Contribution of Apical and Basal Dendrites of L2/3 Pyramidal Neurons to Orientation Encoding in Mouse V1

Park, Jiyoung; Papoutsi, Athanasia; Ash, Ryan T.; Marin, Miguel A.; Poirazi, Panayiota; Smirnakis, Stelios M.

doi:10.1101/566588

Cited by 13 publications

(18 citation statements)

References 53 publications

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“…This, combined with the fact that CaMKII expression is higher in superficial L2/3 neurons than L4 or L5 neurons (Lein, 2007;Tighilet et al, 1998), could result in an increased effect of the CaMKII inhibition in superficial synapses. Long-range cortical input, which is thought to carry motor-related input to V1 (Leinweber et al, 2017), arrives preferentially on more superficial synapses than the bottom-up visual input (Park et al, 2019;Petreanu et al, 2009;Young et al, 2021). Thus, the differences in effect on grating responses between the NMDA receptor knockout and the CaMKII inhibition could be explained by a differential influence on top-down and bottom-up pathways.…”

Section: Discussionmentioning

confidence: 99%

NMDA receptors in visual cortex are necessary for normal visuomotor integration and skill learning

Widmer

O’Toole

Keller

2022

eLife

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The experience of coupling between motor output and visual feedback is necessary for the development of visuomotor skills and shapes visuomotor integration in visual cortex. Whether these experience dependent changes of responses in V1 depend on modifications of the local circuit or are the consequence of circuit changes outside of V1 remains unclear. Here, we probed the role of NMDA receptor dependent signaling, which is known to be involved in neuronal plasticity, in mouse primary visual cortex (V1) during visuomotor development. We used a local knockout of NMDA receptors and a photoactivatable inhibition of CaMKII in V1 during first visual experience to probe for changes in neuronal activity in V1 as well as the influence on performance in a visuomotor task. We found that a knockout of NMDA receptors before, but not after, first visuomotor experience reduced responses to unpredictable stimuli, diminished the suppression of predictable feedback in V1, and impaired visuomotor skill learning later in life. Our results demonstrate that NMDA receptor dependent signaling in V1 is critical during first visuomotor experience for shaping visuomotor integration and enabling visuomotor skill learning.

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Section: Discussionmentioning

confidence: 99%

NMDA receptors in visual cortex are necessary for normal visuomotor integration and skill learning

Widmer

O’Toole

Keller

2022

eLife

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“…However, existing and emerging technologies that combine multiphoton microscopy with optogenetics now allow for manipulation of activity in experimenter-defined neurons in 3-dimensional space (Yang et al, 2018;Marshel et al, 2019). Furthermore, multiphoton laser-directed lesion experiments have been employed at the level of cell bodies, axons, dendrites, and dendritic spines in vivo (Allegra Mascaro et al, 2010;Canty et al, 2013;Hill et al, 2017;Park et al, 2019). These technologies could now be combined with drug self-administration studies to identify the function of unique neuronal ensembles and morphological plasticity for drug use and seeking.…”

Section: Evaluating the Function Of Neuronal Ensemble Activity Patterns And Morphological Plasticity In Drug Use And Seekingmentioning

confidence: 99%

A Novel Assay Allowing Drug Self-Administration, Extinction, and Reinstatement Testing in Head-Restrained Mice

Vollmer

Doncheck

Grant

et al. 2021

Front. Behav. Neurosci.

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Multiphoton microscopy is one of several new technologies providing unprecedented insight into the activity dynamics and function of neural circuits. Unfortunately, some of these technologies require experimentation in head-restrained animals, limiting the behavioral repertoire that can be integrated and studied. This issue is especially evident in drug addiction research, as no laboratories have coupled multiphoton microscopy with simultaneous intravenous drug self-administration, a behavioral paradigm that has predictive validity for treatment outcomes and abuse liability. Here, we describe a new experimental assay wherein head-restrained mice will press an active lever, but not inactive lever, for intravenous delivery of heroin or cocaine. Similar to freely moving animals, we find that lever pressing is suppressed through daily extinction training and subsequently reinstated through the presentation of relapse-provoking triggers (drug-associative cues, the drug itself, and stressors). Finally, we show that head-restrained mice will show similar patterns of behavior for oral delivery of a sucrose reward, a common control used for drug self-administration experiments. Overall, these data demonstrate the feasibility of combining drug self-administration experiments with technologies that require head-restraint, such as multiphoton imaging. The assay described could be replicated by interested labs with readily available materials to aid in identifying the neural underpinnings of substance use disorder.

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“…Recently, focal laser ablations have been employed in investigating the contributions of single cells or single dendrites to sensory representation or orientation tuning, respectively [ 36 , 37 ]. In these studies, focal laser ablations were not performed deeper than cortical layer II/III because of the limitation of the maximum depth [ 13 , 14 ], which prevented detailed investigations of the functions of excitatory cortico–cortical connections across various cortical layers [ 38 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

In vivo two-photon microscopic observation and ablation in deeper brain regions realized by modifications of excitation beam diameter and immersion liquid

et al. 2020

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In vivo two-photon microscopy utilizing a nonlinear optical process enables, in living mouse brains, not only the visualization of morphologies and functions of neural networks in deep regions but also their optical manipulation at targeted sites with high spatial precision. Because the two-photon excitation efficiency is proportional to the square of the photon density of the excitation laser light at the focal position, optical aberrations induced by specimens mainly limit the maximum depth of observations or that of manipulations in the microscopy. To increase the two-photon excitation efficiency, we developed a method for evaluating the focal volume in living mouse brains. With this method, we modified the beam diameter of the excitation laser light and the value of the refractive index in the immersion liquid to maximize the excitation photon density at the focal position. These two modifications allowed the successful visualization of the finer structures of hippocampal CA1 neurons, as well as the intracellular calcium dynamics in cortical layer V astrocytes, even with our conventional two-photon microscopy system. Furthermore, it enabled focal laser ablation dissection of both single apical and single basal dendrites of cortical layer V pyramidal neurons. These simple modifications would enable us to investigate the contributions of single cells or single dendrites to the functions of local cortical networks.

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Contribution of Apical and Basal Dendrites of L2/3 Pyramidal Neurons to Orientation Encoding in Mouse V1

Cited by 13 publications

References 53 publications

NMDA receptors in visual cortex are necessary for normal visuomotor integration and skill learning

NMDA receptors in visual cortex are necessary for normal visuomotor integration and skill learning

A Novel Assay Allowing Drug Self-Administration, Extinction, and Reinstatement Testing in Head-Restrained Mice

In vivo two-photon microscopic observation and ablation in deeper brain regions realized by modifications of excitation beam diameter and immersion liquid

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