Contribution of the different omega-3 fatty acid desaturase genes to the cold response in soybean

Román, Ángela; Andreu, Vanesa; Hernández, M. Luisa; Lagunas, Beatriz; Picorel, Rafael; Martínez-Rivas, José Manuel; Alfonso, Miguel

doi:10.1093/jxb/ers174

Cited by 91 publications

(77 citation statements)

References 40 publications

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“…A well‐characterized phenomenon demonstrates that temperature modulates membrane fluidity, which is the major site of freezing injury (Shewfelt, ; Thomashow, ). It is also known that the role of FADs in cold acclimation in various plant species (Khodakovskaya et al ., ; Román et al ., ; Thomashow, ). In addition, the divergent FAD genes have been associated with synthesis of divergent fatty acid structures that play major role against biotic/abiotic stresses (Cao et al ., ).…”

Section: Discussionmentioning

confidence: 99%

Genome and evolution of the shade‐requiring medicinal herb Panax ginseng

Kim

Jayakodi

Lee

et al. 2018

Plant Biotechnology Journal

153

123

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Summary Panax ginseng C. A. Meyer, reputed as the king of medicinal herbs, has slow growth, long generation time, low seed production and complicated genome structure that hamper its study. Here, we unveil the genomic architecture of tetraploid P. ginseng by de novo genome assembly, representing 2.98 Gbp with 59 352 annotated genes. Resequencing data indicated that diploid Panax species diverged in association with global warming in Southern Asia, and two North American species evolved via two intercontinental migrations. Two whole genome duplications (WGD) occurred in the family Araliaceae (including Panax) after divergence with the Apiaceae, the more recent one contributing to the ability of P. ginseng to overwinter, enabling it to spread broadly through the Northern Hemisphere. Functional and evolutionary analyses suggest that production of pharmacologically important dammarane‐type ginsenosides originated in Panax and are produced largely in shoot tissues and transported to roots; that newly evolved P. ginseng fatty acid desaturases increase freezing tolerance; and that unprecedented retention of chlorophyll a/b binding protein genes enables efficient photosynthesis under low light. A genome‐scale metabolic network provides a holistic view of Panax ginsenoside biosynthesis. This study provides valuable resources for improving medicinal values of ginseng either through genomics‐assisted breeding or metabolic engineering.

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Section: Discussionmentioning

confidence: 99%

Genome and evolution of the shade‐requiring medicinal herb Panax ginseng

Kim

Jayakodi

Lee

et al. 2018

Plant Biotechnology Journal

153

123

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“…Nevertheless, we are still far from understanding how the activity of both plastidial ω-3 desaturases is specifically regulated in response to these stimuli. Most of our conclusions were obtained through the correlation of FAD gene expression levels with changes in the fatty acid content (Gibson et al, 1994;Berberich et al, 1998;Horiguchi et al, 1998;Collados et al, 2006;Martz et al, 2006; 6 Román et al, 2012;Lagunas et al, 2013;Roman et al, 2015). However, our comprehension of desaturase function at the protein level and more concretely, the relative abundance of FAD7 and FAD8 proteins in the different plant tissues and organs, as well as their variation in response to environmental or developmental stimuli is poorly understood.…”

Section: Introductionmentioning

confidence: 91%

Tissue Distribution and Specific Contribution of Arabidopsis FAD7 and FAD8 Plastid Desaturases to the JA- and ABA-Mediated Cold Stress or Defense Responses

Soria-García

Rubio

Lagunas

et al. 2019

Plant and Cell Physiology

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Subject area: (4) proteins, enzymes and metabolism; (2) environmental and stress responses. Abbreviations: ABA, absicic acid; ACP, acyl-carrier protein: BiFC, bimolecular fluorescence complementation; DA. Dienoic fatty acids; DGDG, digalactosyl-diacylglycerol; ER, endoplasmic reticulum; FAE, fatty acid elongase; FAS, fatty acid synthase; GFP, green fluorescent protein; GUS, glucuronidase; JA, jasmonate; LOX, lipooxigenase; MGDG, monogalactosil-diacylglycerol; PG, phosphatidyl-glicerol; RT-qPCR, quantitative real-time PCR; TA, trienoic fatty acid; YFP, yellow fluorescent protein.3To overcome the difficulties to analyze membrane desaturases at the protein level, transgenic Arabidopsis plants expressing the plastidial AtFAD7 and AtFAD8 ω-3 desaturases fused to GFP, under the control of their endogenous promoters, were generated and their tissue relative abundance was studied. Gene expression, GUS promoter activity, immunoblot and confocal microscopy analyses indicated that AtFAD7 is the major ω-3 desaturase in leaves when compared to AtFAD8. This higher abundance of AtFAD7 was consistent with its higher promoter activity and could be related with its specificity for the abundant leaf galactolipids.AtFAD7 was also present in roots but at much lower level than in leaves. Our data suggest that the AtFAD7 protein in roots might participate in the supply of precursors for JA biosynthesis rather than membrane lipid unsaturation. AtFAD7 protein levels increased in response to wounding but not to JA, and decreased upon ABA treatment. Conversely, AtFAD8 protein levels increased upon cold or JA exposure and decreased at high temperatures, but did not respond to ABA or wounding. These results indicated specific and non-redundant roles for the plastidial ω-3 desaturases in defence, temperature stress or phytohormone mediated responses and a tight coordination of their activities between biotic and abiotic stress signalling pathways.Our data suggested that transcriptional regulation was crucial for this coordination. Finally , bimolecular fluorescence complementation analysis showed that both AtFAD7 and AtFAD8 interact with the AtFAD6 ω-6 desaturase in vivo, suggesting that quaternary complexes are involved in trienoic fatty acid production within the plastid membranes.

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“…In plant cells, ALA represents not only a component of storage and membrane lipids, but also is a precursor of the phytohormone jasmonic acid, which is central to the plant response to abiotic and biotic stress (Weber 2002). Several FAD3 and FAD7/8 genes have been identified in plants (Arondel et al 1992;Chi et al 2017;Hernández et al 2016;Radovanovic et al 2014;Román et al 2012;Teixeira et al 2010;Venegas-Calerón et al 2010;Vrinten et al 2005;Xue et al 2018;Yang et al 2012;Yurchenko et al 2014). A number of examples have been presented which confirm that the activity of ω-3 FADs affects the plant stress response: these include the up-regulation by low temperature of a FAD gene in Arabidopsis thaliana plants (Chen et al 2018;Gibson et al 1994) and a demonstration that the over-expression of an ω-3 FAD improves chilling tolerance in tomato (Yu et al 2009).…”

Section: Introductionmentioning

confidence: 99%

The Family of Peanut Fatty Acid Desaturase Genes and a Functional Analysis of Four ω-3 AhFAD3 Members

et al. 2020

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The synthesis of α-linolenic acid (ALA) requires the activity of ω-3 fatty acid desaturases . The quality of peanut oil would be much improved if the content of ALA could be increased. A scan of the peanut genome revealed that it harbored 36 FAD genes, mapping to 16 of the species' 20 chromosomes. A phylogenetic analysis concluded that these genes belonged to six sub-families, namely stearoyl-acyl-acyl carrier protein desaturases (SAD), FAD2, FAD3, FAD4/5, FAD6 and FAD7/8. Of these, FAD3 and FAD7/8 encoded ω-3 FADs, while genes belonging to the other four sub-families encoded ω-6 FADs. Based on RNA-Seq data, each of the 36 FAD genes was shown to be transcribed in non-stressed plants, but there was variation between them with respect to which organs they were transcribed in. Four ω-3 AhFAD3 genes were functionally characterized; when expressed in Arabidopsis thaliana protoplasts, each was localized mainly in the endoplasmic reticulum, while within peanut, the genes were more strongly transcribed in the developing seed than in either the root or the leaf. When constitutively expressed in Arabidopsis thaliana, both the total fatty acid content of the seed and the relative contribution of ALA were increased. The transgenic seedlings also exhibited an improved level of survival when challenged by salinity stress.

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Contribution of the different omega-3 fatty acid desaturase genes to the cold response in soybean

Cited by 91 publications

References 40 publications

Genome and evolution of the shade‐requiring medicinal herb Panax ginseng

Genome and evolution of the shade‐requiring medicinal herb Panax ginseng

Tissue Distribution and Specific Contribution of Arabidopsis FAD7 and FAD8 Plastid Desaturases to the JA- and ABA-Mediated Cold Stress or Defense Responses

The Family of Peanut Fatty Acid Desaturase Genes and a Functional Analysis of Four ω-3 AhFAD3 Members

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