Continuous lymphoblastoid suspension cultures from cells of haematopoietic organs of baboons with malignant lymphoma — Biological characterization and biological properties of the herpes virus associated with culture cells

Simian homologues of Epstein–Barr virus

“…Infection of Old World non-human primates with Epstein^Barr virus (EBV) related lymphocryptoviruses (LCVs) was recognized in the early to mid-1970s. Investigators used the newly developed indirect immuno£uorescence assay to detect antibodies cross-reactive to the EBV capsid antigen (VCA) in the serum of many Old World non-human primate species (Dunkel et al 1972;Frank et al 1976;Goldman et al 1968;Kalter et al 1972;Landon & Malan 1971;Levy et al 1971;Naito et al 1971;Stevens et al 1970;Werner et al 1972). The failure to detect EBV crossreactive antibodies in New World primates suggested that LCVs were restricted to humans and non-human Old World primates.…”

“…One of the ¢rst simian LCVs identi¢ed was from baboons at the Institute of Experimental Pathology, USSR Academy of Medical Sciences at Sukhumi. An outbreak of lymphomas in this baboon colony was recognized, beginning in 1967 (Lapin 1974), and lymphoid cell lines containing herpes particles and expressing a crossreactive VCA were established from lymphomatous baboons (Djatchenko et al 1976;Rabin et al 1977). Simian LCV-infected lymphoblastoid cell lines were also established by other investigators using peripheral blood lymphocytes from normal, healthy baboons (Falk et al 1976).…”

Increased antibody responses to Herpes virus papio (HVP) ANTIGENS IN PRE‐LYMPHOMATOUS BABOONS (Papio hamadryas) of the sukhumi high lymphoma stock

“…B-lymphotropic herpes virus -Herpes virus pupio ( H W ) was originally isolated from a lymphomatous baboon of this lymphoma-prone stock (Agrba et al, 1975). Later, it was repeatedly isolated from other lymphomatous and healthy baboons, and it has been shown that HVP was closely related to Epstein-Barr virus (EBV) (Falk et al, 1976(Falk et al, , 1979Djatchenko et al, 1976;Kokosha et al, 1977;Rabin et al, 1977;Deinhardt et al, 1979). Inoculation of HVP-producing cells originating from Sukhumi lymphomatous baboons into marmosets induced fatal acute lymphoproliferative disease (Deinhardt et al, 1978).…”

Antibody responses to herpesvirus papio antigens in baboons with lymphoma

“…Several lymphoblastoid cell lines have been established from tissues of diseased animals (Agbra et al, 1975;Djatchenko et al, 1976;Falk et al, 1976;Rabin et al, 1977). These cell lines have surface properties characteristic of B lymphocytes and contain a herpesvirus (Herpesvirus papio, HVP) that is related to the Epstein-Barr virus (EBV) of man.…”

“…Like EBV, HVP transforms B lymphocytes of human and non-human primate origin, although the two viruses have different but overlapping in vitro host-cell ranges (Rabin et al, 1977). The DNA of HVP shows approximately 40% sequence homology with EBV, and the viruses show considerable antigenic relatedness but can be distinguished on the basis of their nuclear antigens (NA) (Falk et al, 1976;Djatchenko et al, 1976;Rabin et al, 1977) and early antigens (EA) (Neubauer et al, 1978).…”