Continuous harvesting of microalgae by new microfluidic technology for particle separation

Hønsvall, Birgitte K.; Altin, Dag; Robertson, Lucy J.

doi:10.1016/j.biortech.2015.10.046

Cited by 37 publications

(31 citation statements)

References 23 publications

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“…Writ large, these emerging microfluidic technologies have made dramatic advances in a wide range of biomedical (Rafeie et al, 2016;Wu et al, 2012), point of care (Yetisen et al, 2013), drug screening (Skommer & Wlodkowic, 2015), environmental analysis (Jokerst et al, 2012), chemical and biological detection (Sei et al, 2014), and other applications. On the subject of this report, these microfluidic systems have been employed for on-chip detection of microalgal cells (Li et al, 2016), cell culturing (Paik et al, 2017), cell sorting (Juang & Chang, 2016;Schaap et al, 2016), gene sequencing and genome studies (Ghim et al, 2010), cell lysis (Wu et al, 2011), harvesting (Hønsvall et al, 2016;Shakeel Syed et al, 2017) and microbial bioenergy (Han et al, 2013) applications.…”

Section: Introductionmentioning

confidence: 99%

Selective separation of microalgae cells using inertial microfluidics

Syed

Rafeie

Vandamme

et al. 2018

Bioresource Technology

103

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Microalgae represent the most promising new source of biomass for the world's growing demands. However, the biomass productivity and quality is significantly decreased by the presence of bacteria or other invading microalgae species in the cultures. We therefore report a low-cost spiral-microchannel that can effectively separate and purify Tetraselmis suecica (lipid-rich microalgae) cultures from Phaeodactylum tricornutum (invasive diatom). Fluorescent polystyrene-microbeads of 6 μm and 10 μm diameters were first used as surrogate particles to optimize the microchannel design by mimicking the microalgae cell behaviour. Using the optimum flowrate, up to 95% of the P. tricornutum cells were separated from the culture without affecting the cell viability. This study shows, for the first time, the potential of inertial microfluidics to sort microalgae species with minimal size difference. Additionally, this approach can also be applied as a pre-sorting technique for water quality analysis.

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Section: Introductionmentioning

confidence: 99%

Selective separation of microalgae cells using inertial microfluidics

Syed

Rafeie

Vandamme

et al. 2018

Bioresource Technology

103

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“…We show that our deformable nano-sieve device can efficiently separate microalgae from bacteria, enabling controlled movement or trapping of microalgae while mitigating the presence of contaminants. Even though other microfluidic methods have been introduced for microalgae and bacteria separation, they typically require complicated microchannel design and characterizations of the fluid elasticity (Dewan et al, 2012; Hønsvall et al, 2016; Jeonghun et al, 2019). In our case, the separation is achieved by fine-tuning the flow rate and hydrodynamic deformation of the nano-sieve.…”

Section: Discussionmentioning

confidence: 99%

“…However, these traditional cell separation methods have several limitations such as cell damage, labor-intensive processing, high expense, difficulty in scaling, and contamination from reagents used in processing. Microfluidic devices utilizing inertia-based separations have been introduced with the advantages such as low costs, small size, rapid response, and high separation efficiency (Dewan et al,2012; Honsvall et al, 2016). They offer continuous and label-free cell separation, and their ease of use eliminates the need for skilled laboratory technicians to purify microalgal populations through passive filtration.…”

Section: Introductionmentioning

confidence: 99%

SingleChlamydomonas reinhardi(C. reinhardtii) cell separation from bacterial cells and auto-fluorescence tracking with a nano-sieve device

Korensky

Chen

Bao

et al. 2020

Preprint

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16A planar, transparent, and adaptable nano-sieve device is developed for efficient 17 microalgae/bacteria separation. In our strategy, a sacrificial layer is applied in the dual 18 photolithography patterning to achieve a one-dimensional channel with a very low aspect 19 ratio (1:10,000). Microalgae/bacteria mixture is then introduced into the deformable 20 polydimethylsiloxane (PDMS) nano-channel. The hydrodynamic deformation of the nano-21 channel is regulated to allow the bacteria cells to pass through while leaving the microalgae 22 cells trapped in the device. At a flow rate of 4 μl/min, ~100% of the microalgae cells are 23 trapped in the device. Additionally, this device is capable of immobilizing single cells in a 24 transparent channel for auto-fluorescence tracking. These microalgae cells demonstrate 25 minimal photo-bleaching over 250 s laser exposure and can be used to monitor hazardous 26 *Manuscript Click here to view linked References 2 3 4 5 6 7 8 compounds in the sample with a continuous flow fashion. Our method will be valuable to 27 purify microalgae samples containing contaminations and study single cell heterogeneity. 28 29

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“…These natural products represent a great structural diversity, belonging to the polyketide synthase (PKS), non-ribosomal polypeptide synthetase (NRPS), as well as hybrid PKS-NRPS structural classes (20). Microalgae have a broad range of application, like in animal feed and nutritional supplements, in cosmetics, pharmaceuticals, bioremediation and water treatment, renewable energy and others (21,22). They also comprise a wide array of biomolecules such as proteins, lipids, vitamins, pigments, that can be harnessed for commercial use in food, cosmetic and pharmaceutical industry (23).…”

Section: Microalgae As An Inexhaustible Source Of Bioactive Compoundsmentioning

confidence: 99%

Microalgae as a source of high-value bioactive compounds

et al. 2018

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Microalgae are one of the oldest microorganisms, that grow in various hostile environments, ranging from deserts to Antarctica. The microalgae sustain life in such harsh environments through generation of secondary metabolites. Microalgae biosynthesize a large number of diverse bioactive metabolites with activities on cancer, neurodegenerative diseases, and infectious diseases. Here, we highlight the bioactive compounds that are isolated from microalgae for the purpose of using them as food, and as chemicals in pharmaceutical industry as new agents with therapeutic benefits.

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Continuous harvesting of microalgae by new microfluidic technology for particle separation

Cited by 37 publications

References 23 publications

Selective separation of microalgae cells using inertial microfluidics

Selective separation of microalgae cells using inertial microfluidics

SingleChlamydomonas reinhardi(C. reinhardtii) cell separation from bacterial cells and auto-fluorescence tracking with a nano-sieve device

Microalgae as a source of high-value bioactive compounds

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