Contribution of postnatal collagen reorientation to depth-dependent mechanical properties of articular cartilage

Turnhout, Mark C. van; Kranenbarg, S.; Leeuwen, J.L. van

doi:10.1007/s10237-010-0233-7

Cited by 25 publications

(23 citation statements)

References 58 publications

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“…Dedicated finite element models can be an aid for a functional analysis of these effects, e.g. [10,18]. In our previous study [23] we found that differences during development had disappeared in the last sample point (72 weeks).…”

Section: Discussionmentioning

confidence: 98%

“…[10,29,53]. We performed such a functional analysis [18] with our earlier data on collagen orientation remodelling [23]. In that functional analysis [18] we found a marked increase in AC stiffness near the bone, but not at the articular surface.…”

Section: Discussionmentioning

confidence: 99%

“…We performed such a functional analysis [18] with our earlier data on collagen orientation remodelling [23]. In that functional analysis [18] we found a marked increase in AC stiffness near the bone, but not at the articular surface. Thus, the effect of postnatal collagen reorientation is the (further) development of depth-dependent mechanical properties in AC.…”

Section: Discussionmentioning

confidence: 99%

“…The combination of the two data sets provides better tools for functional analysis of the role of the collagen fibre network during development, e.g. by composition based finite element models [10,18,53]. Also, the combination of retardance results and collagen density results (figure 6) enabled us to further illustrate the peculiar nature of the transitional zone in the perinatal animals [20,23].…”

Section: Discussionmentioning

confidence: 99%

“…[3]). Both the predominant orientation in the collagen network and the amount of collagen in the network affect the mechanical behaviour, and thus the functioning, of AC [15-18]. The collagen network remodels between birth and maturity: the adult depth-dependent structure is absent at birth [19-23], collagen type I is replaced by collagen type II [24-26], and collagen densities increase [27-29] with depth-dependent variation [21].…”

Section: Introductionmentioning

confidence: 99%

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Postnatal development of depth-dependent collagen density in ovine articular cartilage

et al. 2010

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BackgroundArticular cartilage (AC) is the layer of tissue that covers the articulating ends of the bones in diarthrodial joints. Adult AC is characterised by a depth-dependent composition and structure of the extracellular matrix that results in depth-dependent mechanical properties, important for the functions of adult AC. Collagen is the most abundant solid component and it affects the mechanical behaviour of AC. The current objective is to quantify the postnatal development of depth-dependent collagen density in sheep (Ovis aries) AC between birth and maturity. We use Fourier transform infra-red micro-spectroscopy to investigate collagen density in 48 sheep divided over ten sample points between birth (stillborn) and maturity (72 weeks). In each animal, we investigate six anatomical sites (caudal, distal and rostral locations at the medial and lateral side of the joint) in the distal metacarpus of a fore leg and a hind leg.ResultsCollagen density increases from birth to maturity up to our last sample point (72 weeks). Collagen density increases at the articular surface from 0.23 g/ml ± 0.06 g/ml (mean ± s.d., n = 48) at 0 weeks to 0.51 g/ml ± 0.10 g/ml (n = 46) at 72 weeks. Maximum collagen density in the deeper cartilage increases from 0.39 g/ml ± 0.08 g/ml (n = 48) at 0 weeks to 0.91 g/ml ± 0.13 g/ml (n = 46) at 72 weeks. Most collagen density profiles at 0 weeks (85%) show a valley, indicating a minimum, in collagen density near the articular surface. At 72 weeks, only 17% of the collagen density profiles show a valley in collagen density near the articular surface. The fraction of profiles with this valley stabilises at 36 weeks.ConclusionsCollagen density in articular cartilage increases in postnatal life with depth-dependent variation, and does not stabilize up to 72 weeks, the last sample point in our study. We find strong evidence for a valley in collagen densities near the articular surface that is present in the youngest animals, but that has disappeared in the oldest animals. We discuss that the retardance valley (as seen with polarised light microscopy) in perinatal animals reflects a decrease in collagen density, as well as a decrease in collagen fibril anisotropy.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Postnatal development of depth-dependent collagen density in ovine articular cartilage

et al. 2010

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Computational Mechanobiology in Cartilage and Bone Tissue Engineering: From Cell Phenotype to Tissue Structure

Nagel

Kelly

2012

Computational Modeling in Tissue Engineering

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Integrating qPLM and biomechanical test data with an anisotropic fiber distribution model and predictions of TGF- $$\upbeta $$ 1 and IGF-1 regulation of articular cartilage fiber modulus

Stender

Raub

Yamauchi

et al. 2012

Biomech Model Mechanobiol

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A continuum mixture model with distinct collagen (COL) and glycosaminoglycan (GAG) elastic constituents was developed for the solid matrix of immature bovine articular cartilage. A continuous COL fiber volume fraction distribution function and a true COL fiber elastic modulus (Ef) were used. Quantitative polarized light microscopy (qPLM) methods were developed to account for the relatively high cell density of immature articular cartilage and used with a novel algorithm that constructs a 3D distribution function from 2D qPLM data. For specimens untreated and cultured in vitro, most model parameters were specified from qPLM analysis and biochemical assay results; consequently, Ef was predicted using an optimization to measured mechanical properties in uniaxial tension and unconfined compression. Analysis of qPLM data revealed a highly anisotropic fiber distribution, with principal fiber orientation parallel to the surface layer. For untreated samples, predicted Ef values were 175 and 422 MPa for superficial (S) and middle (M) zone layers, respectively. TGF-β1 treatment was predicted to increase and decrease Ef values for the S and M layers to 281 and 309 MPa, respectively. IGF-1 treatment was predicted to decrease Ef values for the S and M layers to 22 and 26 MPa, respectively. A novel finding was that distinct native depth-dependent fiber modulus properties were modulated to nearly homogeneous values by TGF-β1 and IGF-1 treatments, with modulated values strongly dependent on treatment.

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Contribution of postnatal collagen reorientation to depth-dependent mechanical properties of articular cartilage

Cited by 25 publications

References 58 publications

Postnatal development of depth-dependent collagen density in ovine articular cartilage

Postnatal development of depth-dependent collagen density in ovine articular cartilage

Computational Mechanobiology in Cartilage and Bone Tissue Engineering: From Cell Phenotype to Tissue Structure

Integrating qPLM and biomechanical test data with an anisotropic fiber distribution model and predictions of TGF- $$\upbeta $$ 1 and IGF-1 regulation of articular cartilage fiber modulus

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