Continuous monitoring of bacterial biofilm growth using uncoated Thickness-Shear Mode resonators

Castro, Pedro Humberto; Resa, Pablo; Durán, C; Maestre, Juan Ramón; Mateo, M.; Elvira, Luis

doi:10.1088/1757-899x/42/1/012054

Cited by 4 publications

(1 citation statement)

References 6 publications

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“…These two parameters correlate with the density and viscosity of the medium and are very sensitive to the dynamics at the solid-viscous liquid interface and to the appearance of a viscoelastic film that gradually replaces the contact of the QCM with the liquid. The measurement of both parameters could be performed using QCM-D dissipation monitoring techniques [22][23][24][25], by embedding the sensor in the feedback network of an oscillator [26,27] or by extracting them from the electrical impedance analysis [28,29], as in the case of our multi-channel system [30]. Novel system architecture, the measurement procedure, the automatic compensation of the static electrical capacitance C o of the QCM, and the accurate extraction of the series resonance frequency f s and the electrical impedance at resonance R m were introduced.…”

Section: Introductionmentioning

confidence: 99%

Real-Time Detection of the Bacterial Biofilm Formation Stages Using QCM-Based Sensors

et al. 2023

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Bacterial biofilms are a major cause of harm related to medical infections and biofouling. Thus, 80% of total infections are caused by biofilm-forming microorganisms. Consequently, knowledge of biofilm formation stages is crucial to develop effective treatments to prevent their formation in medical implants, tools, and devices. For this purpose, quartz crystal microbalance (QCM) sensors are becoming a good alternative to analytical methods for the real-time monitoring of bacterial growth in liquid media culture. In a previous paper, the authors described an affordable multi-channel measurement instrument based on QCM sensors. However, in order to validate its correct operation, complementary experimental measurements based on bacterial biofilm growth were performed. In this work, the experimental measurements that allow the identification of the different biofilm formation stages are described. The results obtained are discussed.

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