Continuous glucose monitoring in interstitial fluid using glucose oxidase-based sensor compared to established blood glucose measurement in rats

Woderer, Stefanie; Henninger, Nadja; Garthe, Claus-Dieter; Kloetzer, Hans Martin; Hajnsek, Martin; Kamecke, Ulrike; Gretz, Norbert; Kraenzlin, Bettina; Pill, Johannes

doi:10.1016/j.aca.2006.08.016

Cited by 32 publications

(22 citation statements)

References 33 publications

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“…This system is based on the principle that ISF glucose levels in the subcutaneous tissue reflect blood glucose levels. The ISF glucose levels range from 87 to 101% of blood glucose levels [14] and, consequently, several needle-type biosensor systems for use in humans have been developed for subcutaneous, intramuscular, and intra-adipose tissue implantation, respectively, to estimate blood glucose levels [8,15]. We have recently developed a biosensor system for continuous blood glucose monitoring in freshwater fish (Nile tilapia, Oreochromis niloticus) which measures glucose in the ISF under the scleral surface of the eyeball (referred to hereafter as EISF) [16,17].…”

Section: Introductionmentioning

confidence: 99%

Wireless monitoring of blood glucose levels in flatfish with a needle biosensor

Endo

Takahashi

Murata³

et al. 2010

Fish Sci

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A wireless biosensor system was developed for the continuous measurement of blood glucose levels in flatfish. The biosensor was implanted in the interstitial fluid under the scleral surface of the eyeball (EISF) to investigate the relationship between EISF and blood glucose levels. EISF glucose levels were found to be correlated with those in the blood and to be approximately the same as blood glucose levels in the range of 7-25 mg dl -1 . A needle-type biosensor was prepared for the continuous EISF glucose monitoring in flatfish. A working electrode was constructed using platinum iridium wire, and glucose oxidase was immobilized to the electrode. The biosensor was inserted into the EISF of flatfish for sensor implantation. A 650-mV potential (vs. Ag/AgCl) was applied by a wireless potentiostat to the working electrode for the amperometric glucose measurement. We investigated whether glucose in the EISF can be determined in vivo.The estimated glucose levels using a one-point calibration method were correlated with actual blood glucose levels. In conclusion, using a wireless biosensor system, we were able to monitor blood glucose levels in flatfish under freeswimming conditions for 16 h.

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Section: Introductionmentioning

confidence: 99%

Wireless monitoring of blood glucose levels in flatfish with a needle biosensor

Endo

Takahashi

Murata³

et al. 2010

Fish Sci

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“…The ideal approach should therefore be noninvasive and continuous glucose monitoring. Several attempts have been made to find a truly suitable means for a continuous glucose monitoring of diabetes [11][12][13]. Some researchers report the use of impedance-based detection system or near-infrared and mid-infrared spectroscopy for noninvasive glucose monitoring [14,15].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Biosensor Based on Bienzyme and Carbon Nanotubes Incorporated into an Os‐complex Thin Film for Continuous Glucose Detection in Human Saliva

et al. 2016

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Saliva opens a door for noninvasive and painless glucose testing since it reflects changes in the body physiology of diabetic individuals as compared to healthy ones. In this paper, a unique, disposable saliva biosensor has been developed for accurate, low cost, and continuous glucose monitoring. The biosensor exhibits linear dependence of the catalytic current upon glucose bulk concentration over the 0.05–1.5 mM range (R=0.998). A detection limit of 0.003 mM can be calculated considering three times the standard deviation of the blank signal divided by the sensitivity of the sensor. The selectivity of the biosensor was evaluated by adding the interferent species of lactate, ascorbic acid and uric acid into in 0.5 mM glucose; the nearly negligible interference current indicates its good selectivity. The operational stability of the biosensor was measured in 1 mM glucose over a 2 h period (RSD=3.27 %). A clinical trial on real‐time noninvasive salivary glucose monitoring was carried out on 30 individuals by measuring subjects’ salivary glucose and blood glucose in parallel. The results show that there is a good correlation of glucose levels in saliva and in blood 2 h after breakfast. Thus, the disposable biosensor would be a potential alternative for continuous glucose detection in human saliva.

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“…The glucose oxidase (GOD)-based biosensor for glucose sensing continues to be the primary model system in the development of new sensing materials and methods [1][2][3][4][5]. Owing to the usefulness in diagnostic analysis of diabetes, GOD-based biosensors have been studied greatly [6][7][8]. In this protocol, the glucose concentration can be obtained indirectly by amperometric detection of the oxidation current of hydrogen peroxide (H 2 O 2 ), a side product during the enzymatic reaction.…”

Section: Introductionmentioning

confidence: 99%