Continuous flow generation of magnetoliposomes in a low-cost portable microfluidic platform

Conde, Alvaro J.; Batalla, M.; Cerda, Belén; Mykhaylyk, Olga; Plank, Christian; Podhajcer, Osvaldo L.; Cabaleiro, Juan Martin; Madrid, Rossana E.; Policastro, L.

doi:10.1039/c4lc00839a

Cited by 14 publications

(17 citation statements)

References 29 publications

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“…This seal puts both valves in a closed state that avoids unwanted leaking and is also closely related to the working mechanism of outlet check All the PMMA layers of the pump were fabricated computer numerical control (CNC) micromilling following a procedure previously described. 21 (Fig. 1c).…”

Section: Communicationmentioning

confidence: 93%

“…21 Briefly, the pump layers were gently cleaned to remove debris, sonicated in isopropyl alcohol for 2 min, rinsed with deionized water and finally b The PDMS diaphragms were fitted in their corresponding O ring gland just after the bonding faces of the layers were exposed to UV light (250 W quartz mercury lamp) for 1 min. The layers were stacked, fitted in an alignment fra subsequently bonded between glass plates in a bonding press (Shimeq, Argentina) at 80 °C for 15 min with an applied pressure of 2 MPa.…”

Section: Communicationmentioning

confidence: 99%

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A polymer chip-integrable piezoelectric micropump with low backpressure dependence

Conde

Bianchetti²,

Veiras³

et al. 2015

RSC Adv.

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Section: Communicationmentioning

confidence: 93%

Section: Communicationmentioning

confidence: 99%

A polymer chip-integrable piezoelectric micropump with low backpressure dependence

Conde

Bianchetti²,

Veiras³

et al. 2015

RSC Adv.

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“…The control of flow in microfluidic networks is crucial for cell sorting, cell collection, flow mixing, cell adhesion and culture, droplet manipulation and flow driving 1 . Moreover, the flow rate needs to be accurately quantified to determine the concentration of cells 2 , and production of hollow microspheres 3 , droplets 4 , liposomes 5 , and chitosan microfibers 6 . A slight change in flow rate may lead to a size variation in the products.…”

Section: Introductionmentioning

confidence: 99%

Noncontact and Nonintrusive Microwave-Microfluidic Flow Sensor for Energy and Biomedical Engineering

Zarifi

Saberi

Hejazi

et al. 2018

Sci Rep

155

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A novel flow sensor is presented to measure the flow rate within microchannels in a real-time, noncontact and nonintrusive manner. The microfluidic device is made of a fluidic microchannel sealed with a thin polymer layer interfacing the fluidics and microwave electronics. Deformation of the thin circular membrane alters the permittivity and conductivity over the sensitive zone of the microwave resonator device and enables high-resolution detection of flow rate in microfluidic channels using noncontact microwave as a standalone system. The flow sensor has the linear response in the range of 0-150 µl/min for the optimal sensor performance. The highest sensitivity is detected to be 0.5 µl/min for the membrane with the diameter of 3 mm and the thickness of 100 µm. The sensor is reproducible with the error of 0.1% for the flow rate of 10 µl/min. Furthermore, the sensor functioned very stable for 20 hrs performance within the cell culture incubator in 37 °C and 5% CO 2 environment for detecting the flow rate of the culture medium. This sensor does not need any contact with the liquid and is highly compatible with several applications in energy and biomedical engineering, and particularly for microfluidic-based lab-on-chips, micro-bioreactors and organ-on-chips platforms.Microfluidic techniques have been extensively used for efficient manipulation of fluid flow in microscale for biomedical research and analytical chemistry. The control of flow in microfluidic networks is crucial for cell sorting, cell collection, flow mixing, cell adhesion and culture, droplet manipulation and flow driving 1 . Moreover, the flow rate needs to be accurately quantified to determine the concentration of cells 2 , and production of hollow microspheres . A slight change in flow rate may lead to a size variation in the products. To precisely handle fluids at the microscale, the real-time detection of flow rate in microfluidic environment is essential and urgently needed though challenging.Organ-on-a-chip (OOC) technology, in particular, aims to build biomimetic in vitro physiological micro-organs to compliment animal models in biological systems and benefit the pharmaceutical industry for drug discovery 7,8 . Many groups including ours have developed OOC platforms made of microbioreactors and integrated sensors for long-term and real-time monitoring the microenvironment, screening the status of miniaturized organs, and characterizing the response of micro-tissues to drugs [9][10][11][12][13] . The real-time measurement of heat transfer 14 , differential pressure 15 , pH and oxygen 11 and biomarkers 10 are central to biomimetic performance of OOC systems. Miniaturized biosensors provide favorable features like low-cost reagents consumption, decreased processing time, reduced sample volume, laminar flow to cells, parallel detection for multiple samples as well as portability 12,13,16 . However, the OOC systems still need on-chip integrated flow sensors compatible with their fabrication processes and functions 17. The OOC platforms require the...

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“…size, polydispersity, etc., due to the process’s ability to accurately control process parameters and thus enables efficient, continuous and tunable mixing30313233343536.…”

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confidence: 99%

A Microfluidic Platform to design crosslinked Hyaluronic Acid Nanoparticles (cHANPs) for enhanced MRI

Russo

Bevilacqua

Netti

et al. 2016

Sci Rep

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Recent advancements in imaging diagnostics have focused on the use of nanostructures that entrap Magnetic Resonance Imaging (MRI) Contrast Agents (CAs), without the need to chemically modify the clinically approved compounds. Nevertheless, the exploitation of microfluidic platforms for their controlled and continuous production is still missing. Here, a microfluidic platform is used to synthesize crosslinked Hyaluronic Acid NanoParticles (cHANPs) in which a clinically relevant MRI-CAs, gadolinium diethylenetriamine penta-acetic acid (Gd-DTPA), is entrapped. This microfluidic process facilitates a high degree of control over particle synthesis, enabling the production of monodisperse particles as small as 35 nm. Furthermore, the interference of Gd-DTPA during polymer precipitation is overcome by finely tuning process parameters and leveraging the use of hydrophilic-lipophilic balance (HLB) of surfactants and pH conditions. For both production strategies proposed to design Gd-loaded cHANPs, a boosting of the relaxation rate T1 is observed since a T1 of 1562 is achieved with a 10 μM of Gd-loaded cHANPs while a similar value is reached with 100 μM of the relevant clinical Gd-DTPA in solution. The advanced microfluidic platform to synthesize intravascularly-injectable and completely biocompatible hydrogel nanoparticles entrapping clinically approved CAs enables the implementation of straightforward and scalable strategies in diagnostics and therapy applications.

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Continuous flow generation of magnetoliposomes in a low-cost portable microfluidic platform

Cited by 14 publications

References 29 publications

A polymer chip-integrable piezoelectric micropump with low backpressure dependence

A polymer chip-integrable piezoelectric micropump with low backpressure dependence

Noncontact and Nonintrusive Microwave-Microfluidic Flow Sensor for Energy and Biomedical Engineering

A Microfluidic Platform to design crosslinked Hyaluronic Acid Nanoparticles (cHANPs) for enhanced MRI

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