. The highest citric acid productivity of 155 mg 1-1 h -1 was reached with alginate-bead-immobilized cells in the first batch. A decrease in bead diameter from 5-6 m m to 2-3 m m increased the volumetric citric acid productivity threefold. In an air-lift bioreactor the highest citric acid productivity of 120 mg1-1 h -1 with a product concentration of 16.4 g 1-1 was obtained with cells immobilized in tc-carrageenan beads.

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Continuous citric acid production by immobilized aspergillus niger: reactor performance and fermentation kinetics

Cited by 20 publications

References 25 publications

Studies on citric acid production with immobilized Yarrowia lipolytica in repeated batch and continuous air-lift bioreactors

Studies on citric acid production with immobilized Yarrowia lipolytica in repeated batch and continuous air-lift bioreactors

Production of citric acid by immobilized dried reactivatedAspergillus niger

Production of citric acid with immobilized Yarrowia lipolytica

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