Continuous culture of <i>Candida lipolytica</i> on <i>n</i>‐hexadecane

The interest of Mallee and Blanch1 in our workZ is appreciated. The quantity of nitrogen added for pH control via NH,OH addition approximately equals that incorporated into biomass when no acidic products are produced. For hydrocarbon fermentation, for example, Prokop et al.3 reported that they added 2.09 ml 10% NaOH/g cell dry weight produced for pH control or about 1 equivalent (equiv)/equiv nitrogen incorporated into biomass based on 7% nitrogen in biomass.

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Predominant role of hydrocarbon solubilization in the microbial uptake of hydrocarbons

Reddy

Singh

Roy

et al. 1982

Biotech & Bioengineering

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Using EDTA and proteolytic enzymes to suppress hydrocarbon solubilization, direct evidence is presented in support of the mechanism of liquid hydrocarbon uptake by microbial cells predominantly from the solubilized or accommodated substrate. EDTA (2-5mM) strongly inhibited growth of three yeast species and one bacterial species on n-hexadecane and the inhibition was removed by surfactant-emulsified and surfactant-solubilized alkane and also by excess addition of Ca(2+). EDTA had no inhibitory effect on the growth of the organisms on soluble substrates such as sodium acetate and nutrient broth or on n-pentane, a volatile alkane which was primarily transported by diffusion from gas phase. EDTA was shown to have no significant effect on the adsorption of cells on alkane drops. EDTA inhibition of growth was considered to be due to suppression of alkane solubilization, brought about by the solubilizing factor(s) produced by cells. It was shown that this chelating agent did not inhibit the growth of yeast on solubilized alkane but strongly inhibited its growth on alkane drops. It was demonstrated that adherent capacity of microbial cell to oil phase was closely related to the state of hydrocarbon emulsification and had no relationship to the ability of organisms to grow on hydrocarbon. Certain proteolytic enzymes inhibited the growth of yeast on alkane, presumably by digesting the alkane solubilizing protein, but not on glucose, and the inhibition was removed by a supply of surfactant-emulsified and surfactant-solubilized alkane. Specific solubilization of various hydrocarbon types during growth of the prokaryotic bacterial strain was demonstrated. The specific solubilization of hydrocarbon was strongly inhibited strain was demonstrated. The specific solubilization of hydrocarbon was strongly inhibited by EDTA, and the inhibition was removed by excess Ca(2+). It was concluded that specific solubilization of hydrocarbons is an important mechanism in the microbial uptake of hydrocarbons.

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Continuous culture of Candida lipolytica on n‐hexadecane

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Predominant role of hydrocarbon solubilization in the microbial uptake of hydrocarbons

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