Contribution of spin-trapping EPR techniques for the measurement of NO production in biological systems

Abstract

In this overview of NO spin-trapping methods, we have pointed out their main advantages over frozen solution EPR spectroscopy detection of nitrosylated metalloproteins: improved sensitivity, potential specificity and time-resolution. A few simple ideas concerning EPR methodology have been recalled. Examples of possible artefacts, sometimes unexpected, which can easily be encountered in any analytical methods have been described. New methodological developments have also been mentioned.

Influence of glycemic control on some real-time biomarkers of free radical formation in type 2 diabetic patients: An EPR study

“…32 It is believed that free radicals are directly implicated in the pathogenesis of diabetes and that levels of •NO radicals in type 2 diabetes patients are increased. [33][34][35] A number of studies have demonstrated lower levels of nitrite/nitrate plasma in diabetic patients compared to the healthy controls. 36,37 Diabetic patients often experience elevated glucose levels.…”

Electrochemical Nitric Oxide Sensors for Biological Samples – Principle, Selected Examples and Applications

“…Also, to obtain coherent calibration results, one must work under very strict conditions of time, pH and temperature because even small changes in these parameters can greatly influence the hemoglobin reaction. EPR spectroscopy is also widely used for NO detection through the development of several methods of NO-trapping [17], but its heaviness and poor detection limit (1 mM) make it unsuitable for routine uses. Finally, gas phase chemiluminescence assay [18], based on the chemiluminescent properties of excited NO Ã 2 formed from NO that reacts with ozone, offers real utility and ease for some specific applications, especially for NO exhaled gas.…”

Development of Concept of Organic Paramagnetics in Molecular Biology

“…Дальн ейший прогресс в области использования стабильных парамагнетиков для решения разнообразных биологических задач нашел отражени е в мног очисленных обзорных статьях и монографиях, нап риме р [14][15][16][17][18][19][20][21][22][23][24][25][26][27]. Для динами ческ ой биохимии несомненный и нтерес представляют локальные конформац ионные и зменения белк овых молекул в растворах.…”