Continuous High Rate Anaerobic Treatment of Oleic Acid Based Wastewater is Possible after a Step Feeding Start-Up

Cavaleiro, A. J.; Salvador, A. F.; Alves, J. I.; Alves, M. M.

doi:10.1021/es8031264

Cited by 64 publications

(61 citation statements)

References 18 publications

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“…LCFA overloading has been shown to lead to volatile fatty acid (VFA) and LCFA accumulation that may significantly reduce methane production due to imbalances between acid consuming and methane producing microorganisms Angelidaki and Ahring 1992;Eiroa et al 2012;Rinzema et al 1994). Overloading of LCFA can cause concentrations of total volatile acids to be above 2000 mg COD/L or more and/or acetate concentrations above 500 mg COD/ L (Cavaleiro et al 2001;Cavaleiro et al 2009;Eiroa et al 2012). As the total calculated amounts of COD (<55 mg COD/L) and acetate (<15 mg COD/L) produced in this study for microcosms containing biodiesel are much less, they should not be inhibitory.…”

Section: Effects Of the Presence Of Biodiesel In The Anaerobic Degradmentioning

confidence: 99%

Influence of the Anaerobic Biodegradation of Different Types of Biodiesel on the Natural Attenuation of Benzene

Borges¹,

Dias²,

Danko³

2014

Water Air Soil Pollut

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In the present research work, different types of biodiesel were produced by a homogeneous alkali transesterification reaction using soybean oil, pork lard, and castor bean oil as raw materials, to evaluate how their different compositions may affect the biodegradability, namely, in the presence of benzene. Biodiesel was characterized according to the European standard EN 14214. The anaerobic biodegradation of the different types of biodiesel was examined as well as its influence on the biodegradation of benzene. Analyses were performed to determine the volume of methane (directly related to the anaerobic biodegradation of bio-diesel), the concentration of benzene over time, and the production of organic acids. The results showed methane production resulting from the anaerobic degradation of all biodiesel types. The differences between the degradation behavior of each fuel were negligible, contrary to what was expected; however, the amount of methane produced was low due to nutrient limitations. This fact was confirmed by the organic acid analysis as well as by the addition of new media. Anaerobic benzene biodegradation was found to be negatively impacted by the presence of all biodiesel types on average; therefore, the results of this study may impact management of sites that contain biodiesel and fuel hydrocarbon contamination.

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Section: Effects Of the Presence Of Biodiesel In The Anaerobic Degradmentioning

confidence: 99%

Influence of the Anaerobic Biodegradation of Different Types of Biodiesel on the Natural Attenuation of Benzene

Borges¹,

Dias²,

Danko³

2014

Water Air Soil Pollut

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“…The FITC filter reveals SYTO9-stained cells, corresponding to all cells, and the Cy3 filter reveals cells that are stained by both dyes and thus damaged. From each membrane, 10 to 20 randomly selected microscopic fields, each 0.0158 mm 2 , were photographed with a chargecoupled device camera (Olympus DP71) using an image acquisition software (Olympus Cell B). Images obtained with both filters and corresponding to the same microscopic fields were superimposed, and cells with damage and intact membranes were counted.…”

Section: Sourcesmentioning

confidence: 99%

“…A naerobic degradation of long-chain fatty acids (LCFA) is essential for efficient biogas production from complex lipidcontaining wastewaters (1)(2)(3). However, there is still concern regarding the potential toxic effect of LCFA toward methanogenic communities.…”

mentioning

confidence: 99%

Activity and Viability of Methanogens in Anaerobic Digestion of Unsaturated and Saturated Long-Chain Fatty Acids

Sousa

Salvador

Ramos

et al. 2013

Appl Environ Microbiol

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bLipids can be anaerobically digested to methane, but methanogens are often considered to be highly sensitive to the long-chain fatty acids (LCFA) deriving from lipids hydrolysis. In this study, the effect of unsaturated (oleate [C 18:1 ]) and saturated (stearate [C 18:0 ] and palmitate [C 16:0 ]) LCFA toward methanogenic archaea was studied in batch enrichments and in pure cultures. Overall, oleate had a more stringent effect on methanogens than saturated LCFA, and the degree of tolerance to LCFA was different among distinct species of methanogens. Methanobacterium formicicum was able to grow in both oleate-and palmitate-degrading enrichments (OM and PM cultures, respectively), whereas Methanospirillum hungatei only survived in a PM culture. The two acetoclastic methanogens tested, Methanosarcina mazei and Methanosaeta concilii, could be detected in both enrichment cultures, with better survival in PM cultures than in OM cultures. Viability tests using live/dead staining further confirmed that exponential growth-phase cultures of M. hungatei are more sensitive to oleate than are M. formicicum cultures; exposure to 0.5 mM oleate damaged 99% ؎ 1% of the cell membranes of M. hungatei and 53% ؎ 10% of the cell membranes of M. formicicum. In terms of methanogenic activity, M. hungatei was inhibited for 50% by 0.3, 0.4, and 1 mM oleate, stearate, and palmitate, respectively. M. formicicum was more resilient, since 1 mM oleate and >4 mM stearate or palmitate was needed to cause 50% inhibition on methanogenic activity.A naerobic degradation of long-chain fatty acids (LCFA) is essential for efficient biogas production from complex lipidcontaining wastewaters (1-3). However, there is still concern regarding the potential toxic effect of LCFA toward methanogenic communities. Toxicity of LCFA is the main reason for insufficient treatment results of LCFA-containing wastewaters (4-8). Studies of methanogenic inhibition in the rumen confirm the toxicity of these compounds. Soliva et al. (9) showed the antimethanogenic potential of saturated fatty acids of medium chain length, specifically myristate (C 14:0 ) and laurate (C 12:0 ). The adverse effect of fatty acids toward methanogens appears to be more pronounced at longer chain lengths and more unsaturated bonds (10, 11). Moreover, methanogens inhibition by fatty acids is a rapid phenomenon with long recuperation time, as shown by Koster (12): a 50% loss of methanogenic activity was observed after exposing methanogenic sludge to 7.5 mM laurate for only 7.5 min.Sensitivity of microorganisms to LCFA seems to be related to their cell wall structure, with Gram-positive bacteria and methanogens being more easily inhibited than Gram-negative bacteria (13). Inhibition of pure cultures of methanogens by fatty acids is reported in literature (11, 14). Nevertheless, methanogens are ubiquitous in anaerobic bioreactors treating LCFA-rich wastewaters, even over prolonged continuous LCFA loading (15,16). Sousa et al. (16) identified three predominant genera of methanogens in LCFA-degradi...

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“…Period 1 was divided into two cycles: batch phases (10 days) and sequencing continuous feeding phases (30 days). According to Cavaleiro et al [19] and Griffin et al [20] gradual start-up of the mesophilic digester promotes sludge acclimation and improves the digestion process as well. Therefore, the batch assay used during the first 10 days was applied to promote the development of an anaerobic community.…”

Section: Methodsmentioning

confidence: 99%

Enhancement of biogas production from sewage sludge by addition of grease trap sludge

Grosser

Neczaj

2016

Energy Conversion and Management

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Continuous High Rate Anaerobic Treatment of Oleic Acid Based Wastewater is Possible after a Step Feeding Start-Up

Cited by 64 publications

References 18 publications

Influence of the Anaerobic Biodegradation of Different Types of Biodiesel on the Natural Attenuation of Benzene

Influence of the Anaerobic Biodegradation of Different Types of Biodiesel on the Natural Attenuation of Benzene

Activity and Viability of Methanogens in Anaerobic Digestion of Unsaturated and Saturated Long-Chain Fatty Acids

Enhancement of biogas production from sewage sludge by addition of grease trap sludge

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