Continuous green biocatalytic processes using ionic liquids and supercritical carbon dioxide

Lozano, Pedro; Diego, Teresa De; Carrié, Daniel; Vaultier, Michel; Iborra, José L.

doi:10.1039/b200055e

Cited by 235 publications

(146 citation statements)

References 13 publications

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“…CALB was suspended in IL on two different supports and was used in 4 h reaction-extraction cycles with scCO 2 as non-reactive phase and stored for 20 h at room temperature (Lozano et al 2002). The synthesis of butyl butyrate from vinyl butyrate and 1-butanol was most successful using [BMIM][NTF 2 ].…”

Section: Introductionmentioning

confidence: 99%

Ionic liquids in biotechnology: applications and perspectives for biotransformations

Roosen

Müller

Greiner

2008

Appl Microbiol Biotechnol

162

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Ionic liquids are considered as an alternative to organic solvents for catalysis. The literature in this field is reviewed with focus on advantageous use of ionic liquids in biocatalysis and biotransformations. The overview reveals that the exploration and mapping of ionic liquids with respect to biocatalysis is still sketchy. It is apparent that advantages can be gained in view of activity, stability and selectivity. Furthermore, integration of reaction and separation has a high potential in the field. The review presents quantitative data on the productivities, space-time yields, as well as stability as far as they can be extracted from the literature.

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Section: Introductionmentioning

confidence: 99%

Ionic liquids in biotechnology: applications and perspectives for biotransformations

Roosen

Müller

Greiner

2008

Appl Microbiol Biotechnol

162

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“…They stated that the recovery of >99.99 mol % was obtained at optimized conditions of 35˝C and 11 MPa. Lozano et al [102] N]/SC-CO 2 systems for the transesterification of vinyl butyratewith 1-butanol and the kinetic resolution of rac-1-phenylethanol with vinyl propionate by CALB. In both systems, the enantiomeric excess of the recovered product fraction (ee p ) was above 99.9% for continuous (R)-1-phenylethyl propionate synthesis at 100˝C and 15 MPa, and the enzyme showed excellent activity and stability.…”

Section: Biocatalysis In Combined Mixture Solvents Of Ionic Liquid Anmentioning

confidence: 99%

Enzyme Stability and Activity in Non-Aqueous Reaction Systems: A Mini Review

et al. 2016

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Abstract:Enormous interest in biocatalysis in non-aqueous phase has recently been triggered due to the merits of good enantioselectivity, reverse thermodynamic equilibrium, and no water-dependent side reactions. It has been demonstrated that enzyme has high activity and stability in non-aqueous media, and the variation of enzyme activity is attributed to its conformational modifications. This review comprehensively addresses the stability and activity of the intact enzymes in various non-aqueous systems, such as organic solvents, ionic liquids, sub-/super-critical fluids and their combined mixtures. It has been revealed that critical factors such as Log P, functional groups and the molecular structures of the solvents define the microenvironment surrounding the enzyme molecule and affect enzyme tertiary and secondary structure, influencing enzyme catalytic properties. Therefore, it is of high importance for biocatalysis in non-aqueous media to elucidate the links between the microenvironment surrounding enzyme surface and its stability and activity. In fact, a better understanding of the correlation between different non-aqueous environments and enzyme structure, stability and activity can contribute to identifying the most suitable reaction medium for a given biotransformation.

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“…Applying enzymes in SC CO 2 /ILs biphasic systems has a very short history. The first successful biotransformation in SC CO 2 /ILs biphasic systems was reported in the year 2002 (Lozano et al, 2002). The protective effect of ILs towards enzyme deactivation by temperature or CO 2 was demonstrated by the observed increase in synthetic activity of the enzyme when it was assayed in the presence of IL.…”

Section: An Overview Of Biocatalysis In Ils and In Scfs/ils Systemsmentioning

confidence: 99%

Application of Ionic Liquids in Biocatalysis

Habulin¹,

Primožič²,

Knez³

2011

Ionic Liquids: Applications and Perspectives

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Continuous green biocatalytic processes using ionic liquids and supercritical carbon dioxide

Abstract: Soluble Candida antarctica lipase B dissolved in ionic liquids showed good synthetic activity, enantioselectivity and operational stability in supercritical carbon dioxide for both butyl butyrate synthesis and the kinetic resolution of 1-phenylethanol processes by transesterification.

Cited by 235 publications

References 13 publications

Ionic liquids in biotechnology: applications and perspectives for biotransformations

Ionic liquids in biotechnology: applications and perspectives for biotransformations

Enzyme Stability and Activity in Non-Aqueous Reaction Systems: A Mini Review

Application of Ionic Liquids in Biocatalysis

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