Continuous degradation of 3-chloroaniline by calcium-alginate-entrapped cells of Pseudomonas acidovorans CA28: influence of additional substrates

Ferschl, Andreas; Loidl, Michael; Ditzelmüller, Günther; Hinteregger, Christel; Streichsbier, Franz

doi:10.1007/bf00169766

Cited by 31 publications

(18 citation statements)

References 19 publications

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“…Exponentially growing cells were harvested by centrifuging the culture broth at 6,000 rpm for 20 min and washing with a 0.9% (wlv) NaCI solution. The washed cells (1.4g dry wt./I) were immobilized in gel beads of calcium alginate, as described in a previous report (Ferschl et al, 1991). Culture conditions.…”

Section: Methodsmentioning

confidence: 99%

Biodegradation of the mixture of 2,4,6-trichlorophenol, 4-chlorophenol, and phenol by a defined mixed culture.

Bae

Lee

1997

J. Gen. Appl. Microbiol.

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KoreaTwo new strains, Pseudomonas sp. TCP114 degrading 2, 4, 6-trichlorophenol (TCP) and Arthrobacter sp. CPR706 degrading 4-chlorophenol (4-CP), were isolated through a selective enrichment procedure. Both strains could also degrade phenol. The degradability of one component by a pure culture was strongly affected by the presence of other compounds in the medium. For example, when all three components (TCP, 4-CP, and phenol) were present in the medium, a pure culture of CPR706 could not degrade any of the components present. This restriction on degradability could be overcome by employing a defined mixed culture of the two strains. The mixed culture could degrade all three components in the mixture through cooperative activity. It was also demonstrated that the mixed culture could be immobilized by using calcium alginate for the semi-continuous degradation of the three-component mixture. Immobilization not only accelerates the degradation rate, but also enables reuse of the cell mass several times without losing the cells' degrading capabilities.

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Section: Methodsmentioning

confidence: 99%

Biodegradation of the mixture of 2,4,6-trichlorophenol, 4-chlorophenol, and phenol by a defined mixed culture.

Bae

Lee

1997

J. Gen. Appl. Microbiol.

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“…Another aniline-degrading pseudomonad, however, was shown to utilize aniline and other organic substrates simultaneously (9). In other studies, it also became apparent that catabolite repression of catabolic pathways for xenobiotic compounds is not as common as could be expected; 3-phenoxybenzoate metabolism by a strain of Pseudomonas delafieldii was shown to be nonsusceptible to catabolite repression (22), as was the degradation of 3-chloroaniline by Pseudomonas acidovorans (7). Release of catabolite repression of catabolic enzymes in a chemostat culture limited by a potentially repressing substrate, as observed in this study, is a generally occurring phenomenon (8,16).…”

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confidence: 99%

Inducibility of the TOL catabolic pathway in Pseudomonas putida (pWW0) growing on succinate in continuous culture: evidence of carbon catabolite repression control

et al. 1994

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The TOL catabolic genes in Pseudomonas putida(pWWO) are clustered in the upper operon, encoding enzymes for the conversion of toluene and xylenes to benzoate and toluates, and the meta-cleavage operon, encoding enzymes for the conversion of the benzoate and toluates to tricarboxylic acid cycle intermediates. In this study, it was shown that cells growing in a chemostat under succinate growth-limiting conditions express both the upper and meta-cleavage pathways in response to o-xylene, a nonmetabolizable effector of the XylR regulatory protein. The dilution rate maintained in the succinate-limited chemostat cultures influenced the synthesis levels of TOL pathway enzymes, their steady-state levels, and their turnover rates. Cells growing in the presence of nonlimiting concentrations of succinate in continuous culture did not express pathway enzymes in response to the addition of o-xylene, which was due to a blockage at the transcriptional level. Expression of the meta-cleavage pathway in response to 2,3-dimethylbenzoate, a nonmetabolizable elector of the XylS regulatory protein, was 93% lower in cultures exposed to succinate at nonlimiting concentrations than in the succinate-limited chemostats. The mRNA level of xylS during nonlimited growth on succinate was very low compared with that in succinate-limited cultures, suggesting that suppression of expression of the metacleavage pathway is regulated mainly by the level of the XylS regulator.Many soil bacteria are known to be capable of mineralizing aromatic compounds. The most extensively studied organism in this respect is Pseudomonas putida(pWWO), which can utilize toluene, m-and p-xylene, pseudocumene, and mi-ethyltoluene as sole sources of carbon and energy. The genetic information for the transformation of these aromatics to central metabolites is harbored by the transmissible TOL plasmid pWWO (3). Figure 1 shows the genetic organization of the catabolic operons. Expression of the structural genes on

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“…The overall COD removal by the two stages exceeded 85%. A continuous suspended draft-tube sparged concentric-tube airlift bioreactor was applied for 3-chloroaniline degradation [65]. The airlift was applied as a system of aerators for treating industrial wastes in an aeration-flotation tank using a system of cylindrical draft-tubes.…”

Section: Modifications Of the Activated-sludge Processesmentioning

confidence: 99%

“…In actual operation, most of the airlift bioreactors lie somewhere between these two extremes and the deviations from ideal flow can have a variety of causes [55,[57][58][59]: incomplete radial mixing, liquid back-mixing, existence of stagnant regions and channelling. Therefore, airlift bioreactors can be considered as plug flow, with superimposed axial dispersion, according to the following equation [36,44,47,[54][55][56][57][58][59][60][61][62][63][64][65][66][67][68][69][70][71] vated cell concentrations…”

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confidence: 99%

Process engineering in biological aerobic waste‐water treatment

Macoveanu

1999

Acta Biotechnologica

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A non-comprehensive review of several technical developments in the field of aerobic biological waste-water treatment engineering is camed out, eonsidering the active role the engineers have to play in this field. This paper brings together conventional and advanced problems in the field of aerobic biological waste-water treatment. Such an overview of biological waste-water treatment also precedes comments on some important aspects concerning the microorganisms responsible for waste-water treatment as well as considerations of the application of fundamentals and kinetics to the analysis of the biological processes used most commonly for aerobic biological waste-water treatment.A survey of the development of the biological activated-sludge process and some modifications are given. Some problems implied in the conventional activated-sludge waste-water treatment are analyzed, considering conventional processes and bioreactor models (the continuous stirred-tank reactor model and the plug-flow reactor models of the activated-sludge process) as well as aerated lagoons. Further, modifications of the activated-sludge process are presented. These include additional details on the bioreactor progress and applications. with emphasis on aspects concerning airlift bioreactors and their variants, deep-shaft bioreactors and reciprocating jet bioreactors which are considered as the third generation of bioreactors owing to their important advantages in design, operation and performance in waste-water treatment. Sequencing-batch reactors and aerobic digestion processes, including conventional aerobic digestion, high-purity oxygen digestion, thermophilic aerobic digestion and cryophylic aerobic digestion are also reviewed. Finally, some aspects regarding the operational factors that are involved in the selection of the reactor type are included.

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Continuous degradation of 3-chloroaniline by calcium-alginate-entrapped cells of Pseudomonas acidovorans CA28: influence of additional substrates

Cited by 31 publications

References 19 publications

Biodegradation of the mixture of 2,4,6-trichlorophenol, 4-chlorophenol, and phenol by a defined mixed culture.

Biodegradation of the mixture of 2,4,6-trichlorophenol, 4-chlorophenol, and phenol by a defined mixed culture.

Inducibility of the TOL catabolic pathway in Pseudomonas putida (pWW0) growing on succinate in continuous culture: evidence of carbon catabolite repression control

Process engineering in biological aerobic waste‐water treatment

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