Contribution of CXCR3-mediated signaling in the metastatic cascade of solid malignancies

Cannon, Andrew; Thompson, Christopher; Bhatia, Rakesh; Kandy, Rakhee Rathnam Kalari; Solheim, Joyce C.; Batra, Surinder K.; Kumar, Sushil

doi:10.1016/j.bbcan.2021.188628

Cited by 5 publications

(1 citation statement)

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“…Among the transcripts coding for peptidic agonists, we identified GPCR ligands, such as the CXCL9 chemokine, and growth factors known as agonists of tyrosine kinase receptors ( HBEGF, ANGPTL6 , Figure 2C ), cytokines ( IL7, IL15 , Figure 2C ), and agonists of serine/threonine kinase receptors ( BMP1 , TGFB3 , Figure 2C ). Of the GPCRs and their agonists that showed clear contrast in their Spearman correlation values comparing the high and low PREX1 expression groups, we found a known chemokine-GPCR pair, CXCL9-CXCR3 , already described as relevant in metastatic cancer progression ( 54 ). The high PREX1 expression group included 4 growth factor receptors that showed higher Spearman’s coefficient in the high versus the low PREX1 expression groups; this set included ephrin ( EPHA1 ), FGFR1 , and VEGF receptors ( NRP1/2 ) ( Figure 2C ).…”

Section: Resultsmentioning

confidence: 85%

P-Rex1 Signaling Hub in Lower Grade Glioma Patients, Found by In Silico Data Mining, Correlates With Reduced Survival and Augmented Immune Tumor Microenvironment

2022

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Systematic analysis of tumor transcriptomes, combined with deep genome sequencing and detailed clinical assessment of hundreds of patients, constitutes a powerful strategy aimed to identify potential biomarkers and therapeutic targets to guide personalized treatments. Oncogenic signaling cascades are integrated by multidomain effector proteins such as P-Rex1, a guanine nucleotide exchange factor for the Rac GTPase (RacGEF), known to promote metastatic dissemination of cancer cells. We hypothesized that patients with high P-Rex1 expression and reduced survival might be characterized by a particular set of signaling proteins co-expressed with this effector of cell migration as a central component of a putative signaling hub indicative of poor prognosis. High P-Rex1 expression correlated with reduced survival of TCGA Lower Grade Glioma (LGG) patients. Thus, guided by PREX1 expression, we searched for signaling partners of this RacGEF by applying a systematic unbiased in silico data mining strategy. We identified 30 putative signaling partners that also correlated with reduced patient survival. These included GPCRs such as CXCR3, GPR82, FZD6, as well as MAP3K1, MAP2K3, NEK8, DYRK3 and RPS6KA3 kinases, and PTPN2 and PTPN22 phosphatases, among other transcripts of signaling proteins and phospho-substrates. This PREX1 signaling hub signature correlated with increased risk of shorter survival of LGG patients from independent datasets and coincided with immune and endothelial transcriptomic signatures, indicating that myeloid infiltration and tumor angiogenesis might contribute to worsen brain tumor pathology. In conclusion, P-Rex1 and its putative signaling partners in LGG are indicative of a signaling landscape of the tumor microenvironment that correlates with poor prognosis and might guide the characterization of signaling targets leading the eventual development of immunotherapeutic strategies.

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Section: Resultsmentioning

confidence: 85%