Continuous electrophoretic separations in narrow channels with postchannel derivatization and laser-induced fluorescence detection

MacTaylor, Christine E.; Ewing, Andrew G.

doi:10.1002/(sici)1520-667x(2000)12:5<279::aid-mcs1>3.0.co;2-k

Cited by 5 publications

(7 citation statements)

References 19 publications

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“…In that approach, the CZE outlet capillary end is moved across the entrance of the gel channel, thereby preserving completely both separations. The concept of combining a single capillary, which is used for sampling, and depositing the analytes across the entrance of a rectangular channel stems from the work of Ewing's group (173)(174)(175)(176). It was developed with the goal of continuous monitoring of biological systems and was applied to a variety of situations, including the detection of neurotransmitters, the kinetics of small molecules and to monitor the chemical environment around a single cell (175,177).…”

Section: Multidimensional Separationsmentioning

confidence: 99%

Capillary Electrophoretic Separations

Thormann

2011

Methods of Biochemical Analysis

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Section: Multidimensional Separationsmentioning

confidence: 99%

Capillary Electrophoretic Separations

Thormann

2011

Methods of Biochemical Analysis

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“…At present, there is interest in single cell or subcellular analysis and therefore in developing the appropriate instrumentation and methods [170][171][172]. CE miniaturization will continue either through reducing column dimensions or placing entire electrophoresis systems on planar microfluidic chips [165,[173][174][175][176][177][178][179]. The electrophoretic separations in microchips significantly faster than the conventional separations, will have an effect especially in DNA separations [180].…”

Section: Miniaturization Of Ce Systemsmentioning

confidence: 99%

Biomedical applications of capillary electrophoresis with laser‐induced fluorescence detection

Páez

Hernández

2001

Biopharm & Drug Disp

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Capillary electrophoresis (CE) is a high-efficiency analytical technique that has had a great impact as a tool in biomedical research, clinical and forensic practice in the last ten years. Only in one of the applications, the DNA analysis, it has had an explosive exponential growth in the last few years. This impact is expressed in an enormous amount of CE articles and many reviews. The CE advantages with respect to other analytical techniques: the required very small sample volume, rapid analysis, great resolution power and low costs, have made this technique ideal for the analysis of a numerous endogenous and exogenous substances present in biological fluids. The different modes of CE have been coupled to different detection techniques such as UV-absorbance, electrochemical, mass spectrometry and laser-induced fluorescence detection (LIFD) to detect different nature and molecular size separated analytes. This review focuses mostly on the applications of CE-LIFD, to measure drugs and endogenous neuroactive substances such as amino acids and monoamines, especially in microdialysis samples from experimental animals and humans. CE-LIFD trends are discussed: automated faster analysis with capillary array systems, resolution power improvement, higher detection sensitivity, and CE systems miniaturization for extremely small sample volume, in order to make CE easier and affordable to the lab bench or the clinical bed.

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“…The LOD for CA is about absorbance detection that is more universal but less 31 pM when injected at 15 kV for 600 s, which is sensitive. To perform CE-LIF, proteins must be about three orders of magnitude of sensitivity enlabeled with suitable fluorephores such as fluorescein hancement compared to that injected at 1 kV for 10 and its derivatives or contain tryptophan, tyrosine s. Although this new method provides advantages and phenylalanine residues [19][20][21]. Generally, a over most conventional methods [24][25][26][27][28] in CE, limit of detection (LOD) at the nM level is easily including no need to fill the capillary with polymer achieved under mild conditions, such as low ionic solution prior to analysis; high stacking efficiency; strengths [22].…”

Section: Introductionmentioning

confidence: 99%