When monolayers of tissue cancer cells of various origins are exposed to real or simulated microgravity, many cells leave the monolayer and assemble to three-dimensional (3D) aggregates (spheroids). In order to define the cellular machinery leading to this change in growth behavior of FTC-133 human thyroid cancer cells and MCF-7 breast cancer cells, we recently performed proteome analyses on these cell lines and determined the proteins’ accumulation in monolayer cells grown under 1g-conditions as well as in the cells of spheroids assembled under simulated microgravity during three and 14 days, respectively. At that time, an influence of the increment or decrement of some of the more than 5000 proteins detected in each cell line was investigated. In this study, we focused on posttranslational modifications (PTMs) of proteins. For this purpose, we selected candidates from the list of the proteins detected in the two preceding proteome analyses, which showed significant accumulation in spheroid cells as compared to 1g monolayer cells. Then we searched for those PTMs of the selected proteins, which according to the literature have already been determined experimentally. Using the Semantic Protocol and RDF Query Language (SPARQL), various databases were examined. Most efficient was the search in the latest version of the dbPTM database. In total, we found 72 different classes of PTMs comprising mainly phosphorylation, glycosylation, ubiquitination and acetylation. Most interestingly, in 35 of the 69 proteins, N6 residues of lysine are modifiable.

Access to consistent, high-quality metadata is critical to finding, understanding, and reusing scientific data. However, while there are many relevant vocabularies for the annotation of a dataset, none sufficiently captures all the necessary metadata. This prevents uniform indexing and querying of dataset repositories. Towards providing a practical guide for producing a high quality description of biomedical datasets, the W3C Semantic Web for Health Care and the Life Sciences Interest Group (HCLSIG) identified Resource Description Framework (RDF) vocabularies that could be used to specify common metadata elements and their value sets. The resulting guideline covers elements of description, identification, attribution, versioning, provenance, and content summarization. This guideline reuses existing vocabularies, and is intended to meet key functional requirements including indexing, discovery, exchange, query, and retrieval of datasets, thereby enabling the publication of FAIR data. The resulting metadata profile is generic and could be used by other domains with an interest in providing machine readable descriptions of versioned datasets.

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Research in natural sciences is mainly done by means of experiments. Some of those experiments such as spaceflight-dependent experiments are extremely laborious, complex and expensive. Hence, they often remain rare events with little chances of statistical tests and possibilities of repetition. In order to make each single event as valuable as possible, a sophisticated comparison of experimental data received with the hundreds of millions of computer-stored documents appears necessary. We used results of an earlier study on proteome analysis of microgravity-exposed human thyroid cancer cells, selected twenty proteins which appeared gravity sensitive and investigated whether their change observed in cells under the loss of gravity could cause health problems in astronauts. Using network analysis via Knowledge Explorer (KE) we searched the literature for diseases related to one or more of the selected proteins. After using Linked Open Data (LOD) and other public resources to establish a comprehensive semantic knowledgebase around functional properties of the selected proteins, the collection's network was used to query a set of databases for the proteins' involvement in biosystems and human diseases. Finally, possible countermeasures could be proposed.