Continuous suspension fractionation using acoustic and divided-flow fields

Mandralis, Zenon I.; Feke, Donald L.

doi:10.1016/0009-2509(93)80368-z

Cited by 33 publications

(16 citation statements)

References 12 publications

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“…Instead, we measure the voltage applied to the driving transducer and use the multilayer resonator model of Rusinko (2000) to compute the acoustic energy density within the chamber. This model has proved suc cessful in other applications (Mandralis and Feke, 1993;Pangu and Feke, 2004).…”

Section: Model For the Separation Processmentioning

confidence: 99%

“…Previous work has shown that particles with positive acoustic contrast factor move towards the pressure nodes and, conversely, particles with negative F move towards the pressure antinodes in the absence of any other forces acting on them (Hawkes et al, 1998a, b;Johnson and Feke, 1995;Mandralis and Feke, 1993).…”

Section: Introductionmentioning

confidence: 98%

“…F depends on the densities of the suspending fluid and the solid, and the relative compressibility of the fluid and solid, given by (Mandralis and Feke, 1993):…”

Section: Introductionmentioning

confidence: 99%

“…Based on these concepts, Mandralis and Feke (1993) demonstrated the size-fractionation of a population of 2 -30 Am polystyrene particles in aqueous suspension. Since no inlet splitter was used in this work, fractionation effi ciency was relatively low.…”

Section: Introductionmentioning

confidence: 99%

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Fractionation of cell mixtures using acoustic and laminar flow fields

Kumar

Feke

Belovich

2004

Biotechnol. Bioeng.

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A fractionation method applicable to different populations of cells in a suspension is reported. The sepa ration was accomplished by subjecting the suspension to a resonant ultrasonic field and a laminar flow field propa gating in orthogonal directions within a thin, rectangular chamber. Steady, laminar flow transports the cell suspen sion along the chamber, while the ultrasonic field causes the suspended cells to migrate to the mid-plane of the chamber at rates related to their size and physical proper ties. A thin flow splitter positioned near the outlet divides the effluent cell suspension into two product streams, thereby allowing cells that respond faster to the acoustic field to be separated from those cells that respond more slowly. Modeling of the trajectories of individual cells through the chamber shows that by altering the strength of the flow relative to that of the acoustic field, the desired fractionation can be controlled. Proof-of-concept experi ments were performed using hybridoma cells and Lactoba cillus rhamnosus cells. The two populations of cells could be effectively separated using this technique, resulting in hybridoma/Lactobacillus ratios in the left and right prod uct streams, normalized to the feed ratio, of 6.9 F 1.8 and 0.39 F 0.01 (vol/vol), respectively. The acoustic method is fast, efficient, and could be operated continuously with a high degree of selectivity and yield and with low power consumption.

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Section: Model For the Separation Processmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

“…F depends on the densities of the suspending fluid and the solid, and the relative compressibility of the fluid and solid, given by (Mandralis and Feke, 1993):…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fractionation of cell mixtures using acoustic and laminar flow fields

Kumar

Feke

Belovich

2004

Biotechnol. Bioeng.

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“…In the early 1990s, Feke and collaborators investigated the combination of acoustic standing wave particle banding and laminar flow-based separators in order to collect particles based on their acoustic properties and size (Johnson and Feke 1995;Mandralis and Feke 1993). Mandralis and Feke (1993) demonstrated that the acoustic contrast factor and size of a particle determines its response to acoustic radiation force.…”

Section: Introductionmentioning

confidence: 99%

Viscous effects in the acoustic manipulation of algae for biofuel production

Leckey

Hinders

2011

J Appl Phycol

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Microalgae are emerging as a promising source for environmentally friendly biofuels. Acoustic manipulation of algal cells using standing waves is a relatively new method for dewatering and/or sorting algae harvests. Recent work in the field has shown that acoustic dewatering methods may be more efficient and economical than traditional methods. Optimization of acoustic algal cell manipulation requires a knowledge of the acoustic radiation force upon the cells. Previous work in the field does not account for viscosity of the algal cells or surrounding fluid. We have implemented inviscid and viscous acoustic force models for standing waves incident upon algal cells in salt and freshwater. The results presented in this paper show that significant viscous effects can occur at certain frequencies and/or cell sizes and may need to be taken into account in the development of efficient experimental techniques.

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Fractionation of suspensions using synchronized ultrasonic and flow fields

Mandralis

Feke

1993

AIChE Journal

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A fractionation method for fine-particle suspensions using resonant ultrasonic fields coordinated with bidirectional fluid flow fields is described. The basis for the separation is differences in the speed of response of particles to the imposition of a resonant acoustic field. As such, the method is sensitive to the particle size and the acoustic contrast between the solid particles and their suspending fluid. Both batch and continuous fractionation processes can be developed from a two-step acoustic-flow cycle. An analytical model was constructed from equations that describe the trajectories of particles as they respond to the acoustic and flow fields. Model predictions indicate how the fractionation can be controlled through choice of cycle parameters. The method was implemented experimentally. Results for the fractionation of 325-mesh polystyrene spheres indicate that sharp fractionations can be achieved. The experimental results are generally in agreement with the model predictions.

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Continuous suspension fractionation using acoustic and divided-flow fields

Cited by 33 publications

References 12 publications

Fractionation of cell mixtures using acoustic and laminar flow fields

Fractionation of cell mixtures using acoustic and laminar flow fields

Viscous effects in the acoustic manipulation of algae for biofuel production

Fractionation of suspensions using synchronized ultrasonic and flow fields

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