Contribution of KCTD12 to esophageal squamous cell carcinoma

Abbaszadegan, Mohammad Reza; Taghehchian, Negin; Li, Liping; Aarabi, Azadeh; Moghbeli, Meysam

doi:10.1186/s12885-018-4765-z

Cited by 19 publications

(17 citation statements)

References 34 publications

(41 reference statements)

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“…This protein is one of the potassium channel tetramerization domain (KCTD) protein families that has a role as an auxiliary subunit of GABAB receptors to alter the emotionality and neuronal excitability [ 33 ]. The expression of KCTD12 transcript and protein was associated with multiple tumor types including gastrointestinal stromal, melanoma, and esophageal squamous cell [ 34 , 35 , 36 ]. Here, we report significant upregulation of KCTD12 in the KAIMRC1 cells in spite of the fact that previous proteomics-based results suggest downregulation of this protein in a breast cancer tissue isolated from a patient with invasive carcinoma [ 37 ].…”

Section: Resultsmentioning

confidence: 99%

Proteomics Profiling of KAIMRC1 in Comparison to MDA-MB231 and MCF-7

Alghanem

Ali

Nehdi

et al. 2020

IJMS

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Proteomics characterization of KAIMRC1 cell line, a naturally immortalized breast cancer cells, is described in comparison to MCF-7 and MDA-MB-231 breast cancer cells. Quantitative proteomics analysis using the tandem mass tag (TMT)-labeled technique in conjunction with the phosphopeptide enrichment method was used to perform comparative profiling of proteins and phosphoproteins in the three cell lines. In total, 673 proteins and 33 Phosphoproteins were differentially expressed among these cell lines. These proteins are involved in several key cellular pathways that include DNA replication and repair, splicing machinery, amino acid metabolism, cellular energy, and estrogen signaling pathway. Many of the differentially expressed proteins are associated with different types of tumors including breast cancer. For validation, 4 highly significant expressed proteins including S-methyl-5′-thioadenosine phosphorylase (MTAP), BTB/POZ domain-containing protein (KCTD12), Poly (ADP-ribose) polymerase 1 (PARP 1), and Prelamin-A/C were subjected to western blotting, and the results were consistent with proteomics analysis. Unlike MCF-7 and MDA-MB-231, KAIMRC1 showed different phospho- and non-phosphoproteomic phenotypes which make it a potential model to study breast cancer.

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Section: Resultsmentioning

confidence: 99%

Proteomics Profiling of KAIMRC1 in Comparison to MDA-MB231 and MCF-7

Alghanem

Ali

Nehdi

et al. 2020

IJMS

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“…11 Abbaszadegan et al showed that KCTD12 can suppress Wnt/Notch signaling, stem cell factors, and chromatin remodelers in esophageal squamous cell carcinoma. 12 Nonetheless, KCTD12 is dramatically upregulated and closely correlated with larger tumor sizes, higher pathologic stages, and poor survival in cervical and lung cancers. 13 especially, knockdown of FOXO1 can restore the inhibitory effect of KCTD12 on cell proliferation of breast cancer.…”

Section: Potassium Channel Tetramerization Domain Containing 12mentioning

confidence: 99%

“…Not only that KCTD12 plays an important suppressive function and its high level is related with favorable prognosis in gastrointestinal stromal tumors 11 . Abbaszadegan et al showed that KCTD12 can suppress Wnt/Notch signaling, stem cell factors, and chromatin remodelers in esophageal squamous cell carcinoma 12 . Nonetheless, KCTD12 is dramatically upregulated and closely correlated with larger tumor sizes, higher pathologic stages, and poor survival in cervical and lung cancers 13 .…”

Section: Introductionmentioning

confidence: 99%

KCTD12 promotes G1/S transition of breast cancer cell through activating the AKT/FOXO1 signaling

Kuang

Zeng

et al. 2020

Clinical Laboratory Analysis

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Background Sustaining proliferation is the most fundamental step for breast cancer tumor genesis. Accelerated proliferation is usually linked to the uncontrolled cell cycle. However, the internal and external factors linked to the activation of breast cancer cell cycle are still to be investigated. Methods quantitative PCR (qPCR) and Western blotting assay were used to detect the expression of potassium channel tetramerization domain containing 12 (KCTD12) in breast cancer. MTT and colony formation assays were performed to evaluate the effect of KCTD12 on cell proliferation of breast cancer. Anchorage‐independent growth assay was used to examine the in vitro tumorigenesis of breast cancer cells. Flow cytometry assay, qPCR, and Western blotting were used to investigate the detailed mechanisms of KCTD12 on breast cancer progression. Results Herein, the result showed that the level of KCTD12 is significantly decreased in breast cancer tissues and cells, and lower level of KCTD12 predicts poorer survival for patients with breast cancer. Further cell function tests illustrated that downregulation of KCTD12 significantly promotes cell proliferation and in vitro tumor genesis. Besides, molecular biologic experiments showed that downregulation of KCTD12 can enhance the G1/S transition through activating the AKT/FOXO1 signaling. Conclusion Our study inferred that downregulation of KCTD12 can be a novel factor for poor prognosis in breast cancer.

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“…Earlier reports stated that KCTD12 inhibited CRC stemness and metastasis through suppressing the ERK pathway 5 . The latest study found that KCTD12 exerted its inhibitory role in ESCC through inhibiting stem cell factors 7 . However, the CSC hypothesis in melanoma is controversial 12,15 .…”

Section: Discussionmentioning

confidence: 99%

“…Earlier reports declared that KCTD12 promoted the proliferation of human uveal melanoma OCM-1 cells 6 . In addition, it was reported that KCTD12 acted as a tumor suppressor in esophageal squamous cell carcinoma (ESCC) through the WNT/NOTCH pathway and chromatin remodeling 7 . However, the role of KCTD12 in cutaneous melanoma progression has yet to be investigated.…”

Section: Introductionmentioning

confidence: 99%

Downregulation of KCTD12 contributes to melanoma stemness by modulating CD271

Shen

et al. 2019

Cancer Biol Med

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Objective: Cancer metastasis remains the primary cause of cancer-related death worldwide. In a previous study, we found that levels of BTB/POZ domain-containing protein KCTD12 are lower in metastatic melanoma cells than in parental melanoma cells. The purpose of this study was to identify the roles of KCTD12 in cancer metastasis. Methods: The Cancer Genome Atlas (TCGA) datasets were used to evaluate the relationship between KCTD12 and skin cutaneous melanoma (SKCM) prognosis. The effects of endogenous KCTD12 on biological behaviors were examined using the MTT assay. The impacts of KCTD12 on melanoma stemness were explored using spheroid formation assay. KCTD12 knockout A375 cells were generated to confirm the inhibitory effect of KCTD12 on CD271, and a mouse metastatic model was used to determine the impact of KCTD12 on melanoma metastasis in vivo. Results: KCTD12 levels were lower in lung metastatic cells than in paired parental melanoma cells, and low KCTD12 expression indicated a poor prognosis in SKCM. Cancer metastasis-related capacities were higher in lung metastatic cells than in parental melanoma cells. Moreover, KCTD12 knockdown enhanced tumor growth and metastasis both in vitro and in vivo. Mechanistically, the interaction between KCTD12 and CD271 might be responsible for the stemness transformation after KCTD12 knockdown. Conclusions: This study identifies for the first time the role of the interaction between KCTD12 and CD271 in inducing melanoma cell stemness transformation. Moreover, KCTD12 repression enhances melanoma cell growth, adhesion, migration and invasion.

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Contribution of KCTD12 to esophageal squamous cell carcinoma

Cited by 19 publications

References 34 publications

Proteomics Profiling of KAIMRC1 in Comparison to MDA-MB231 and MCF-7

Proteomics Profiling of KAIMRC1 in Comparison to MDA-MB231 and MCF-7

KCTD12 promotes G1/S transition of breast cancer cell through activating the AKT/FOXO1 signaling

Downregulation of KCTD12 contributes to melanoma stemness by modulating CD271

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