Contrast-Enhanced Gray-Scale Ultrasound for Quantitative Evaluation of Tumor Response to Chemotherapy: Preliminary Results With a Mouse Hepatoma Model

Zhou, Jian; Cao, Long; Zheng, Wei; Liu, Min; Han, Feng; Li, An Hua

doi:10.2214/ajr.10.4734

Cited by 20 publications

(13 citation statements)

References 26 publications

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“…The presence of UCA has proven to be a good overall indicator of blood volume within tumors. 6,[30][31][32] Recently, Needles et al described the development of the VisualSonics PA system and used it to monitor the response of a breast cancer cell line in mice to a targeted anti-angiogenic therapy. 26 The group showed a significant decrease in CEUS-derived tumor vascularity and tumor perfusion as well as PA-derived blood and tissue oxygenation levels between the treatment and control groups.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Photoacoustically Derived Hemoglobin and Oxygenation Measurements with Contrast-Enhanced Ultrasound Estimated Vascularity and Immunohistochemical Staining in a Breast Cancer Model

et al. 2014

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In this preliminary study, we compared two noninvasive techniques for imaging intratumoral physiological conditions to immunohistochemical staining in a murine breast cancer model. MDA-MB-231 tumors were implanted in the mammary pad of 11 nude rats. Ultrasound and photoacoustic (PA) scanning were performed using a Vevo 2100 scanner (Visualsonics, Toronto, Canada). Contrast-enhanced ultrasound (CEUS) was used to create maximum intensity projections as a measure of tumor vascularity. PAs were used to determine total hemoglobin signal (HbT), oxygenation levels in detected blood (SO2 Avg), and oxygenation levels over the entire tumor area (SO2 Tot). Tumors were then stained for vascular endothelial growth factor (VEGF), cyclooxygenase-2 (Cox-2), and the platelet endothelial cell adhesion molecule CD31. Correlations between findings were analyzed using Pearson's coefficient. Significant correlation was observed between CEUS-derived vascularity measurements and both PA indicators of blood volume (r = 0.49 for HbT, r = 0.50 for SO2 Tot). Cox-2 showed significant negative correlation with SO2 Avg (r = -0.49, p = 0.020) and SO2 Tot (r = -0.43, p = 0.047), while CD31 showed significant negative correlation with CEUS-derived vascularity (r = -0.47, p = 0.036). However, no significant correlation was observed between VEGF expression and any imaging modality (p > 0.08). Photoacoustically derived HbT and SO2 Tot may be a good indicator of tumor fractional vascularity. While CEUS correlates with CD31 expression, photoacoustically derived SO2 Avg appears to be a better predictor of Cox-2 expression.

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Section: Discussionmentioning

confidence: 99%

Comparison of Photoacoustically Derived Hemoglobin and Oxygenation Measurements with Contrast-Enhanced Ultrasound Estimated Vascularity and Immunohistochemical Staining in a Breast Cancer Model

et al. 2014

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“…The bolus injection was performed by one radiologist (W.Z., with 3 years of experience in small-animal studies) within 1-2 seconds for all animals, University) of 5 to 6 weeks old, weighing from 16 to 18 g ( 27 ). On the basis of our previous experience with this tumor model ( 28 ), the sample size of 10 mice per treatment group was selected to provide a signifi cant difference in tumor volume of 50% between the control group and the treated groups, with a power fi xed at 0.8 and a type I error of a = .05.…”

Section: Histologic Analysismentioning

confidence: 99%

Quantitative Assessment of Tumor Blood Flow in Mice after Treatment with Different Doses of an Antiangiogenic Agent with Contrast-enhanced Destruction-Replenishment US

Zhou¹,

Cao²,

Liu³

et al. 2011

Radiology

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“…Other than these molecular target drugs, currently most of the cancer patents still undergo the treatment of cytotoxic chemotherapy. We have previously shown that tumor blood flow perfusion changes after cisplatin treatment with CEUS [12]. However, to the best of our knowledge, the value of quantifying tumor blood flow perfusion with CEUS has not been tested in early assessing tumor response to cytotoxic chemotherapy.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Early Tumor Response to Cytotoxic Chemotherapy with Dynamic Contrast-Enhanced Ultrasound in Human Breast Cancer Xenografts

et al. 2013

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There is a strong need to assess early tumor response to chemotherapy in order to avoid adverse effects from unnecessary chemotherapy and allow early transition to second-line therapy. This study was to quantify tumor perfusion changes with dynamic contrast-enhanced ultrasound (CEUS) in the evaluation of early tumor response to cytotoxic chemotherapy. Sixty nude mice bearing with MCF-7 breast cancer were administrated with either adriamycin or sterile saline. CEUS was performed on days 0, 2, 4 and 6 of the treatment, in which time-signal intensity (SI) curves were obtained from the intratumoral and depth-matched liver parenchyma. Four perfusion parameters including peak enhancement (PE), area under the curve of wash-in (WiAUC), wash-in rate (WiR) and wash-in perfusion index (WiPI) were calculated from perfusion curves and normalized with respect to perfusion of adjacent liver parenchyma. Histopathological analysis was conducted to evaluate tumor perfusion, tumor cell density, microvascular density (MVD) and proliferating cell density. Significant decreases of tumor normalized perfusion parameters (i.e., nPE, nWiAUC, nWiR and nWiPI) were noticed between adriamycin-treated and control groups (P<0.01) 2 days after therapy. There were significant differences of tumor volumes between control and treated groups on day 6 (P<0.001) while there were no significant differences in tumor volume on days 0, 2 and 4 (P>0.05). Significant decreases of tumor perfusion, tumor cell density, MVD and proliferating cell density were seen in adrianycin-treated group 2 days after therapy when compared to control group (P<0.001). Dynamic CEUS for quantification of tumor perfusion could be used for early detection of cancer response to cytotoxic chemotherapy prior to notable tumor shrinkage.

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Contrast-Enhanced Gray-Scale Ultrasound for Quantitative Evaluation of Tumor Response to Chemotherapy: Preliminary Results With a Mouse Hepatoma Model

Abstract: Quantitative analysis of tumor blood perfusion with contrast-enhanced ultrasound can be used for noninvasive assessment of functional changes in tumors after chemotherapy.

Cited by 20 publications

References 26 publications

Comparison of Photoacoustically Derived Hemoglobin and Oxygenation Measurements with Contrast-Enhanced Ultrasound Estimated Vascularity and Immunohistochemical Staining in a Breast Cancer Model

Comparison of Photoacoustically Derived Hemoglobin and Oxygenation Measurements with Contrast-Enhanced Ultrasound Estimated Vascularity and Immunohistochemical Staining in a Breast Cancer Model

Quantitative Assessment of Tumor Blood Flow in Mice after Treatment with Different Doses of an Antiangiogenic Agent with Contrast-enhanced Destruction-Replenishment US

Assessment of Early Tumor Response to Cytotoxic Chemotherapy with Dynamic Contrast-Enhanced Ultrasound in Human Breast Cancer Xenografts

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