Contribution of sialic acids to integrin α5β1 functioning in melanoma cells

Kolasińska, Ewa; Janik, Marcelina; Lityń́ska, Anna; Przybyło, Małgorzata

doi:10.1016/j.advms.2019.02.002

Cited by 5 publications

(5 citation statements)

References 56 publications

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“…Immunotherapy development could be based in this approach. Kolasińska et al (2019) also mentioned that sialic acid (SA) monosaccharides are commonly found at the outermost end of the sugar chains of various glycoconjugates. SA external position on cell surface molecules strongly influence cell behavior because SA may interact with other cell surface molecules and ECM proteins.…”

Section: Adhesionmentioning

confidence: 99%

“…The ECM integrins also play an important role in melanoma cell adhesion to fibronectin (FN) as well as melanoma cell migration rate on FN. The main receptor of FN (α5β1) can be modified by the α2-3 and α2-6 linked SA residues and its expression level on melanoma cells was positively associated with the progression of melanoma (Kolasińska et al, 2019). The authors suggest that AFM molecular recognition force spectroscopy could be used to assess the unbinding forces between SA and α2-3 and α2-6 integrins.…”

Section: Adhesionmentioning

confidence: 99%

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Melanoma in the Eyes of Mechanobiology

Brás

Radmacher

Sousa

et al. 2020

Front. Cell Dev. Biol.

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Skin is the largest organ of the human body with several important functions that can be impaired by injury, genetic or chronic diseases. Among all skin diseases, melanoma is one of the most severe, which can lead to death, due to metastization. Mechanotransduction has a crucial role for motility, invasion, adhesion and metastization processes, since it deals with the response of cells to physical forces. Signaling pathways are important to understand how physical cues produced or mediated by the Extracellular Matrix (ECM), affect healthy and tumor cells. During these processes, several molecules in the nucleus and cytoplasm are activated. Melanocytes, keratinocytes, fibroblasts and the ECM, play a crucial role in melanoma formation. This manuscript will address the synergy among melanocytes, keratinocytes, fibroblasts cells and the ECM considering their mechanical contribution and relevance in this disease. Mechanical properties of melanoma cells can also be influenced by pigmentation, which can be associated with changes in stiffness. Mechanical changes can be related with the adhesion, migration, or invasiveness potential of melanoma cells promoting a high metastization capacity of this cancer. Mechanosensing, mechanotransduction, and mechanoresponse will be highlighted with respect to the motility, invasion, adhesion and metastization in melanoma cancer.

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Section: Adhesionmentioning

confidence: 99%

Section: Adhesionmentioning

confidence: 99%

Melanoma in the Eyes of Mechanobiology

Brás

Radmacher

Sousa

et al. 2020

Front. Cell Dev. Biol.

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“…For this dimer, the structure of the integrin alpha linked sialylated glycan has already been identified [68]. α5β1-integrins are also expressed by melanoma cells [69]. In these cells, both integrin subunits are sialylated and influence the binding behavior of the dimer.…”

Section: Integrinsmentioning

confidence: 97%

Insight in Adhesion Protein Sialylation and Microgravity Dependent Cell Adhesion—An Omics Network Approach

Bauer

Gombocz²,

Wehland

et al. 2020

IJMS

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The adhesion behavior of human tissue cells changes in vitro, when gravity forces affecting these cells are modified. To understand the mechanisms underlying these changes, proteins involved in cell-cell or cell-extracellular matrix adhesion, their expression, accumulation, localization, and posttranslational modification (PTM) regarding changes during exposure to microgravity were investigated. As the sialylation of adhesion proteins is influencing cell adhesion on Earth in vitro and in vivo, we analyzed the sialylation of cell adhesion molecules detected by omics studies on cells, which change their adhesion behavior when exposed to microgravity. Using a knowledge graph created from experimental omics data and semantic searches across several reference databases, we studied the sialylation of adhesion proteins glycosylated at their extracellular domains with regards to its sensitivity to microgravity. This way, experimental omics data networked with the current knowledge about the binding of sialic acids to cell adhesion proteins, its regulation, and interactions in between those proteins provided insights into the mechanisms behind our experimental findings, suggesting that balancing the sialylation against the de-sialylation of the terminal ends of the adhesion proteins’ glycans influences their binding activity. This sheds light on the transition from two- to three-dimensional growth observed in microgravity, mirroring cell migration and cancer metastasis in vivo.

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“…Cells of the aortic wall, such as endothelial cells (ECs), vascular smooth muscle cells (VSMCs) and platelets, express cell type-specific integrins under healthy and diseased conditions. The hyperexpression of α5β1 integrin in VSMCs has been shown to promote the metastasis of several types of cancer, such as lung cancer [ 7 , 8 ] and melanoma [ 9 , 10 ]; however, α5β1 integrin also has a tumor-suppressive effect in breast cancer [ 11 – 13 ] and colon cancer cell lines [ 14 , 15 ]. The alterations in integrin α5β1 in patients with AAAD are unknown.…”

Section: Introductionmentioning

confidence: 99%

A decrease in integrin α5β1/FAK is associated with increased apoptosis of aortic smooth muscle cells in acute type a aortic dissection

Xue,

Xing,

Yang

et al. 2024

BMC Cardiovasc Disord

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Background Acute type A aortic dissection (AAAD) is a devastating disease. Human aortic smooth muscle cells (HASMCs) exhibit decreased proliferation and increased apoptosis, and integrin α5β1 and FAK are important proangiogenic factors involved in regulating angiogenesis. The aim of this study was to investigate the role of integrin α5β1 and FAK in patients with AAAD and the potential underlying mechanisms. Methods Aortic tissue samples were obtained from 8 patients with AAAD and 4 organ donors at Zhongshan Hospital of Fudan University. The level of apoptosis in the aortic tissues was assessed by immunohistochemical (IHC) staining and terminal-deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) assays. The expression of integrin α5β1 and FAK was determined. Integrin α5β1 was found to be significantly expressed in HASMCs, and its interaction with FAK was assessed via coimmunoprecipitation (Co-IP) analysis. Proliferation and apoptosis were assessed by Cell Counting Kit-8 (CCK-8) assays and flow cytometry after integrin α5β1 deficiency. Results The levels of integrin α5β1 and FAK were both significantly decreased in patients with AAAD. Downregulating the expression of integrin α5β1-FAK strongly increased apoptosis and decreased proliferation in HASMCs, indicating that integrin α5β1-FAK might play an important role in the development of AAAD. Conclusions Downregulation of integrin α5β1-FAK is associated with increased apoptosis and decreased proliferation in aortic smooth muscle cells and may be a potential therapeutic strategy for AAAD.

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Contribution of sialic acids to integrin α5β1 functioning in melanoma cells

Cited by 5 publications

References 56 publications

Melanoma in the Eyes of Mechanobiology

Melanoma in the Eyes of Mechanobiology

Insight in Adhesion Protein Sialylation and Microgravity Dependent Cell Adhesion—An Omics Network Approach

A decrease in integrin α5β1/FAK is associated with increased apoptosis of aortic smooth muscle cells in acute type a aortic dissection

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