Continuous full filling capillary electrochromatography–electrospraying chromatographic nanoparticles

Abstract

Abbreviations CFFContinuous full filling IS AbstractThe influence of instrumental parameters affecting the ionization in continuous full-filling capillary electrochromatography / electrospray ionization mass spectrometry (CFF-CEC/ESI-MS) was investigated. The investigated parameters were the background electrolyte (BGE) and sheath liquid ion strength and organic modifier content, the nebulizer gas pressure, and the concentration of nanoparticles in the BGE. It was found that the nebulizer pressure had the largest influence on the separation efficiency and apparent retention. It was shown that even the lowest pressure investigated was sufficient to guide the nanoparticle flow away from the mass spectrometer inlet. A nebulizer pressure of 5 psi was found being optimal; increasing the pressure significantly decreased the separation efficiency due to the generation of a hydrodynamic flow. Generally, the ion strength of both the BGE and the sheath liquid were found to have very moderate effects on the separation of a homologous series of dialkyl phthalates. Whereas the ionization efficiency was found being unaffected by the nanoparticles, the separation efficiency was found to increase with increasing concentrations up to 3.8 mg/mL, where after it was observed to drop. The optimized method was linear over a wide concentration range and presented limits of detection and quantification more than three-fold lower than what have previously been reported using CFF-CEC/ESI-MS.3

CE and nanomaterials – Part II: Nanomaterials in CE

“…The impact of the presence of NPs in mass spectrometry detection was later investigated in greater detail by Malstrom et al. . Parameters including background electrolyte, sheath liquid ion strength, organic modifier content, nebulizer gas pressure, and concentration of nanoparticles in the electrolyte were examined.…”

Recent advances in micro/nano-particles for clinical detection of cancer biomarkers

“…The NPs can be dynamically adsorbed on the wall of the capillary to change (or reverse) the electroosmotic ow, and they can also act as a pseudostationary phase to participate in the allocation and reservation procedures of the analytes in the column, thereby improving the selectivity and the separation efficiency of the column. 44,45 The recently developed microuidic chip-based technology can complete the whole analytical process of sample preparation, reaction, and detection in biological, chemical, and medical areas. Fan's group developed a CEA microuidic chip based on Au NPs and microuidic technology.…”