Contrasting effects of parathyroid hormone and 1,25-dihydroxyvitamin D3 on human osteoblasts in culture

2D-PAGE reveals the absence of collagenase production in normal human osteoblasts in culture

“…The osteoblasts were grown for one month in culture using an established method [2] and had been passaged three times. Osteoblasts cultured for several assa es (over about 5 months) are reported to undergo a Lss of well defined osteoblastic phenotype [3].…”