Contributions of ectomycorrhizal fungi in a reclaimed poplar forest (Populus yunnanensis) in an abandoned metal mine tailings pond, southwest China

Xiao, Yinrun; Hu, Na; Wang, Bowen; Zheng, Kuanyu; Zhao, Zhongqiu; Li, Tao

doi:10.1016/j.jhazmat.2023.130962

Cited by 17 publications

(9 citation statements)

References 86 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Notably, it’s suggested that Mortierellaceae contribute to plant growth enhancement ( Escudero-Martinez and Bulgarelli, 2019 ; Ozimek and Hanaka, 2021 ; Li et al, 2023 ) and augment inter-plant competition ( Ozimek and Hanaka, 2021 ; Yang et al, 2022 ), while also playing a crucial role in soil carbon ( Escudero-Martinez and Bulgarelli, 2019 ; Ozimek and Hanaka, 2021 ), nitrogen ( Li et al, 2023 ), and phosphorus cycling ( Sun et al, 2018 ). Importantly, it has been shown that Thelephoraceae , as ectomycorrhizal fungi, can enhance the tolerance, vigor, and biomass of host plants under heavy metal stress, thereby enhancing the adaptability of plants in mining areas ( Yu et al, 2020 ; Yung et al, 2021 ; Xiao Y. et al, 2023 ). The enrichment of these rhizospheric microorganisms might have enhanced the adaptability of A. lavandulaefolia and B. luminifera to the mining area environment, potentially contributing to their dominance in the region.…”

Section: Discussionmentioning

confidence: 99%

Enriched rhizospheric functional microbiome may enhance adaptability of Artemisia lavandulaefolia and Betula luminifera in antimony mining areas

Xing,

Gai,

Xue

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

Dominant native plants are crucial for vegetation reconstruction and ecological restoration of mining areas, though their adaptation mechanisms in stressful environments are unclear. This study focuses on the interactions between dominant indigenous species in antimony (Sb) mining area, Artemisia lavandulaefolia and Betula luminifera, and the microbes in their rhizosphere. The rhizosphere microbial diversity and potential functions of both plants were analyzed through the utilization of 16S, ITS sequencing, and metabarcoding analysis. The results revealed that soil environmental factors, rather than plant species, had a more significant impact on the composition of the rhizosphere microbial community. Soil pH and moisture significantly affected microbial biomarkers and keystone species. Actinobacteria, Proteobacteria and Acidobacteriota, exhibited high resistance to Sb and As, and played a crucial role in the cycling of carbon, nitrogen (N), phosphorus (P), and sulfur (S). The genes participating in N, P, and S cycling exhibited metabolic coupling with those genes associated with Sb and As resistance, which might have enhanced the rhizosphere microbes’ capacity to endure environmental stressors. The enrichment of these rhizosphere functional microbes is the combined result of dispersal limitations and deterministic assembly processes. Notably, the genes related to quorum sensing, the type III secretion system, and chemotaxis systems were significantly enriched in the rhizosphere of plants, especially in B. luminifera, in the mining area. The phylogenetic tree derived from the evolutionary relationships among rhizosphere microbial and chloroplast whole-genome resequencing results, infers both species especially B. luminifera, may have undergone co-evolution with rhizosphere microorganisms in mining areas. These findings offer valuable insights into the dominant native rhizosphere microorganisms that facilitate plant adaptation to environmental stress in mining areas, thereby shedding light on potential strategies for ecological restoration in such environments.

show abstract

Section: Discussionmentioning

confidence: 99%

Enriched rhizospheric functional microbiome may enhance adaptability of Artemisia lavandulaefolia and Betula luminifera in antimony mining areas

Xing,

Gai,

Xue

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…inoculated with the ECM fungus Sphaerosporella brunnea were able to generate significantly higher plant biomass. Root colonization by ectomycorrhizal fungi Bovista limosa PY5 significantly promoted the growth and increased the biomass of Populus yunnanensis trees in a metal mine tailings pond [ 29 ]. The promoting effect of mycorrhizal fungi on plant growth and root development may be related to the increased nutrient absorption and better physiological status of plants [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

“…For example, biotic stress caused by Botrytis cinerea in lettuce induced the activity of CAT, SOD, and glutathione [ 43 ], while Fusarium culmorum in wheat induced the activity of CAT and POD [ 44 ]. Suillus luteus increased the activities of CAT, APX, and SOD in leaves of Quercus acutissima under Cd stress [ 45 ], while Bovistalmosa triggered the antioxidant defense of Populus yunnanensis by enhancing the activities of SOD and CAT [ 29 ]. Consistent with this work, our results also revealed that CG1 and CG2 significantly increased the antioxidant enzyme CAT, POD, and SOD activities in the shoot of Massion’s pine by 60%, 225%, and 9% and in the root by 168%, 200%, and 11% on average, respectively ( Figure 6 ).…”

Section: Discussionmentioning

confidence: 99%

Increased Tolerance of Massion’s pine to Multiple-Toxic-Metal Stress Mediated by Ectomycorrhizal Fungi

Zhang,

Pang

et al. 2023

Plants

View full text Add to dashboard Cite

Pinus massoniana (Massion’s pine), a pioneer tree species, exhibits restoration potential in polluted mining areas. However, the physiological and molecular mechanisms of ectomycorrhizal (ECM) fungi in Massion’s pine adaptability to multiple-toxic-metal stress are still unclear. Hence, Massion’s pine seedlings inoculated with two strains of C. geophilum, which were screened and isolated from a polluted mine area, were cultivated in mine soil for 90 days to investigate the roles of EMF in mediating toxic metal tolerance in host plants. The results showed that compared with the non-inoculation control, C. geophilum (CG1 and CG2) significantly promoted the biomass, root morphology, element absorption, photosynthetic characteristics, antioxidant enzyme activities (CAT, POD, and SOD), and proline content of Massion’s pine seedlings in mine soil. C. geophilum increased the concentrations of Cr, Cd, Pb, and Mn in the roots of Massion’s pine seedlings, with CG1 significantly increasing the concentrations of Pb and Mn by 246% and 162% and CG2 significantly increasing the concentrations of Cr and Pb by 102% and 78%. In contrast, C. geophilum reduced the concentrations of Cr, Cd, Pb, and Mn in the shoots by 14%, 33%, 27%, and 14% on average, respectively. In addition, C. geophilum significantly reduced the transfer factor (TF) of Cr, Cd, Pb, and Mn by 32–58%, 17–26%, 68–75%, and 18–64%, respectively, and the bio-concentration factor (BF) of Cd by 39–71%. Comparative transcriptomic analysis demonstrated that the differently expressed genes (DEGs) were mainly encoding functions involved in “transmembrane transport”, “ion transport”, “oxidation reduction process”, “oxidative phosphorylation”, “carbon metabolism”, “glycolysis/gluconeogenesis”, “photosynthesis”, and “biosynthesis of amino acids.” These results indicate that C. geophilum is able to mitigate toxic metals stress by promoting nutrient uptake, photosynthesis, and plant growth, thereby modulating the antioxidant system to reduce oxidative stress and reducing the transport and enrichment of toxic metals from the root to the shoot of Massion’s pine seedlings.

show abstract

“…Mycorrhizal fungi, an essential symbiont of plant roots, can also enhance the host plants’ ability to tolerate heavy metals. For example, both arbuscular mycorrhizal ( Funneliformis mosseae ) and ectomycorrhizal fungi ( Bovista limosa ) have been shown to significantly improve heavy metal tolerance within Populus yunnanensis [ 20 , 21 ]. Dark septate endophyte (DSE), a prevalent type of fungal colonizer, can also improve the ability of maize plants to tolerate cadmium (Cd).…”

Section: Introductionmentioning

confidence: 99%

The Role of Lignin in the Compartmentalization of Cadmium in Maize Roots Is Enhanced by Mycorrhiza

Lao

Guo

Hao

et al. 2023

JoF

Self Cite

View full text Add to dashboard Cite

In nature, arbuscular mycorrhizal fungi (AMF) play a crucial role in the root systems of plants. They can help enhance the resistance of host plants by improving the compartmentalization of toxic metal contaminants in the cell walls (CWs). However, the functions and responses of various CW subfractions to mycorrhizal colonization under Cd exposure remain unknown. Here we conducted a study to investigate how Cd is stored in the cell walls of maize roots colonized by Funneliformis mosseae. Our findings indicate that inoculating the roots with AMF significantly lowers the amount of Cd in the maize shoots (63.6 ± 6.54 mg kg−1 vs. 45.3 ± 2.19 mg kg−1, p < 0.05) by retaining more Cd in the mycorrhized roots (224.0 ± 17.13 mg kg−1 vs. 289.5 ± 8.75 mg kg−1, p < 0.01). This reduces the adverse effects of excessive Cd on the maize plant. Additional research on the subcellular distribution of Cd showed that AMF colonization significantly improves the compartmentalization of 88.2% of Cd in the cell walls of maize roots, compared to the 80.8% of Cd associated with cell walls in the non-mycorrhizal controls. We observed that the presence of AMF did not increase the amount of Cd in pectin, a primary binding site for cell walls; however, it significantly enhanced the content of lignin and the proportion of Cd in the total root cell walls. This finding is consistent with the increased activity of lignin-related enzymes, such as PAL, 4CL, and laccase, which were also positively impacted by mycorrhizal colonization. Fourier transform infrared spectroscopy (FTIR) results revealed that AMF increased the number and types of functional groups, including −OH/−NH and carboxylate, which chelate Cd in the lignin. Our research shows that AMF can improve the ability of maize plants to tolerate Cd by reducing the amount of Cd transferred from the roots to the shoots. This is achieved by increasing the amount of lignin in the cell walls, which binds with Cd and prevents it from moving through the plant. This is accomplished by activating enzymes related to lignin synthesis and increasing the exposure of Cd-binding functional groups of lignin. However, more direct evidence on the immobilization of Cd in the mycorrhiza-altered cell wall subfractions is needed.

show abstract

Contributions of ectomycorrhizal fungi in a reclaimed poplar forest (Populus yunnanensis) in an abandoned metal mine tailings pond, southwest China

Cited by 17 publications

References 86 publications

Enriched rhizospheric functional microbiome may enhance adaptability of Artemisia lavandulaefolia and Betula luminifera in antimony mining areas

Enriched rhizospheric functional microbiome may enhance adaptability of Artemisia lavandulaefolia and Betula luminifera in antimony mining areas

Increased Tolerance of Massion’s pine to Multiple-Toxic-Metal Stress Mediated by Ectomycorrhizal Fungi

The Role of Lignin in the Compartmentalization of Cadmium in Maize Roots Is Enhanced by Mycorrhiza

Contact Info

Product

Resources

About