Contrast Enhancement of Optical Coherence Tomography Images Using Branched Gold Nanoparticles

León, Yenisey Ponce de; Pichardo‐Molina, J. L.; Ochoa, Noé Alcalá; Luna-Moreno, Donato

doi:10.1155/2012/571015

Cited by 18 publications

(17 citation statements)

References 42 publications

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“…The slope of the in-depth OCT signals correlates with the scattering coefficient of the medium. The backward scattering of the silica-coated nanostars, especially those with the thickest shell, is higher than the intensity of the signal from bare nanostars, which is in good agreement with previous reports 26 and measured spectral data (see Fig. However, the multiple scattering significantly complicates this relation.…”

Section: Optical Coherence Tomography Experimentssupporting

confidence: 92%

“…Despite their importance as contrast agents in biophotonics, scattering characteristics attract much less attention from researchers. 26 Due to the high scattering, NPs are attractive agents for visualizing living cells and the dynamic processes occurring in them by using optical imaging techniques such as confocal, 27 differential interference contrast, 28 and dark-field 29 microscopic techniques. The latter is a noninvasive technique based on low-coherence interferometry utilizing backscattered light for in vivo imaging of layered tissues, allowing for distinguishing between normal and pathological tissue and for monitoring changes in optical properties.…”

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confidence: 99%

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Optical properties of plasmon-resonant bare and silica-coated nanostars used for cell imaging

et al. 2015

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We synthesized and characterized gold nanostars and their silica-coated derivatives with 7- to 50-nm shell thicknesses as contrast agents for optical imaging. The scattering and absorption coefficients of the nanoparticles (NPs) were estimated by means of collimated transmittance and diffuse reflectance/transmittance analyses. The contrasting properties of the nanostructures were studied in optical coherence tomography glass capillary imaging. The silica-coated nanostars with the thickest shell have higher scattering ability in comparison with bare nanostars. Viability assays confirmed weak in vitro toxicity of nanostructures at up to ∼200-μg/mL concentrations. We showed real-time visualization of nanostars in both agarose and cultured cells by analyzing the backscattering signal using a conventional laser confocal microscope. The signal intensity detected from the silica-coated NPs was almost 1.5 times higher in comparison with bare nanostars. To the best of our knowledge, this is the first time that conventional laser confocal microscopy was applied in combined scattering and transmitted light modes to detect the backscattered signal of gold nanostars, which is useful for direct monitoring of the uptake, translocation, and accumulation of NPs in living cells.

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Section: Optical Coherence Tomography Experimentssupporting

confidence: 92%

mentioning

confidence: 99%

Optical properties of plasmon-resonant bare and silica-coated nanostars used for cell imaging

et al. 2015

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“…GNP can be used as contrast agents in optical coherence tomography (OCT) for diagnosis . OCT is a widely used, nondestructive and noninvasive 3D interferometry microscopy technique.…”

Section: Gold Nanoparticles For Diagnosis In Ophthalmologymentioning

confidence: 99%

Gold nanoparticles in ophthalmology

Masse

Ouellette

Lamoureux

et al. 2018

Medicinal Research Reviews

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Many research projects are underway to improve the diagnosis and therapy in ophthalmology. Indeed, visual acuity deficits affect 285 million people worldwide and different strategies are being developed to strengthen patient care. One of these strategies is the use of gold nanoparticles (GNP) for their multiple properties and their ability to be used as both diagnosis and therapy tools. This review exhaustively details research developing GNPs for use in ophthalmology. The toxicity of GNPs and their distribution in the eye are described through in vitro and in vivo studies. All publications addressing the pharmacokinetics of GNPs administered in the eye are extensively reviewed. In addition, their use as biosensors or for imaging with optical coherence tomography is illustrated. The future of GNPs for ophthalmic therapy is also discussed. GNPs can be used to deliver genes or drugs through different administration routes. Their antiangiogenic and anti-inflammatory properties are of great interest for different ocular pathologies. Finally, GNPs can be used to improve stereotactic radiosurgery, brachytherapy, and photothermal therapy because of their many properties.

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“…AuNPs show excellent stability, universal biocompatibility, and unique optical, surface, electronic, and photocatalytic properties [1][2][3][4]. The biocompatibility and optical property have contributed to the increasing interest in utilizing AuNPs as optical markers for living cells [5], colorimetric sensors [6], contrast agent for optical coherence tomography imaging [7], and vehicles for drug and gene delivery [8]. The properties of AuNPs attribute to their surface plasmon resonance (SPR) property which is dependet upon their particle characteristics (shape and size) [9].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Gold Nanochains with Octreotide Acetate Template

Zhou

Gao

et al. 2013

Journal of Nanomaterials

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We described a facile method for assembly gold nanochains by using octreotide acetate as template in aqueous environment. In acidic solution, octreotide acetate was conferred positive charges and its structure changed to chain-like. The monodisperse negative gold nanoparticles were bound to the surface of octreotide acetate template by electrostatic attraction and the interaction of gold nanoparticles with amino acid residues (tryptophan and lysine). The fabricated gold nanostructure presented chain-like observed by transmission electron microscopy. The cytotoxicity of gold nanochains was examined by tetrazolium dye-based microtitration (MTT) assay, which demonstrated significantly less toxicity than that of octreotide acetate alone. The MTT assay also reflected the combinative action between the gold nanoparticles with octreotide acetate. Our work lays the groundwork for developing octreotide acetate-templated nanomaterials that can be used as a building block for the creation of nanomaterials. Meanwhile, the harmfulless gold nanochains have great application prospects in the biomedical filed.

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Contrast Enhancement of Optical Coherence Tomography Images Using Branched Gold Nanoparticles

Cited by 18 publications

References 42 publications

Optical properties of plasmon-resonant bare and silica-coated nanostars used for cell imaging

Optical properties of plasmon-resonant bare and silica-coated nanostars used for cell imaging

Gold nanoparticles in ophthalmology

Fabrication of Gold Nanochains with Octreotide Acetate Template

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