Continuous protein separations in a magnetically stabilized fluidized bed using nonmagnetic supports

Abstract

Continuous protein separations were performed using a magnetically stabilized fluidized bed (MSFB) and a commercially available affinity adsorption resin that contained no magnetically susceptible material. These nonmagnetic materials can be stabilized at relatively low fields (<75 G requiring <30 W) if sufficient magnetically susceptible particles are also present in the stabilized bed. The minimum amount of magnetic particles necessary to stabilize the bed is as low as 20% by volume and is a function of various parameters including the size and density of both particles, the magnetic field strength, and the fluidization velocity. Advantages of these beds for performing separations include true continuous, countercurrent liquid-solids contact, mass-transfer efficiencies nearly equal to that of packed beds, and the ability of handle suspended cells or cell debris. A variety of commercially available affinity, ion-exchange, and adsorptive supports can be used in the bed for continuous separations; results are presented for the adsorption and recovery of lysozyme from an aqueous mixture of lysozyme and myoglobin using an affinity resin.

Magnetic techniques for the isolation and purification of proteins and peptides

“…The minimum amount of magnetic particles necessary to stabilize the bed is a function of various parameters including the size and density of both particles, the magnetic field strength, and the fluidization velocity. A variety of commercially available affinity, ion-exchange, and adsorptive supports can be used in the bed for continuous separations [23]. …”

Pathogenic antibody removal using magnetically stabilized fluidized bed

“…Magnetic adsorbents can be porous or nonporous. They are more commonly manufactured from polymers since they have a variety of surface functional groups which can be tailored to use in different biomedical applications [18][19][20][21][22].…”

Countercurrent gradient chromatography: A continuous focusing technique

“…The multisupport MSFB possesses all of the flow characteristics and mass transfer properties of a single support MSFB and has been used for continuous protein separations. 6 In this article, we will describe the basic phenomena of CGC and discuss experimental and theoretical results that we have obtained. We use a multisupport MSFB system to perform the experimental separation and focusing of proteins.…”