Contribution of PBP3 Substitutions and TEM-1, TEM-15, and ROB-1 Beta-Lactamases to Cefotaxime Resistance in <i>Haemophilus influenzae</i> and <i>Haemophilus parainfluenzae</i>

Søndergaard, Annette; Nørskov‐Lauritsen, Niels

doi:10.1089/mdr.2015.0189

Cited by 20 publications

(17 citation statements)

References 23 publications

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“…An Iranian meta-analysis of a collection of 43 articles from different databases showed that the prevalence of H. influenzae that was non-susceptible to cefotaxime was 22.3% [32]. Cefotaxime resistance in H. influenzae is primarily caused by amino acid substitutions N526K, S385T, and L389F and additional substitutions G555E and Y557H in penicillin-binding protein 3 [10,33,34]. Our survey shows that the prevalence of cefotaxime-non-susceptible H. influenzae in Taiwan from 2007 to 2018 (2.7%) is not as high as those in the above-mentioned reports.…”

Section: Discussionmentioning

confidence: 99%

Extensively drug-resistant Haemophilus influenzae – emergence, epidemiology, risk factors, and regimen

Huang

Lai

et al. 2020

BMC Microbiol

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Background: Concern about Haemophilus influenzae infection has been increasing over recent decades. Given the emergence of H. influenzae with severe drug resistance, we assessed the prevalence of as well as risk factors and potential therapies for extensively drug-resistant (XDR) H. influenzae infection in Taiwan. Results: In total, 2091 H. influenzae isolates with disk diffusion-based antibiotic susceptibility testing from 2007 to 2018 were enrolled. H. influenzae strains resistant to ampicillin, chloramphenicol, levofloxacin, and trimethoprimsulfamethoxazole tended to be isolated from patient wards (≧41%), whereas those resistant to amoxicillinclavulanate, cefotaxime, and cefuroxime were more likely to be isolated from intensive care units (approximately 50%). XDR H. influenzae was first identified in 2007, and its incidence did not significantly change thereafter. Overall prevalence of single, multiple, and extensively drug-resistant H. influenzae over 2007-2018 was 21.5% (n = 450), 26.6% (n = 557), and 2.5% (n = 52), respectively. A stepwise logistic regression analysis revealed that blood culture (odds ratio: 4.069, 95% confidence intervals: 1.339-12.365, P = 0.013) was an independent risk factor for XDR H. influenzae infection. No nosocomial transmission of XDR H. influenzae observed. Antibiotic susceptibility testing results demonstrated that cefotaxime was effective against 78.8% (n = 41) of the XDR strains. Conclusions: The presence of XDR H. influenzae strains was identified in Taiwan, and cefotaxime was efficacious against most of these strains.

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Section: Discussionmentioning

confidence: 99%

Extensively drug-resistant Haemophilus influenzae – emergence, epidemiology, risk factors, and regimen

Huang

Lai

et al. 2020

BMC Microbiol

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“…HI strains are highly susceptible to third-generation cephalosporins. Kiedrowska et al assessed antibiotics susceptibility in vitro against 117 Polish HI isolates in 2012 and found that all of the isolates, including β -lactamase-positive HI and BLNAR isolates, were sensitive to ceftriaxone [17]; BLNAR HI with PBP 3 mutations or HI with β -lactamase production may be associated with elevated MIC values of third-generation cephalosporins, but the level did not transgress the resistance breakpoint in most isolates [16–18]. In the present study, the susceptibility rate of HI to cefotaxime/ceftriaxone was as high as 94.1%, which was in accordance with the findings in previous studies [13, 17].…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Resistance Profiles of Haemophilus influenzae Isolates from Children in 2016: A Multicenter Study in China

Wang

Chen

et al. 2019

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Background and Objective. Haemophilus influenzae (HI) is a common cause of community-acquired pneumonia in children. In many countries, HI strains are increasingly resistant to ampicillin and other commonly prescribed antibiotics, posing a challenge for effective clinical treatment. This study was undertaken to determine the antibiotic resistance profiles of HI isolates from Chinese children and to provide guidelines for clinical treatment. Methods. Our Infectious Disease Surveillance of Pediatrics (ISPED) collaboration group includes six children’s hospitals in different regions of China. The same protocols and guidelines were used by all collaborators for the culture and identification of HI. The Kirby–Bauer method was used to test antibiotic susceptibility, and a cefinase disc was used to detect β-lactamase activity. Results. We isolated 2073 HI strains in 2016: 83.9% from the respiratory tract, 11.1% from vaginal secretions, and 0.5% from blood. Patients with respiratory isolates were significantly younger than nonrespiratory patients (P<0.001). Of all 2073 strains, 50.3% were positive for β-lactamase and 58.1% were resistant to ampicillin; 9.3% were β-lactamase-negative and ampicillin-resistant. The resistance rates of the HI isolates to trimethoprim-sulfamethoxazole, azithromycin, cefuroxime, ampicillin-sulbactam, cefotaxime, and meropenem were 71.1%, 32.0%, 31.2%, 17.6%, 5.9%, and 0.2%, respectively. Conclusions. More than half of the HI strains isolated from Chinese children were resistant to ampicillin, primarily due to the production of β-lactamase. Cefotaxime and other third-generation cephalosporins could be the first choice for the treatment of ampicillin-resistant HI infections.

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“…The bla TEM−1 and bla ROB−1 genes were identified by PCR as previously reported, respectively (14). The gene ftsI was sequenced and compared with that of Rd KW20 strain to determined the mutation of PBP3 (15). Isolates with PBP3 mutation patterns were classified to group I, II, III, and III-like as previously reported (9).…”

Section: β-Lactamase and Pbp3 Mutation Detectionmentioning

confidence: 99%

Molecular Epidemiology and Antimicrobial Resistance of Haemophilus influenzae in Adult Patients in Shanghai, China

Xiao

et al. 2020

Front. Public Health

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Background: The serotype and antimicrobial resistance of Haemophilus influenzae in adult patients have changed due to the application of antimicrobials and H. influenzae type b (Hib) vaccine worldwide. However, the epidemiologic characteristics of H. influenzae in Shanghai are still unavailable. Objective: To determine the serotype distribution, antimicrobial resistance and multilocus sequence type (MLST) of H. influenzae in adult patients in Shanghai. Methods: A total of 51 clinical isolates from adult patients were consecutively collected. Serotypes were determined according to specific capsule gene, bexA, amplified by PCR. Antimicrobial susceptibility test was carried out by the broth microdilution method. β-lactamase production was detected by cefinase disk and the ftsI gene were amplified and sequenced to determine the penicillin binding protein 3 (PBP3) mutation. Molecular epidemiology was performed by MLST analyses. Results: All isolates studied were nontypeable H. influenzae (NTHi) and three of them (5.88%) caused invasive infection. The resistant rates of ampicillin and trimethoprim/sulfamethoxazole were both 45.10%. One third of these isolates produced TEM-1 type β-lactamase and 11.76% were β-lactamase negative ampicillin resistant strains (BLNAR). The PBP3 mutation was detected in 74.51% of the isolates, of which 12 belonged to group III. A total of 36 sequence types (STs) were identified among all isolates. Four isolates of ST103 (7.84%) all produced β-lactamase without mutation of PBP3. Conclusion: H. influenzae infections among adults in Shanghai are predominately caused by NTHi with genetic diversity among adult patients. The prevalence of both β-lactamase production and PBP3 mutation may contribute to high ampicillin resistance rate in Shanghai.

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Contribution of PBP3 Substitutions and TEM-1, TEM-15, and ROB-1 Beta-Lactamases to Cefotaxime Resistance in Haemophilus influenzae and Haemophilus parainfluenzae

Abstract: TEM-15 and PBP3 substitutions impose an additive effect on resistance to third-generation cephalosporins in both H. influenzae and H. parainfluenzae. The effect of PBP3 substitutions on beta-lactam resistance in H. parainfluenzae can be addressed by transfer of ftsI genes in vitro.

Cited by 20 publications

References 23 publications

Extensively drug-resistant Haemophilus influenzae – emergence, epidemiology, risk factors, and regimen

Extensively drug-resistant Haemophilus influenzae – emergence, epidemiology, risk factors, and regimen

Antibiotic Resistance Profiles of Haemophilus influenzae Isolates from Children in 2016: A Multicenter Study in China

Molecular Epidemiology and Antimicrobial Resistance of Haemophilus influenzae in Adult Patients in Shanghai, China

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