Continuous-flow, support-free, electrophoretic separation in thin layers: towards large-scale operation

Retention in continuous two-dimensional thermal field-flow fractionation: comparison of experimental results with theory

“…Examples of continuous electrophoresis methods are continuous two-dimensional electrophoresis, 10 and continuous free-ow electrophoresis. 11 Continuous separation is achieved in the dipole magnetic fractionator (DMF) [12][13][14] using a eld-ow conguration identical to that of continuous free-ow electrophoresis. Turina et al 15 described a continuous twodimensional gas-liquid chromatographic apparatus, but only preliminary experiments were carried out.…”

ELECTRICALLY DRIVEN SEPARATION PROCESSES: ANALYTICAL AND PREPARATIVE METHODS*

“…This radial stress field acts to stabilize the axial flow of carrier fluid against natural convection and thus allows operation of the separator at throughputs in excess of 1 L/h. Commercial versions of this device are available as the Biostream separator (Mattock et al, 1980;Arcus et al, 1980;Thompson, 1983Thompson, , 1984.…”

Two-Dimensional Separation Processes

“…This process has been extensively reviewed (63)(64)(65)(66)(67)(68)(69). A large number of variations of the basic apparatus has been developed.…”

“…Theoretically, flow will be stabilized if the chamber width is reduced to around 5 mm (63). In this case a special collector such as a fan collector (71) needs to be used.…”

“…The rotation causes secondary flows which stabilize the flow. Several different designs of this type have been developed and at least one is commercially available (63,66,68,69,72). Another scheme which does the same type of flow stabilization but without requiring rotating seals is endless belt continuous flow deviation electrophoresis (66,69).…”