Continuous-flow chemiluminescence determination of some corticosteroids

Abstract

A continuous-flow method for the determination of cortisone, hydrocortisone, prednisolone and methylprednisolone in the range 0.100-1.00 pg ml-1 and of dexamethasone and betamethasone in the range 0.500-5.00 pg ml-1 is described. The procedure is based on the action of each corticosteroid as a sensitiser on the chemiluminescent oxidation of sulphite by cerium(lV). The analysis is automated, requires no sample pre-treatment and samples can be analysed at a rate of 40 per hour with a relative error of <6%. The method was evaluated by carrying out recovery experiments and by analysing commercial formulations. Results agreed well with those given by an official method.

Chemiluminescent flow-through sensor for automated dissolution testing of analgin tablets using manganese dioxide as oxidate

“…The influences of different acidic media on the CL intensity were studied ( Table 1). The results showed the sulfuric acid was the optimum medium, the same with the CL system involving Ce(IV) or MnO 4 − [26,27,[34][35][36]. Furthermore, the effect of H 2 SO 4 concentration was studied (Fig.…”

Determination of enalapril maleate and atenolol in their pharmaceutical products and in biological fluids by flow‐injection chemiluminescence

“…[51][52][53] The emission has been attributed to the formation of excited sulfur dioxide molecules which radiate during de-excitation. [54] The aim of the present work is to develop a sensitive and accurate flow injection CL method for the determination of enalapril maleate and atenolol in pharmaceutical preparations and biological fluids.…”

Recent Advances in the Analysis of Steroid Hormones and Related Drugs

“…The sensitising effect of corticosteroids on the chemoluminogenic reaction between sulfite and cerium(IV) was exploited for their flow-injection (FIA) determination in pharmaceutical formulations. 52,53 A luminol chemiluminescence determination for the same purpose is also described. 54…”