Contributions of root cell wall polysaccharides to Cu sequestration in castor (Ricinus communis L.) exposed to different Cu stresses

Ren, Chao; Qi, Yongbo; Huang, Guoyong; Yao, Shiyuan; You, Jinwei; Hu, Hongqing

doi:10.1016/j.jes.2019.08.012

Cited by 32 publications

(17 citation statements)

References 43 publications

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“…Pectin, hemicellulose and cellulose were extracted as described by Yang et al (2011). Galacturonic acid in pectin was determined according to the method of Ren et al (2020). The pectin content was determined using the mhydroxybiphenyl-sulphuric acid colorimetric method; the standard product was galacturonic acid and the absorbance was determined at 520 nm.…”

Section: Cellulose Hemicellulose Pectin and Monosaccharide Content An...mentioning

confidence: 99%

Hydrogen sulphide regulates the growth of tomato root cells by affecting cell wall biosynthesis under CuO NPs stress

Jia

Huang

et al. 2021

Plant Biol J

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Copper oxide nanoparticles (CuO NPs) show strong nano‐toxic effects on organisms. Hydrogen sulphide (H2S) plays a pivotal role in plant response to abiotic stress. In this study, we examine the crucial role of the cell wall as regulated by H2S in response to CuO NPs stress. The digestion method was employed to determine Cu content using atomic absorption spectrometry. The TraKine pro‐tubulin staining kit was used to investigate the microtubule cytoskeleton using confocal laser‐scanning microscopy. Cell wall component analysis utilized the ICS‐3000 HPLC system. Application of H2S reduced growth inhibition caused by CuO NPs. Furthermore, most of the CuO NPs accumulates in roots, indicating a low transfer rate, and H2S significantly decreased CuO NPs content in roots, leaves and stems. Subcellular distribution analysis implied most Cu accumulated in root cell walls, and that H2S reduced the content of Cu in root cell walls. Cortical microtubules in the plasma membrane, guide cell wall biosynthesis. H2S obviously alleviated microtubule cytoskeleton disorders caused by CuO NPs. In addition, the content of cellulose, hemicellulose, pectin and other monosaccharides in root cell walls was reduced by CuO NPs treatment. H2S enhanced the monosaccharide and polysaccharide contents compared with that after CuO NPs treatment. In conclusion, H2S regulates cell wall development in response to CuO NPs stress by stabilizing microtubules. H2S affected Cu distribution and alleviated growth inhibition of tomato seedlings. The research results provide a theoretical basis for further study of nano‐toxicity regulation in plants.

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Section: Cellulose Hemicellulose Pectin and Monosaccharide Content An...mentioning

confidence: 99%

Hydrogen sulphide regulates the growth of tomato root cells by affecting cell wall biosynthesis under CuO NPs stress

Jia

Huang

et al. 2021

Plant Biol J

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“…Metal transporters such as the zinc-regulated transporter AtMTP1 and the yellow-stripe-like (YSL) family transporters are involved in the transport of metal ions out of the cell or into vacuoles (Martins et al 2016). Several researchers have reported compartmentalization of metals in R. communis, mainly in the cell wall of roots and in the leaves (He et al 2020;Ren et al 2020;Ye et al 2018). Recently, He et al (2020) reported that the subcellular distribution of Cd (27.1%-69%) and Zn (39.6%-66.6%) was higher in the cell walls of roots than in the organelles and soluble fraction of R. communis seedlings.…”

Section: Compartmentalizationmentioning

confidence: 99%

“…The plant cell wall is composed of polysaccharides (pectin, hemicelluloses, and cellulose), glycoprotein, and functional groups (-C-N, -COOH, -N-H, -O-H) (Broxterman and Schols 2018; He et al 2020). These components, which have a high affinity for metal ion binding and adsorption, have been observed in R. communis under metal stress (He et al 2020;Ren et al 2020); therefore, the high concentration of metal ions in cell walls may be due to the high capacity of the cell wall components to bind to metal ions under metal stress.…”

Section: Compartmentalizationmentioning

confidence: 99%

“…To combat metal stress, plants possess various defense mechanisms such as antioxidant enzyme production, sequestration or compartmentalization of metals in metabolically inactive parts of the plant, and organic acid exudation that enhances the plant's tolerance against metal stress (Huang et al 2016; Kiran and Prasad 2019a;Ren et al 2020). Several stress-responsive genes have also been identified to aid in stress tolerance.…”

Section: Introductionmentioning

confidence: 99%

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The utilization of Ricinus communis in the phytomanagement of heavy metal contaminated soils

Yeboah

Lü

et al. 2020

Environ. Rev.

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Soil contamination with toxic metals is a major global concern due to their effects on plants and the ecosystem. In contaminated soils, some plant species have the ability to remediate heavy metals. Ricinus communis L., is an industrial crop plant gaining popularity in the remediation of heavy metal-contaminated soils owing to its strong and deep penetrating roots aiding high metal accumulation and large biomass level. R. communis can tolerate high amount of metals by adopting different strategies which include the production of antioxidant enzymes, subcellular localization, and exudation of organic acid. At the molecular level, R. communis can tolerate metal stress by activating stress-responsive genes. Proper selection of metal tolerant R. communis cultivars is effective in the remediation of metal-contaminated soils, owing to their high capacity for metal tolerance. Exogenous application of mineral fertilization and the use of microbes and chelating agents increase metal solubility and availability for plant uptake in soil. Also, good agronomic practices such as co-planting of R. communis with other leguminous crops, enhance R. communis growth and metal tolerance, thereby improving remediation of metal-contaminated soils. This review, therefore, critically discusses the recent approaches in using R. communis to remediate metal-contaminated soils.

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“…The ability of the cell wall to bind Cd depends on functional groups, such as -NH 2 , -COOH, -OH, and -SH present in cell wall polysaccharides [ 26 , 27 ]. Hemicellulose responds fastest to cuprum (Cu) stress, binding more than 80% of the total Cu in the cell wall of Ricinus communis under Cu stress [ 28 ]. The cell wall hemicellulose has been recognized as the major target of aluminum (Al) and Cd accumulation in Arabidopsis [ 29 , 30 ] and Al in Oryza sativa [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

The Role of Hemicellulose in Cadmium Tolerance in Ramie (Boehmeria nivea (L.) Gaud.)

Jie

Tang

et al. 2022

Plants

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Ramie cell walls play an important role in cadmium (Cd) detoxification. However, the Cd binding capacity of the cell wall components and the cell wall compositions among ramie species remains unclear. Therefore, this study compared two ramie populations (‘Dazhuhuangbaima’ (low-Cd-accumulating population) and ‘Zhongzhu 1’ (high-Cd-accumulating population)) with different Cd enrichment characteristics. The two ramie populations were treated with 0, 25, and 75 mg kg−1 Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined. The root length, plant height, biomass, and Cd enrichment in all organs were significantly higher (p ≤ 0.05) in ‘Zhongzhu 1’ than in ‘Dazhuhuangbaima’ under Cd stress. In addition, the subcellular Cd distribution analysis revealed that Cd was mainly found in the cell wall in both ramie populations. Among the cell wall fractions, Cd was mainly bound to the hemicelluloses, with 60.38–73.10% and 50.05–64.45% Cd accumulating in the ‘Zhongzhu 1’ and ‘Dazhuhuangbaima’ cell wall hemicelluloses, respectively. However, the Cd concentration in the ‘Zhongzhu 1’ hemicellulose was significantly higher (p ≤ 0.05) than that in the ‘Dazhuhuangbaima’ hemicellulose. Hemicellulose content analysis further revealed that the hemicellulose concentration increased with the Cd concentration in both populations, but it was significantly higher (p ≤ 0.05) in ‘Zhongzhu 1’ than in ‘Dazhuhuangbaima’ across all Cd treatments. Thus, ramie copes under Cd stress by increasing the hemicellulose content in the cell wall. The findings in this study confirm that hemicellulose is the main enrichment site for Cd in ramie. It also provides a theoretical basis for Cd enrichment breeding in ramie.

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Contributions of root cell wall polysaccharides to Cu sequestration in castor (Ricinus communis L.) exposed to different Cu stresses

Cited by 32 publications

References 43 publications

Hydrogen sulphide regulates the growth of tomato root cells by affecting cell wall biosynthesis under CuO NPs stress

Hydrogen sulphide regulates the growth of tomato root cells by affecting cell wall biosynthesis under CuO NPs stress

The utilization of Ricinus communis in the phytomanagement of heavy metal contaminated soils

The Role of Hemicellulose in Cadmium Tolerance in Ramie (Boehmeria nivea (L.) Gaud.)

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