Contribution of protein charge to partitioning in aqueous two-phase systems

Partitioning behavior of silica in the Triton X-100/dextran/water aqueous biphasic system

“…It has been well established that in aqueous biphase systems, coulombic forces can be an important factor in determining the partitioning of biomaterials, especially charged particles [2,37,38]. Walter and Selby [37] found that introducing a positively charged polymer into the PEG/dextran biphase system greatly enhanced the yield of negatively charged dog red blood cells in the phase in which the charged polymer predominated.…”

Aqueous two-phase systems containing urea: Influence of protein structure on protein partitioning

“…These findings will improve the knowledge base for a wide range of emerging fields like refolding in a phase system (Forciniti, 1994;Spears et al, 1995) or the construction of fusion tags to enhance partitioning (Berggren et al, 1999;Malmsten et al, 1998). Further investigations on charge-modified T4-lysozymes (Luther et al, 1994(Luther et al, , 1995Fan et al, 1998) will give us more insight into the so-called "electrostatic potential" of these systems (Grossman et al, 1997(Grossman et al, , 1998Tintinger et al, 1997aTintinger et al, , 1997b. The latter is currently being investigated in collaboration with the research group of Prof. G. Maurer (Univ.…”

“…Properties of T4-lysozyme mutants (Dao Pin et al, 1991;Matthews, 1995 (Cleland et al, 1990(Cleland et al, , 1992Laliberté et al, 1994, Maeda et al, 1995Tams et al, 1996). For the experiments we choose very well-described proteins, the wildtype (WT) and the cysteine free WT*, L99A and V149T mutants of T4-lysozyme (Fan et al, 1998;Matthews et al, 1995). L99A and V149T have an additional mutation in the protein core and are markedly less stable compared to the WT and WT* enzyme against thermal denaturation (Table I).…”

A generalized approach to thermodynamic properties of biomolecules for use in bioseparation process design

“…Factors and mechanisms that cause the distribution of biomolecules over the different phases are poorly understood. The value of the overall distribution coefficient depends on the molecular size [32], charge [33][34][35][36], hydrophobicity [37][38][39], solubility and affinity [40]. However, not all these parameters are equally important, this depends on the chosen system.…”