2019
DOI: 10.3390/microorganisms7080230
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Contrast of Real-Time Fluorescent PCR Methods for Detection of Escherichia coli O157:H7 and of Introducing an Internal Amplification Control

Abstract: Various constituents in food specimens can inhibit the PCR assay and lead to false-negative results. An internal amplification control was employed to monitor the presence of false-negative results in PCR amplification. In this study, the objectives were to compare the real-time PCR-based method by introducing a competitive internal amplification control (IAC) for the detection of Escherichia O157:H7 with respect to the specificity of the primers and probes, analytical sensitivity, and detection limits of cont… Show more

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Cited by 9 publications
(6 citation statements)
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“…Over the years, bacteria have developed complex mechanisms to cope with environmental stress and stress in order to survive in the harshest environments, including the human body. To gain an advantage, bacteria need to compete for nutrients and avoid attacks from molecules produced by other competing organisms [26]. In a particular host, bacteria are constantly attacked by the host's immune system, and in order to establish themselves in a particular biological environment, it is essential that they adapt and cope with these stressful situations.…”
Section: Microbial Resistance Mechanismsmentioning
confidence: 99%
“…Over the years, bacteria have developed complex mechanisms to cope with environmental stress and stress in order to survive in the harshest environments, including the human body. To gain an advantage, bacteria need to compete for nutrients and avoid attacks from molecules produced by other competing organisms [26]. In a particular host, bacteria are constantly attacked by the host's immune system, and in order to establish themselves in a particular biological environment, it is essential that they adapt and cope with these stressful situations.…”
Section: Microbial Resistance Mechanismsmentioning
confidence: 99%
“…It poses a severe threat to human health, potentially leading to mild diarrhoea, haemorrhagic enteritis, and haemolytic‐uremic syndrome. Since the minimum dose of E. coli O157:H7 infection is very low, [32] the need for a highly sensitive, convenient, rapid and reliable detection method is even greater.…”
Section: Application Of Aptamer Electrochemical Biosensors For the De...mentioning
confidence: 99%
“…coli é uma das três principais bactérias patogênicas transmitidas por alimentos e pela água no mundo, sendo clinicamente importante, pois sua infecção pode levar a uma ampla gama de manifestações clínicas, incluindo infecções assintomáticas, diarreia leve ou doenças graves, como colite hemorrágica e síndrome hemolítico-urêmica. Um surto causado por este patógeno pode atingir proporções epidêmicas, causando um ônus econômico considerável 39 .…”
Section: E Coli Tem Sido Utilizada Como O Principal Indicador De Coli-unclassified