Continuous Live-Cell Culture Imaging and Single-Cell Tracking by Computational Lensfree LED Microscopy

Abstract

Continuous cell culture monitoring as a way of investigating growth, proliferation, and kinetics of biological experiments is in high demand. However, commercially available solutions are typically expensive and large in size. Digital inline-holographic microscopes (DIHM) can provide a cost-effective alternative to conventional microscopes, bridging the gap towards live-cell culture imaging. In this work, a DIHM is built from inexpensive components and applied to different cell cultures. The images are reconstructed by computational methods and the data are analyzed with particle detection and tracking methods. Counting of cells as well as movement tracking of living cells is demonstrated, showing the feasibility of using a field-portable DIHM for basic cell culture investigation and bringing about the potential to deeply understand cell motility.

Design, Calibration, and Application of a Robust, Cost-Effective, and High-Resolution Lensless Holographic Microscope

“…When comparing our microscope with the existing LHM technologies, it compares favorably in several aspects. For instance, due to its optimized DIHM configuration and optical components, our system experimentally demonstrates higher lateral resolution than most of the reported LHMs [5,14,18,[20][21][22], achieving experimental gain factors of around 2 [5,14,18] or even more [20][21][22]. Only a few contributions reported similar lateral resolutions [12,15].…”

“…In particular, lensless holographic microscopes were reported in the past years, providing a successful miniaturized solution for, just to cite some examples, global healthcare monitoring, point-of-care diagnosis, and biomedical applications [4]. As a common point, lensless microscopes are based on costeffective, light-weight, compact, and portable imaging devices that greatly improve rapid and accurate diagnosis in the field-setting [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22].…”

Recent Advances on Thermal Management of Flexible Inorganic Electronics

“…Microscale inorganic light-emitting diodes, as a typical inorganic electronic device, show excellent interactions with biological tissues as excitation sources. For example, µ-ILEDs can be an alternative excitation source to an optoelectronic tweezer via noncontact manipulation methods to investigate behaviors of various biological samples such as cells [26][27][28][29][30][31][32], proteins [33], DNA molecules [34,35] and particles [36,37]. In addition to the above applications, µ-ILEDs can also be adopted in optogenetics to control, affect and readout the neural activities of biological creatures, especially for stimulation in brain [38][39][40][41], retina [42][43][44], and audition [45][46][47][48][49].…”

Processing and Characterization of Monolithic Passive-Matrix GaN-Based MicroLED Arrays With Pixel Sizes From 5 to 50 µm

“…Besides highly efficient solid-state lighting, ongoing developments of GaN LEDs have driven additional fields of applications, e.g. micro-display [1]- [4] or sensing [5]- [7]. For those purposes, shaping µm-sized emitters with individual control over each pixel is required, most conveniently in a periodic 2D array.…”