2018
DOI: 10.3389/fpls.2018.01536
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Contributions of Three Starch Branching Enzyme Isozymes to the Fine Structure of Amylopectin in Rice Endosperm

Abstract: Three starch branching enzyme (BE) isozymes, BEI, BEIIa, and BEIIb, are involved in starch biosynthesis in rice endosperm. Past in vivo and in vitro studies have suggested that each BE isozyme plays a distinct role in forming the fine structure of amylopectin. To elucidate more details of their roles, we prepared DNA constructs in which all the possible combinations of the expressions of these three isozymes were suppressed in developing rice endosperm. Analysis of the chain-length distributions of amylopectin… Show more

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Cited by 46 publications
(38 citation statements)
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“…Generation of non-transgenic rice lines de cient in BEI and BEIIb , and the resulting transgenic lines showed drastic starch phenotypes compared with the WT. However, there are no information about the seed weight or agricultural traits in these transgenic rice lines, except that the materials produced by RNAi technology, which suppressing BEI and BEIIb, signi cantly reduced it seed weight (Sawada et al 2018). Unlike these previous studies, we generated a non-transgenic be1 be2b double mutant rice line for more practical use by crossing single mutant lines that are completely de cient in BEI or BEIIb.…”
Section: Discussionmentioning
confidence: 99%
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“…Generation of non-transgenic rice lines de cient in BEI and BEIIb , and the resulting transgenic lines showed drastic starch phenotypes compared with the WT. However, there are no information about the seed weight or agricultural traits in these transgenic rice lines, except that the materials produced by RNAi technology, which suppressing BEI and BEIIb, signi cantly reduced it seed weight (Sawada et al 2018). Unlike these previous studies, we generated a non-transgenic be1 be2b double mutant rice line for more practical use by crossing single mutant lines that are completely de cient in BEI or BEIIb.…”
Section: Discussionmentioning
confidence: 99%
“…The maize GEMS-0067 lines are thought to be be1 be2b double mutants (Personal communication with Prof. Jane) (Li et al 2008). The expression of BEI and BEIIb has been down-regulated in rice (Zhu et al 2012;Pan et al 2018;Wang et al 2017;Sawada et al 2018), barley (Hordeum vulgare) (Carcio et al 2012), and wheat (Triticum aestivum) (Regina et al 2006). In indica rice, down-regulation of BEI and BEIIb genes resulted in higher apparent contents of amylose (64.8%) and RS (14.6%) than the WT (Zhu et al 2012).…”
Section: Introductionmentioning
confidence: 99%
See 1 more Smart Citation
“…The maize GEMS-0067 lines are thought to be be1 be2b double mutants (Personal communication with Prof. Jane) (Li et al 2008). The expression of BEI and BEIIb has been down-regulated in rice (Zhu et al 2012;Wang et al 2017;Pan et al 2018;Sawada et al 2018), barley (Hordeum vulgare) (Carcio et al 2012), and wheat (Triticum aestivum) (Regina et al 2006). In indica rice, down-regulation of BEI and BEIIb genes resulted in higher apparent contents of amylose (64.8%) and RS (14.6%) than the WT (Zhu et al 2012).…”
Section: Introductionmentioning
confidence: 99%
“…Amylose was preferentially degraded in germinating seeds compared with the long amylopectin chains (Zhu et al 2012;Wang et al 2017;Pan et al 2018). In japonica rice, down-regulation of both BEI and BEIIb genes resulted in much lower seed weight, with fewer intermediate chains , compared with the down-regulation of BEIIb alone (Sawada et al 2018). In transgenic barley, suppression of all three BEs resulted in amylose as the only endosperm starch and a very high RS content of gelatinized starch (65%) (Carcio et al 2012).…”
Section: Introductionmentioning
confidence: 99%